Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/7831 |
Resumo: | Aspergillus section Fumigati is reported in up to 99% of aspergillosis cases in penguins. So far, no data regarding molecular epidemiology and azole resistance are available for A. fumigatus isolates collected from Magellanic penguins. The aim of this work was to perform molecular identification of Aspergillus section Fumigati at species level, to genotype those isolates using microsatellite markers, to evaluate the in vitro susceptibility patterns of A. fumigatus sensu stricto, and to characterize the cyp51A gene in clinical A. fumigatus strains isolated from Magellanic penguins with proven aspergillosis. All 34 isolates included in the study were identified as A. fumigatus sensu stricto. Analyzing the genetic diversity of the isolates of A. fumigatus sensu stricto, we identified two possible outbreaks in the rehabilitation center and we also observed the maintenance of clonal strains through the years. One A. fumigatus sensu stricto isolate was resistant to posaconazole, but the mutations found in the cyp51A gene of this isolate have not been described as conferring phenotypic resistance, suggesting that other mechanisms of resistance could be involved in the resistance of this isolate. With this study, we were able to understand the molecular diversity of Aspergillus fumigatus isolates collected from Magellanic penguins, to characterize them and to associate them with the described global population of Aspergillus fumigatus. |
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Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolatesMolecular IdentificationPenguinsMechanisms of ResistanceAspergillus ResistanceMicrosatellite MarkersGenomic DiversityGenotypingInfecções Sistémicas e ZoonosesAspergillus section Fumigati is reported in up to 99% of aspergillosis cases in penguins. So far, no data regarding molecular epidemiology and azole resistance are available for A. fumigatus isolates collected from Magellanic penguins. The aim of this work was to perform molecular identification of Aspergillus section Fumigati at species level, to genotype those isolates using microsatellite markers, to evaluate the in vitro susceptibility patterns of A. fumigatus sensu stricto, and to characterize the cyp51A gene in clinical A. fumigatus strains isolated from Magellanic penguins with proven aspergillosis. All 34 isolates included in the study were identified as A. fumigatus sensu stricto. Analyzing the genetic diversity of the isolates of A. fumigatus sensu stricto, we identified two possible outbreaks in the rehabilitation center and we also observed the maintenance of clonal strains through the years. One A. fumigatus sensu stricto isolate was resistant to posaconazole, but the mutations found in the cyp51A gene of this isolate have not been described as conferring phenotypic resistance, suggesting that other mechanisms of resistance could be involved in the resistance of this isolate. With this study, we were able to understand the molecular diversity of Aspergillus fumigatus isolates collected from Magellanic penguins, to characterize them and to associate them with the described global population of Aspergillus fumigatus.Plain Language Summary: A. fumigatus sensu stricto is of great importance in penguins’ aspergillosis. We could identify two outbreaks in the rehabilitation center and the maintenance of clonal strains through the years. Regarding antifungal prophylaxis, it may proceed, but preferably with surveillance for azole resistance.This study was supported by the International Cooperation Program financed by the Brazilian Federal Agency for Support and Evaluation of Graduate Education within the Ministry of Education of Brazil (CAPES), under the PDSE Program - Finance Code 001.Oxford University Press/ International Society for Human and Animal MycologyRepositório Científico do Instituto Nacional de SaúdeMelo, Aryse MartinsPoester, Vanice RodriguesCanabarro, Paula LimaSampaio, Daniel AtaídeStevens, David AVeríssimo, CristinaSabino, RaquelXavier, Melissa Orzechowski2021-12-23T16:15:13Z2021-11-032021-11-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7831engMed Mycol. 2021 Nov 3;59(11):1076-1084. doi: 10.1093/mmy/myab0401369-378610.1093/mmy/myab040info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:17Zoai:repositorio.insa.pt:10400.18/7831Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:33.740486Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates |
title |
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates |
spellingShingle |
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates Melo, Aryse Martins Molecular Identification Penguins Mechanisms of Resistance Aspergillus Resistance Microsatellite Markers Genomic Diversity Genotyping Infecções Sistémicas e Zoonoses |
title_short |
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates |
title_full |
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates |
title_fullStr |
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates |
title_full_unstemmed |
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates |
title_sort |
Molecular epidemiology of aspergillosis in Magellanic penguins and susceptibility patterns of clinical isolates |
author |
Melo, Aryse Martins |
author_facet |
Melo, Aryse Martins Poester, Vanice Rodrigues Canabarro, Paula Lima Sampaio, Daniel Ataíde Stevens, David A Veríssimo, Cristina Sabino, Raquel Xavier, Melissa Orzechowski |
author_role |
author |
author2 |
Poester, Vanice Rodrigues Canabarro, Paula Lima Sampaio, Daniel Ataíde Stevens, David A Veríssimo, Cristina Sabino, Raquel Xavier, Melissa Orzechowski |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Melo, Aryse Martins Poester, Vanice Rodrigues Canabarro, Paula Lima Sampaio, Daniel Ataíde Stevens, David A Veríssimo, Cristina Sabino, Raquel Xavier, Melissa Orzechowski |
dc.subject.por.fl_str_mv |
Molecular Identification Penguins Mechanisms of Resistance Aspergillus Resistance Microsatellite Markers Genomic Diversity Genotyping Infecções Sistémicas e Zoonoses |
topic |
Molecular Identification Penguins Mechanisms of Resistance Aspergillus Resistance Microsatellite Markers Genomic Diversity Genotyping Infecções Sistémicas e Zoonoses |
description |
Aspergillus section Fumigati is reported in up to 99% of aspergillosis cases in penguins. So far, no data regarding molecular epidemiology and azole resistance are available for A. fumigatus isolates collected from Magellanic penguins. The aim of this work was to perform molecular identification of Aspergillus section Fumigati at species level, to genotype those isolates using microsatellite markers, to evaluate the in vitro susceptibility patterns of A. fumigatus sensu stricto, and to characterize the cyp51A gene in clinical A. fumigatus strains isolated from Magellanic penguins with proven aspergillosis. All 34 isolates included in the study were identified as A. fumigatus sensu stricto. Analyzing the genetic diversity of the isolates of A. fumigatus sensu stricto, we identified two possible outbreaks in the rehabilitation center and we also observed the maintenance of clonal strains through the years. One A. fumigatus sensu stricto isolate was resistant to posaconazole, but the mutations found in the cyp51A gene of this isolate have not been described as conferring phenotypic resistance, suggesting that other mechanisms of resistance could be involved in the resistance of this isolate. With this study, we were able to understand the molecular diversity of Aspergillus fumigatus isolates collected from Magellanic penguins, to characterize them and to associate them with the described global population of Aspergillus fumigatus. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-23T16:15:13Z 2021-11-03 2021-11-03T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/7831 |
url |
http://hdl.handle.net/10400.18/7831 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Med Mycol. 2021 Nov 3;59(11):1076-1084. doi: 10.1093/mmy/myab040 1369-3786 10.1093/mmy/myab040 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press/ International Society for Human and Animal Mycology |
publisher.none.fl_str_mv |
Oxford University Press/ International Society for Human and Animal Mycology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132170766254080 |