Exploring cytochrome-c’s biogenesis in eukaryotes

• Autor(a) principal: Raposo, Inês Tomàs de Aquino
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/114984
• topic: CCHL; cytochrome-c; Biochemistry

"C-type cytochromes are proteins ubiquitous to organisms from all domains of Life. They play essential roles in diverse processes, ranging from electron shuttles to participating in enzymatic active sites in organism’s metabolism and triggering apoptosis within cells. Contrary to other types of cytochromes, the haem cofactor is bound to the polypeptide chain of the holoprotein. For this process to occur, a maturation system capable of catalysing this reaction is required. Although these systems are well studied and characterised in other organisms, there is a clear lack of knowledge regarding System III, which is present in eukaryotes. System III is composed of a single protein, CCHL (in yeast) or HCCS (in humans). This makes it the simplest one, with CCHL being involved in both the import of the polypeptide and the covalent attachment of the haem to the polypeptide after recognition of a specific binding motif, CXXCH.(...)"