The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori.
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/2915 |
Resumo: | Helicobacter pylori is a pathogen that establishes long life infections responsible for chronic gastric ulcer diseases and a proved risk factor for gastric carcinoma. The therapeutic properties of carbon-monoxide releasing molecules (CORMs) led us to investigate their effect on H. pylori. We show that H. pylori 26695 is susceptible to two widely used CORMs, namely CORM-2 and CORM-3. Also, several H. pylori clinical isolates were killed by CORM-2, including those resistant to metronidazole. Moreover, sub-lethal doses of CORM-2 combined with metronidazole, amoxicillin and clarithromycin was found to potentiate the effect of the antibiotics. We further demonstrate that the mechanisms underpinning the antimicrobial effect of CORMs involve the inhibition of H. pylori respiration and urease activity. In vivo studies done in key cells of the innate immune system, such as macrophages, showed that CORM-2, either alone or when combined with metronidazole, strongly reduces the ability of H. pylori to infect animal cells. Hence, CORMs have the potential to kill antibiotic resistant strains of H. pylori. |
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The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori.Helicobacter pyloriCarbon Monoxide-releasing MoleculesInfecções GastrointestinaisHelicobacter pylori is a pathogen that establishes long life infections responsible for chronic gastric ulcer diseases and a proved risk factor for gastric carcinoma. The therapeutic properties of carbon-monoxide releasing molecules (CORMs) led us to investigate their effect on H. pylori. We show that H. pylori 26695 is susceptible to two widely used CORMs, namely CORM-2 and CORM-3. Also, several H. pylori clinical isolates were killed by CORM-2, including those resistant to metronidazole. Moreover, sub-lethal doses of CORM-2 combined with metronidazole, amoxicillin and clarithromycin was found to potentiate the effect of the antibiotics. We further demonstrate that the mechanisms underpinning the antimicrobial effect of CORMs involve the inhibition of H. pylori respiration and urease activity. In vivo studies done in key cells of the innate immune system, such as macrophages, showed that CORM-2, either alone or when combined with metronidazole, strongly reduces the ability of H. pylori to infect animal cells. Hence, CORMs have the potential to kill antibiotic resistant strains of H. pylori.Public Library of ScienceRepositório Científico do Instituto Nacional de SaúdeTavares, A.F.Parente, M.R.Justino, M.C.Oleastro, M.Nobre, L.S.Saraiva, L.M.2015-02-19T12:49:48Z2013-12-262013-12-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/2915engPLoS One. 2013 Dec 26;8(12):e83157. doi: 10.1371/journal.pone.0083157. eCollection 20131932-620310.1371/journal.pone.0083157info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:21Zoai:repositorio.insa.pt:10400.18/2915Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:37:33.554405Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. |
title |
The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. |
spellingShingle |
The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. Tavares, A.F. Helicobacter pylori Carbon Monoxide-releasing Molecules Infecções Gastrointestinais |
title_short |
The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. |
title_full |
The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. |
title_fullStr |
The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. |
title_full_unstemmed |
The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. |
title_sort |
The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. |
author |
Tavares, A.F. |
author_facet |
Tavares, A.F. Parente, M.R. Justino, M.C. Oleastro, M. Nobre, L.S. Saraiva, L.M. |
author_role |
author |
author2 |
Parente, M.R. Justino, M.C. Oleastro, M. Nobre, L.S. Saraiva, L.M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Tavares, A.F. Parente, M.R. Justino, M.C. Oleastro, M. Nobre, L.S. Saraiva, L.M. |
dc.subject.por.fl_str_mv |
Helicobacter pylori Carbon Monoxide-releasing Molecules Infecções Gastrointestinais |
topic |
Helicobacter pylori Carbon Monoxide-releasing Molecules Infecções Gastrointestinais |
description |
Helicobacter pylori is a pathogen that establishes long life infections responsible for chronic gastric ulcer diseases and a proved risk factor for gastric carcinoma. The therapeutic properties of carbon-monoxide releasing molecules (CORMs) led us to investigate their effect on H. pylori. We show that H. pylori 26695 is susceptible to two widely used CORMs, namely CORM-2 and CORM-3. Also, several H. pylori clinical isolates were killed by CORM-2, including those resistant to metronidazole. Moreover, sub-lethal doses of CORM-2 combined with metronidazole, amoxicillin and clarithromycin was found to potentiate the effect of the antibiotics. We further demonstrate that the mechanisms underpinning the antimicrobial effect of CORMs involve the inhibition of H. pylori respiration and urease activity. In vivo studies done in key cells of the innate immune system, such as macrophages, showed that CORM-2, either alone or when combined with metronidazole, strongly reduces the ability of H. pylori to infect animal cells. Hence, CORMs have the potential to kill antibiotic resistant strains of H. pylori. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-26 2013-12-26T00:00:00Z 2015-02-19T12:49:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/2915 |
url |
http://hdl.handle.net/10400.18/2915 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS One. 2013 Dec 26;8(12):e83157. doi: 10.1371/journal.pone.0083157. eCollection 2013 1932-6203 10.1371/journal.pone.0083157 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132109999177728 |