Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.25758/set.2051 |
Resumo: | Introduction – Fungal spores are one of the major biological components of the air but are still poorly studied and their influence on respiratory allergic disease is not recognized. In this work, we intend to determine the importance of the atmospheric spores of Alternaria and Cladosporium in respiratory allergic disease in Beja. Methods – The atmospheric levels of Alternaria and Cladosporium spores in Beja were analyzed. The methodology used and recommended by the Portuguese Aerobiology Network was used: a Hirst volumetric collector, a Burkard seven-day Volumetric Spore-trape®; the spores were collected on a reel containing a Melinex tape impregnated with silicone and stained with a glycerogelatin solution with basic fuchsin. For the reading of the samples was used the method of reading the four longitudinal lines to the optical microscope with a magnification of 400x. The results obtained were converted and expressed in the number of spores per cubic meter of air. Allergic patients with fungal allergens with positive mx1 allergen sensitization were selected and a molecular diagnosis of allergen sensitization to Alt a1 was performed via the Phadia 250® device by the fluoroenzyme immunoassay method. Results – During the study period, 320,862 Cladosporium spores and 20,741 spores of the Alternaria type were recorded. Molecular diagnostic tests have confirmed that 81% of patients sensitized to fungi are allergic to Alternaria. Conclusion – The atmospheric levels of Alternaria are high and cause allergic sensitization in patients in the Beja region. The Alt a1 allergen allows the identification of most fungal allergic patients in this region. |
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Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic diseaseNíveis atmosféricos de esporos de Alternaria e Cladosporium na cidade de Beja e seu envolvimento na doença alérgica respiratóriaEsporos fúngicosAlternariaCladosporiumAlt a1Alergia respiratóriaFungal sporesAlternariaCladosporiumAlt a1Respiratory allergic diseaseIntroduction – Fungal spores are one of the major biological components of the air but are still poorly studied and their influence on respiratory allergic disease is not recognized. In this work, we intend to determine the importance of the atmospheric spores of Alternaria and Cladosporium in respiratory allergic disease in Beja. Methods – The atmospheric levels of Alternaria and Cladosporium spores in Beja were analyzed. The methodology used and recommended by the Portuguese Aerobiology Network was used: a Hirst volumetric collector, a Burkard seven-day Volumetric Spore-trape®; the spores were collected on a reel containing a Melinex tape impregnated with silicone and stained with a glycerogelatin solution with basic fuchsin. For the reading of the samples was used the method of reading the four longitudinal lines to the optical microscope with a magnification of 400x. The results obtained were converted and expressed in the number of spores per cubic meter of air. Allergic patients with fungal allergens with positive mx1 allergen sensitization were selected and a molecular diagnosis of allergen sensitization to Alt a1 was performed via the Phadia 250® device by the fluoroenzyme immunoassay method. Results – During the study period, 320,862 Cladosporium spores and 20,741 spores of the Alternaria type were recorded. Molecular diagnostic tests have confirmed that 81% of patients sensitized to fungi are allergic to Alternaria. Conclusion – The atmospheric levels of Alternaria are high and cause allergic sensitization in patients in the Beja region. The Alt a1 allergen allows the identification of most fungal allergic patients in this region.Introdução – Os esporos fúngicos são uma das maiores componentes biológicas atmosféricas; no entanto, são ainda pouco estudados e não lhes é reconhecida a influência devida na doença alérgica respiratória. Neste trabalho pretende-se determinar a importância dos níveis de esporos atmosféricos de Alternaria e Cladosporium na doença alérgica respiratória na cidade de Beja. Método – Analisaram-se os níveis atmosféricos de esporos de Alternaria e Cladosporium na cidade de Beja. A metodologia utilizada é recomendada pela Rede Portuguesa de Aerobiologia: utilizou-se um coletor volumétrico do tipo Hirst, um Burkard seven-day Volumetric Spore-trape®; os esporos foram coletados numa bobine que continha uma fita de Melinex impregnada com silicone e corados com solução de glicerogelatina com fucsina básica. Para a leitura das amostras utilizou-se o método de leitura das quatro linhas longitudinais ao microscópio ótico com uma ampliação de 400x. Os resultados obtidos foram convertidos e expressos em número de esporos por metro cúbico de ar. Selecionaram-se doentes alérgicos a fungos com provas de sensibilização positivas para o alergénio mx1 e realizou-se uma análise de diagnóstico molecular de sensibilização ao alergénio Alt a1 através do equipamento Phadia 250® pelo método de imunoensaio fluoroenzimático. Resultados – No período deste estudo registaram-se 320.862 esporos do tipo Cladosporium e 20.741 esporos do tipo Alternaria. Os testes de diagnóstico molecular confirmaram que 81% dos doentes sensibilizados a fungos são alérgicos a Alternaria. Conclusão – Os níveis atmosféricos de Alternaria são elevados e provocam sensibilização alérgica nos doentes da região de Beja. O alergénio Alt a1 permite identificar a maioria dos doentes alérgicos a fungos nesta região.Escola Superior de Tecnologia da Saúde de Lisboa (Instituto Politécnico de Lisboa)2022-08-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.25758/set.2051oai:journals.ipl.pt:article/564Saúde e Tecnologia; No. 18 (2017): Novembro 2017; 17-22Saúde & Tecnologia; N.º 18 (2017): Novembro 2017; 17-221646-9704reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPporhttps://journals.ipl.pt/stecnologia/article/view/564https://doi.org/10.25758/set.2051https://journals.ipl.pt/stecnologia/article/view/564/491Direitos de Autor (c) 2022 Saúde & Tecnologiainfo:eu-repo/semantics/openAccessAlmeida, ElsaCaeiro, ElsaTodo-Bom, AnaGazarini, LuizDuarte, Ana2022-12-20T10:58:53Zoai:journals.ipl.pt:article/564Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:21:23.130801Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease Níveis atmosféricos de esporos de Alternaria e Cladosporium na cidade de Beja e seu envolvimento na doença alérgica respiratória |
title |
Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease |
spellingShingle |
Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease Almeida, Elsa Esporos fúngicos Alternaria Cladosporium Alt a1 Alergia respiratória Fungal spores Alternaria Cladosporium Alt a1 Respiratory allergic disease |
title_short |
Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease |
title_full |
Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease |
title_fullStr |
Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease |
title_full_unstemmed |
Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease |
title_sort |
Atmospheric levels of Alternaria and Cladosporium spores in the city of Beja and their involvement in respiratory allergic disease |
author |
Almeida, Elsa |
author_facet |
Almeida, Elsa Caeiro, Elsa Todo-Bom, Ana Gazarini, Luiz Duarte, Ana |
author_role |
author |
author2 |
Caeiro, Elsa Todo-Bom, Ana Gazarini, Luiz Duarte, Ana |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Almeida, Elsa Caeiro, Elsa Todo-Bom, Ana Gazarini, Luiz Duarte, Ana |
dc.subject.por.fl_str_mv |
Esporos fúngicos Alternaria Cladosporium Alt a1 Alergia respiratória Fungal spores Alternaria Cladosporium Alt a1 Respiratory allergic disease |
topic |
Esporos fúngicos Alternaria Cladosporium Alt a1 Alergia respiratória Fungal spores Alternaria Cladosporium Alt a1 Respiratory allergic disease |
description |
Introduction – Fungal spores are one of the major biological components of the air but are still poorly studied and their influence on respiratory allergic disease is not recognized. In this work, we intend to determine the importance of the atmospheric spores of Alternaria and Cladosporium in respiratory allergic disease in Beja. Methods – The atmospheric levels of Alternaria and Cladosporium spores in Beja were analyzed. The methodology used and recommended by the Portuguese Aerobiology Network was used: a Hirst volumetric collector, a Burkard seven-day Volumetric Spore-trape®; the spores were collected on a reel containing a Melinex tape impregnated with silicone and stained with a glycerogelatin solution with basic fuchsin. For the reading of the samples was used the method of reading the four longitudinal lines to the optical microscope with a magnification of 400x. The results obtained were converted and expressed in the number of spores per cubic meter of air. Allergic patients with fungal allergens with positive mx1 allergen sensitization were selected and a molecular diagnosis of allergen sensitization to Alt a1 was performed via the Phadia 250® device by the fluoroenzyme immunoassay method. Results – During the study period, 320,862 Cladosporium spores and 20,741 spores of the Alternaria type were recorded. Molecular diagnostic tests have confirmed that 81% of patients sensitized to fungi are allergic to Alternaria. Conclusion – The atmospheric levels of Alternaria are high and cause allergic sensitization in patients in the Beja region. The Alt a1 allergen allows the identification of most fungal allergic patients in this region. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.25758/set.2051 oai:journals.ipl.pt:article/564 |
url |
https://doi.org/10.25758/set.2051 |
identifier_str_mv |
oai:journals.ipl.pt:article/564 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://journals.ipl.pt/stecnologia/article/view/564 https://doi.org/10.25758/set.2051 https://journals.ipl.pt/stecnologia/article/view/564/491 |
dc.rights.driver.fl_str_mv |
Direitos de Autor (c) 2022 Saúde & Tecnologia info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos de Autor (c) 2022 Saúde & Tecnologia |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola Superior de Tecnologia da Saúde de Lisboa (Instituto Politécnico de Lisboa) |
publisher.none.fl_str_mv |
Escola Superior de Tecnologia da Saúde de Lisboa (Instituto Politécnico de Lisboa) |
dc.source.none.fl_str_mv |
Saúde e Tecnologia; No. 18 (2017): Novembro 2017; 17-22 Saúde & Tecnologia; N.º 18 (2017): Novembro 2017; 17-22 1646-9704 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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