Gingipains as a virulence factor in the oral cavity

Detalhes bibliográficos
Autor(a) principal: Lopes, Pedro Campos
Data de Publicação: 2012
Outros Autores: Barros, Marlene, Correia, Maria José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/35508
Resumo: Aim: The objective of this study is to demonstrate the molecular action of Porphyromonas gingivalis cysteine proteases such as gingipains (R1, R2 and K) upon human molecules. Materials and methods: Using the information on protein structure and function available in international databases (UniProtKB and Merops Database), the molecular interactions already described between gingipains and host molecules were clarified. Results: Possible cleavage sites were identified in host-produced elastase inhibitors and in pro-Matrix MetalloProteinase (MMP)1. Analysis of the results leads to the suggestion that the elastase inhibitor alpha1-antitrypsin is also degraded by interpain A, a cystein protease of Prevotella intermedia sharing a high homology with the PrtT and periodontain of P. gingivalis. Conclusion: The information obtained suggests a synergistic molecular mechanism by which cysteine proteases of different bacteria can be responsible for the clinical manifestations of periodontal disease, and illustrates the use of bioinformatics to establish and predict molecular mechanisms.
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spelling Gingipains as a virulence factor in the oral cavityGingipains como fatores de virulência na cavidade oralBioinformaticsElastasePeriodontitisPorphyromonas gingivalisProteasesPeriodontiteBioinformáticaElastaseAim: The objective of this study is to demonstrate the molecular action of Porphyromonas gingivalis cysteine proteases such as gingipains (R1, R2 and K) upon human molecules. Materials and methods: Using the information on protein structure and function available in international databases (UniProtKB and Merops Database), the molecular interactions already described between gingipains and host molecules were clarified. Results: Possible cleavage sites were identified in host-produced elastase inhibitors and in pro-Matrix MetalloProteinase (MMP)1. Analysis of the results leads to the suggestion that the elastase inhibitor alpha1-antitrypsin is also degraded by interpain A, a cystein protease of Prevotella intermedia sharing a high homology with the PrtT and periodontain of P. gingivalis. Conclusion: The information obtained suggests a synergistic molecular mechanism by which cysteine proteases of different bacteria can be responsible for the clinical manifestations of periodontal disease, and illustrates the use of bioinformatics to establish and predict molecular mechanisms.Objetivo: O objetivo deste trabalho é verificar o detalhe molecular da ação de proteases de cisteína de Porphyromonas gingivalis como as gingipains (R1, R2 e K) em moléculas do hospedeiro. Material e métodos: Utilizando a informação disponível sobre estrutura e função de proteínas nas bases de dados internacionais (UniProtKB e Merops Database) as interações já descritas entre gingipains e moléculas do hospedeiro são clarificadas. Resultados: São identificados possíveis locais de corte das gingipains em inibidores naturais das elastases e a identificação molecular de um local de corte na pro MetaloProteinase da Matriz (MMP) 1. A análise dos resultados sugere que a interpain A, uma protease de cisteína de Prevotella intermedia com elevada homologia estrutural com as proteases de cisteína PrtT e periodontain de P. gingivalis, também degrada o inibidor de elastases, alfa1antitripsina. Conclusão Com a integração da informação obtida, sugere-se um possível mecanismo molecular da sinergia criada entre proteinases de cisteína no sentido de promover a doença periodontal. Este estudo ilustra a forma como as ferramentas bioinformáticas podem ser úteis no esclarecimento dos mecanismos moleculares e na previsão de resultados experimentais, melhorando o desenho de estudos laboratoriais.Veritati - Repositório Institucional da Universidade Católica PortuguesaLopes, Pedro CamposBarros, MarleneCorreia, Maria José2021-10-12T13:50:55Z2012-102012-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/35508eng1646-289010.1016/j.rpemd.2012.07.00284870239265info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:41:02Zoai:repositorio.ucp.pt:10400.14/35508Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:28:50.416010Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gingipains as a virulence factor in the oral cavity
Gingipains como fatores de virulência na cavidade oral
title Gingipains as a virulence factor in the oral cavity
spellingShingle Gingipains as a virulence factor in the oral cavity
Lopes, Pedro Campos
Bioinformatics
Elastase
Periodontitis
Porphyromonas gingivalis
Proteases
Periodontite
Bioinformática
Elastase
title_short Gingipains as a virulence factor in the oral cavity
title_full Gingipains as a virulence factor in the oral cavity
title_fullStr Gingipains as a virulence factor in the oral cavity
title_full_unstemmed Gingipains as a virulence factor in the oral cavity
title_sort Gingipains as a virulence factor in the oral cavity
author Lopes, Pedro Campos
author_facet Lopes, Pedro Campos
Barros, Marlene
Correia, Maria José
author_role author
author2 Barros, Marlene
Correia, Maria José
author2_role author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Lopes, Pedro Campos
Barros, Marlene
Correia, Maria José
dc.subject.por.fl_str_mv Bioinformatics
Elastase
Periodontitis
Porphyromonas gingivalis
Proteases
Periodontite
Bioinformática
Elastase
topic Bioinformatics
Elastase
Periodontitis
Porphyromonas gingivalis
Proteases
Periodontite
Bioinformática
Elastase
description Aim: The objective of this study is to demonstrate the molecular action of Porphyromonas gingivalis cysteine proteases such as gingipains (R1, R2 and K) upon human molecules. Materials and methods: Using the information on protein structure and function available in international databases (UniProtKB and Merops Database), the molecular interactions already described between gingipains and host molecules were clarified. Results: Possible cleavage sites were identified in host-produced elastase inhibitors and in pro-Matrix MetalloProteinase (MMP)1. Analysis of the results leads to the suggestion that the elastase inhibitor alpha1-antitrypsin is also degraded by interpain A, a cystein protease of Prevotella intermedia sharing a high homology with the PrtT and periodontain of P. gingivalis. Conclusion: The information obtained suggests a synergistic molecular mechanism by which cysteine proteases of different bacteria can be responsible for the clinical manifestations of periodontal disease, and illustrates the use of bioinformatics to establish and predict molecular mechanisms.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
2012-10-01T00:00:00Z
2021-10-12T13:50:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/35508
url http://hdl.handle.net/10400.14/35508
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1646-2890
10.1016/j.rpemd.2012.07.002
84870239265
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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