Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing

Detalhes bibliográficos
Autor(a) principal: Moura, Maria Eduarda Nogueira
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/30856
Resumo: This internship allowed integration in the ValorMar project, aimed at improving aquaculture industries while also providing health benefits to humans. Aquaculture generates great amounts of waste and by-products, which constitutes an economic, health and environmental problem. These by-products are known to possess nutritional and bioactive potential, worthy of exploration for the manufacture of novel functional foods, that can exert specific beneficial effects on human health and wellbeing. Therefore, the goal of the present work was to explore the bioactive potential of the Mediterranean mussel (Mytilus galloprovincialis), a common European aquaculture shellfish product, that generates high amounts of waste, through enzymatic hydrolysis, to extract potentially bioactive compounds that can later be applied in the creation of new functional foods. Mussel meat homogenates were subjected to hydrolysis with Alcalase® and Protamex®, at pH 7.5, temperature of 60 °C, with different enzyme concentrations (0, 1, 2 and 3%), substrate amounts (25 and 50 g) and duration (1 and 2h). Resultant hydrolysates were further processed through lyophilization or Amicon® ultrafiltration. Pre-thermal-treatment, hydrolysis and hydrolysates processing conditions were evaluated to understand which would lead to the production of bioactive peptides. Hydrolysates were tested for their antioxidant ability, through 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging method, antimicrobial activity, by disk and well diffusion techniques, and for antihypertensive ability by their capacity in inhibiting angiotensin-converting enzyme (iACE). Produced hydrolysates revealed that multiple enzyme hydrolysis produces higher bioactive potential. DPPH scavenging ability increased with substrate and revealed lower activity with the >3 kDa fraction, unlike iACE potential, which increased with the ultrafiltered fraction. In general, boiled mussel hydrolysates showed high antihypertensive potential. However, no antimicrobial effect was observed from samples obtained in the tested conditions. Further studies should be done, widening hydrolysis and purification conditions, testing for different bioactive potential, to gather more knowledge on this species potential as a source of bioactive compounds. Further assays are required to assess hydrolysates stability in food matrices and during digestion, and their effect on target sites.
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spelling Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processingAlcalaseAntihypertensive activityAntimicrobial activityAntioxidant activityBioactive peptidesEnzymatic hydrolysisMytilus galloprovincialisProtamexThis internship allowed integration in the ValorMar project, aimed at improving aquaculture industries while also providing health benefits to humans. Aquaculture generates great amounts of waste and by-products, which constitutes an economic, health and environmental problem. These by-products are known to possess nutritional and bioactive potential, worthy of exploration for the manufacture of novel functional foods, that can exert specific beneficial effects on human health and wellbeing. Therefore, the goal of the present work was to explore the bioactive potential of the Mediterranean mussel (Mytilus galloprovincialis), a common European aquaculture shellfish product, that generates high amounts of waste, through enzymatic hydrolysis, to extract potentially bioactive compounds that can later be applied in the creation of new functional foods. Mussel meat homogenates were subjected to hydrolysis with Alcalase® and Protamex®, at pH 7.5, temperature of 60 °C, with different enzyme concentrations (0, 1, 2 and 3%), substrate amounts (25 and 50 g) and duration (1 and 2h). Resultant hydrolysates were further processed through lyophilization or Amicon® ultrafiltration. Pre-thermal-treatment, hydrolysis and hydrolysates processing conditions were evaluated to understand which would lead to the production of bioactive peptides. Hydrolysates were tested for their antioxidant ability, through 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging method, antimicrobial activity, by disk and well diffusion techniques, and for antihypertensive ability by their capacity in inhibiting angiotensin-converting enzyme (iACE). Produced hydrolysates revealed that multiple enzyme hydrolysis produces higher bioactive potential. DPPH scavenging ability increased with substrate and revealed lower activity with the >3 kDa fraction, unlike iACE potential, which increased with the ultrafiltered fraction. In general, boiled mussel hydrolysates showed high antihypertensive potential. However, no antimicrobial effect was observed from samples obtained in the tested conditions. Further studies should be done, widening hydrolysis and purification conditions, testing for different bioactive potential, to gather more knowledge on this species potential as a source of bioactive compounds. Further assays are required to assess hydrolysates stability in food matrices and during digestion, and their effect on target sites.Este estágio permitiu a integração no projeto ValorMar, que visa melhorar indústrias de aquacultura e ao mesmo tempo fornecer benefícios de saúde ao homem. A aquacultura gera grandes quantidades de desperdício e subprodutos, o que constitui um problema económico, de saúde e ambiental. Estes subprodutos são conhecidos por possuírem potencial nutritivo e bioativo, merecedores de exploração para o fabrico de novos alimentos funcionais que possam exercer efeitos benéficos específicos na saúde e bem-estar humano. Deste modo, o objetivo do presente trabalho foi explorar o potencial bioativo do mexilhão Mediterrâneo (Mytilus galloprovincialis), um produto comum na aquacultura europeia e que gera altas quantidades de desperdício, através de hidrólise enzimática, para extrair compostos potencialmente bioativos que possam ser posteriormente aplicados na criação de novos alimentos funcionais. Homogeneizados de carne de mexilhão foram hidrolisados com Alcalase® e Protamex®, a um pH 7.5, temperatura de 60 °C, com diferentes concentrações de enzima (0, 1, 2 e 3%), de quantidade de substrato (25 e 50 g) e de duração (1 e 2 h). Os hidrolisados resultantes foram posteriormente processados através de liofilização ou ultrafiltração por Amicon®. Condições de pré-tratamento termal, hidrólise e processamento de hidrolisados foram avaliadas para compreender quais levariam à produção de péptidos bioativos. Os hidrolisados foram testados quanto à sua capacidade antioxidante, através do método de eliminação do radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), atividade antimicrobiana, pelas técnicas de difusão em disco e por poço, e atividade anti-hipertensiva pela sua capacidade em inibir a enzima conversora de angiotensina (iACE). Os hidrolisados produzidos revelaram que hidrólise com múltiplas enzimas produz um maior potencial bioativo. A capacidade de eliminar o radical DPPH aumentou com o substrato e mostrou ser inferior com a fração >3 kDa, ao contrário do potencial iACE, que aumentou com a fração ultrafiltrada. Em geral, hidrolisados de mexilhão fervido mostraram um alto potencial anti-hipertensivo. No entanto, nenhum efeito antimicrobiano foi observado nas amostras obtidas nas condições testadas. Estudos posteriores deverão ser realizados, ampliando condições de hidrólise e purificação e testando variados potenciais bioativos, de modo a reunir mais informação acerca do potencial desta espécie como fonte de compostos bioativos. Mais ensaios são requeridos para avaliar a estabilidade dos hidrolisados na matriz do alimento e durante digestão, e o seu efeito nos locais alvos.2023-03-02T00:00:00Z2021-02-17T00:00:00Z2021-02-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/30856engMoura, Maria Eduarda Nogueirainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:59:38Zoai:ria.ua.pt:10773/30856Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:02:51.116555Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing
title Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing
spellingShingle Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing
Moura, Maria Eduarda Nogueira
Alcalase
Antihypertensive activity
Antimicrobial activity
Antioxidant activity
Bioactive peptides
Enzymatic hydrolysis
Mytilus galloprovincialis
Protamex
title_short Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing
title_full Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing
title_fullStr Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing
title_full_unstemmed Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing
title_sort Bioactive potential of Mytilus galloprovincialis hydrolysates: effect of hydrolysis conditions and sample processing
author Moura, Maria Eduarda Nogueira
author_facet Moura, Maria Eduarda Nogueira
author_role author
dc.contributor.author.fl_str_mv Moura, Maria Eduarda Nogueira
dc.subject.por.fl_str_mv Alcalase
Antihypertensive activity
Antimicrobial activity
Antioxidant activity
Bioactive peptides
Enzymatic hydrolysis
Mytilus galloprovincialis
Protamex
topic Alcalase
Antihypertensive activity
Antimicrobial activity
Antioxidant activity
Bioactive peptides
Enzymatic hydrolysis
Mytilus galloprovincialis
Protamex
description This internship allowed integration in the ValorMar project, aimed at improving aquaculture industries while also providing health benefits to humans. Aquaculture generates great amounts of waste and by-products, which constitutes an economic, health and environmental problem. These by-products are known to possess nutritional and bioactive potential, worthy of exploration for the manufacture of novel functional foods, that can exert specific beneficial effects on human health and wellbeing. Therefore, the goal of the present work was to explore the bioactive potential of the Mediterranean mussel (Mytilus galloprovincialis), a common European aquaculture shellfish product, that generates high amounts of waste, through enzymatic hydrolysis, to extract potentially bioactive compounds that can later be applied in the creation of new functional foods. Mussel meat homogenates were subjected to hydrolysis with Alcalase® and Protamex®, at pH 7.5, temperature of 60 °C, with different enzyme concentrations (0, 1, 2 and 3%), substrate amounts (25 and 50 g) and duration (1 and 2h). Resultant hydrolysates were further processed through lyophilization or Amicon® ultrafiltration. Pre-thermal-treatment, hydrolysis and hydrolysates processing conditions were evaluated to understand which would lead to the production of bioactive peptides. Hydrolysates were tested for their antioxidant ability, through 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging method, antimicrobial activity, by disk and well diffusion techniques, and for antihypertensive ability by their capacity in inhibiting angiotensin-converting enzyme (iACE). Produced hydrolysates revealed that multiple enzyme hydrolysis produces higher bioactive potential. DPPH scavenging ability increased with substrate and revealed lower activity with the >3 kDa fraction, unlike iACE potential, which increased with the ultrafiltered fraction. In general, boiled mussel hydrolysates showed high antihypertensive potential. However, no antimicrobial effect was observed from samples obtained in the tested conditions. Further studies should be done, widening hydrolysis and purification conditions, testing for different bioactive potential, to gather more knowledge on this species potential as a source of bioactive compounds. Further assays are required to assess hydrolysates stability in food matrices and during digestion, and their effect on target sites.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-17T00:00:00Z
2021-02-17
2023-03-02T00:00:00Z
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