Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/58787 |
Resumo: | Bioengineered soft tissues on any meaningful scale or complexity must incorporate aspects of the functional tissue, namely a vasculature, providing cells oxygen and nutrients critical for their survival. However, the ability of tissue engineering strategies to promote a fast revascularization is critically limited. Particularly in endodontic regenerative therapies, the complicated anatomy of the root canal system, and the narrow apical access limit the supply of new blood vessels and pulp tissue ingrowth. Here we characterize the viscoelastic and microstructural properties of a class of injectable hyaluronic acid (HA) hydrogels formed in situ, reinforced with cellulose nanocrystals (CNCs) and enriched with platelet lysate (PL), and test its ability to promote cells recruitment and proangiogenic activity in vitro. The incorporation of CNCs enhanced the stability of the materials against hydrolytic and enzymatic degradation. Moreover, the release of the chemotactic and pro-angiogenic growth factors (GFs) (PDGF and VEGF) from the PL-laden hydrogels showed an improved sustained profile proportional to the amount of incorporated CNCs. The PL-laden hydrogels exhibited preferential supportive properties of encapsulated human dental pulp cells (hDPCs) in in vitro culture conditions. Finally, PL-laden hydrogels stimulated chemotactic and pro-angiogenic activity by promoting hDPCs recruitment and cell sprouting in hDPCs/human umbilical vein endothelial cell co-cultures in vitro, and in an ex vivo model. These results support the use of the combined system as a scaffold for GFs delivery and cells recruitment, thereby exhibiting great clinical potential in treating injuries in vascularized tissues. |
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Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regenerationDental pulp cellsEndodontic tissue regenerationGrowth factors-controlled releaseInjectable hydrogelMigrationNeo-angiogenesisCiências Médicas::Biotecnologia MédicaEngenharia e Tecnologia::Biotecnologia IndustrialScience & TechnologyBioengineered soft tissues on any meaningful scale or complexity must incorporate aspects of the functional tissue, namely a vasculature, providing cells oxygen and nutrients critical for their survival. However, the ability of tissue engineering strategies to promote a fast revascularization is critically limited. Particularly in endodontic regenerative therapies, the complicated anatomy of the root canal system, and the narrow apical access limit the supply of new blood vessels and pulp tissue ingrowth. Here we characterize the viscoelastic and microstructural properties of a class of injectable hyaluronic acid (HA) hydrogels formed in situ, reinforced with cellulose nanocrystals (CNCs) and enriched with platelet lysate (PL), and test its ability to promote cells recruitment and proangiogenic activity in vitro. The incorporation of CNCs enhanced the stability of the materials against hydrolytic and enzymatic degradation. Moreover, the release of the chemotactic and pro-angiogenic growth factors (GFs) (PDGF and VEGF) from the PL-laden hydrogels showed an improved sustained profile proportional to the amount of incorporated CNCs. The PL-laden hydrogels exhibited preferential supportive properties of encapsulated human dental pulp cells (hDPCs) in in vitro culture conditions. Finally, PL-laden hydrogels stimulated chemotactic and pro-angiogenic activity by promoting hDPCs recruitment and cell sprouting in hDPCs/human umbilical vein endothelial cell co-cultures in vitro, and in an ex vivo model. These results support the use of the combined system as a scaffold for GFs delivery and cells recruitment, thereby exhibiting great clinical potential in treating injuries in vascularized tissues.RECOGNIZE project (UTAP-ICDT/CTM-BIO/0023/2014), project NORTE-01-0145-FEDER-000021. R.M.A. Domingues acknowledges FCT for SFRH/BPD/112459/2015. P.S. Babo acknowledges the project FOOD4CELLS (PTDC/CTM-BIO/4706/2014-POCI-01-0145-FEDER 016716) for his post-doc grantinfo:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoSilva, C. F.Babo, Pedro Miguel SousaGulino, M.Costa, Lígia Francisca RodriguesOliveira, J. M.Silva-Correia, JoanaDomingues, Rui Miguel AndradeReis, R. L.Gomes, Manuela E.2018-092018-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58787engSilva C. F., Babo P. S., Gulino M., Costa L., Oliveira J. M., Silva-Correia J., Domingues R. M. A., Reis R. L., Gomes M. E. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration, Acta Biomaterialia, doi:10.1016/j.actbio.2018.07.035, 20181742-70611878-756810.1016/j.actbio.2018.07.03530031163https://www.sciencedirect.com/science/article/pii/S174270611830429Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:58:51Zoai:repositorium.sdum.uminho.pt:1822/58787Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:37.804427Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration |
title |
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration |
spellingShingle |
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration Silva, C. F. Dental pulp cells Endodontic tissue regeneration Growth factors-controlled release Injectable hydrogel Migration Neo-angiogenesis Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial Science & Technology |
title_short |
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration |
title_full |
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration |
title_fullStr |
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration |
title_full_unstemmed |
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration |
title_sort |
Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration |
author |
Silva, C. F. |
author_facet |
Silva, C. F. Babo, Pedro Miguel Sousa Gulino, M. Costa, Lígia Francisca Rodrigues Oliveira, J. M. Silva-Correia, Joana Domingues, Rui Miguel Andrade Reis, R. L. Gomes, Manuela E. |
author_role |
author |
author2 |
Babo, Pedro Miguel Sousa Gulino, M. Costa, Lígia Francisca Rodrigues Oliveira, J. M. Silva-Correia, Joana Domingues, Rui Miguel Andrade Reis, R. L. Gomes, Manuela E. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, C. F. Babo, Pedro Miguel Sousa Gulino, M. Costa, Lígia Francisca Rodrigues Oliveira, J. M. Silva-Correia, Joana Domingues, Rui Miguel Andrade Reis, R. L. Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Dental pulp cells Endodontic tissue regeneration Growth factors-controlled release Injectable hydrogel Migration Neo-angiogenesis Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial Science & Technology |
topic |
Dental pulp cells Endodontic tissue regeneration Growth factors-controlled release Injectable hydrogel Migration Neo-angiogenesis Ciências Médicas::Biotecnologia Médica Engenharia e Tecnologia::Biotecnologia Industrial Science & Technology |
description |
Bioengineered soft tissues on any meaningful scale or complexity must incorporate aspects of the functional tissue, namely a vasculature, providing cells oxygen and nutrients critical for their survival. However, the ability of tissue engineering strategies to promote a fast revascularization is critically limited. Particularly in endodontic regenerative therapies, the complicated anatomy of the root canal system, and the narrow apical access limit the supply of new blood vessels and pulp tissue ingrowth. Here we characterize the viscoelastic and microstructural properties of a class of injectable hyaluronic acid (HA) hydrogels formed in situ, reinforced with cellulose nanocrystals (CNCs) and enriched with platelet lysate (PL), and test its ability to promote cells recruitment and proangiogenic activity in vitro. The incorporation of CNCs enhanced the stability of the materials against hydrolytic and enzymatic degradation. Moreover, the release of the chemotactic and pro-angiogenic growth factors (GFs) (PDGF and VEGF) from the PL-laden hydrogels showed an improved sustained profile proportional to the amount of incorporated CNCs. The PL-laden hydrogels exhibited preferential supportive properties of encapsulated human dental pulp cells (hDPCs) in in vitro culture conditions. Finally, PL-laden hydrogels stimulated chemotactic and pro-angiogenic activity by promoting hDPCs recruitment and cell sprouting in hDPCs/human umbilical vein endothelial cell co-cultures in vitro, and in an ex vivo model. These results support the use of the combined system as a scaffold for GFs delivery and cells recruitment, thereby exhibiting great clinical potential in treating injuries in vascularized tissues. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09 2018-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/58787 |
url |
http://hdl.handle.net/1822/58787 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva C. F., Babo P. S., Gulino M., Costa L., Oliveira J. M., Silva-Correia J., Domingues R. M. A., Reis R. L., Gomes M. E. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration, Acta Biomaterialia, doi:10.1016/j.actbio.2018.07.035, 2018 1742-7061 1878-7568 10.1016/j.actbio.2018.07.035 30031163 https://www.sciencedirect.com/science/article/pii/S174270611830429X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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