Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/19982 |
Resumo: | Mushrooms are important sources of natural bioactive compounds that are increasingly used as cosmeceutical ingredients. In this context, ethanolic extracts were prepared from Ganoderma lucidum and Pleurotus ostreatus and tested by incorporation into base cosmetic creams. In vitro safety evaluation of the extracts and cosmetic formulations prepared therefore was carried out using the MTT and LDH assays in keratinocyte (HaCaT) and fibroblast (HFF-1) cell lines. Moreover, they were submitted to ex vivo skin permeation studies using a Franz diffusion apparatus with pig ear skin as permeation membrane. The results showed the absence of toxicity for keratinocytes and fibroblasts in a concentration dependent manner, which is indicative of the safety of these extracts for cosmeceutical ingredients purposes. Protocatechuic and syringic acids were the only compounds permeating from G. lucidum extract in the first 8 h of study, no penetration was observed for phenolic acids found in P. ostreatus extract and formulation. These results pointed out for the suitability of using mushroom extracts as skin care ingredients and may contribute for the valorisation of wastes generated by the mushroom processing industry, which can serve as raw-materials for the obtainment of the extracts. |
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Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studiesBioactive compoundsCosmeceuticalsCytotoxicityMushroomsSkin permeationMushrooms are important sources of natural bioactive compounds that are increasingly used as cosmeceutical ingredients. In this context, ethanolic extracts were prepared from Ganoderma lucidum and Pleurotus ostreatus and tested by incorporation into base cosmetic creams. In vitro safety evaluation of the extracts and cosmetic formulations prepared therefore was carried out using the MTT and LDH assays in keratinocyte (HaCaT) and fibroblast (HFF-1) cell lines. Moreover, they were submitted to ex vivo skin permeation studies using a Franz diffusion apparatus with pig ear skin as permeation membrane. The results showed the absence of toxicity for keratinocytes and fibroblasts in a concentration dependent manner, which is indicative of the safety of these extracts for cosmeceutical ingredients purposes. Protocatechuic and syringic acids were the only compounds permeating from G. lucidum extract in the first 8 h of study, no penetration was observed for phenolic acids found in P. ostreatus extract and formulation. These results pointed out for the suitability of using mushroom extracts as skin care ingredients and may contribute for the valorisation of wastes generated by the mushroom processing industry, which can serve as raw-materials for the obtainment of the extracts.The authors are grateful to the Foundation for Science and Technology and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019) and Associate Laboratory LSRE-LCM (UID/EQU/50020/2019) funded by national funds through FCT/ MCTES (PIDDAC). This work is funded by the European Agricultural Fund for Rural Development (EAFRD), through the Rural Development Program (PDR2020), within the scope of Project MicoCoating (PDR2020-101-031472). Francisca Rodrigues is thankful for her postdoc research grant from the project Operação NORTE-01-0145- FEDER- 000011. This work received financial support from the European Union (FEDER funds through COMPETE), under the Partnership Agreement PT 2020, and National Funds (FCT, Foundation for Science and Technology) through project LAQV/UID/QUI/50006/ 2013 and NORTE-07-0124-FEDER-000069 – Food Science.Biblioteca Digital do IPBTaofiq, OludemiRodrigues, FranciscaBarros, LillianBarreiro, M.F.Ferreira, Isabel C.F.R.Oliveira, Beatriz2018-01-19T10:00:00Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/19982engTaofiq, Oludemi; Rodrigues, Francisca; Barros, Lillian; Barreiro, Maria F.; Ferreira, Isabel C.F.R.; Oliveira, M. Beatriz P.P. (2019). Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies. Food and Chemical Toxicology. ISSN 0278-6915. 127, p. 228-2360278-691510.1016/j.fct.2019.03.045info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:45:48Zoai:bibliotecadigital.ipb.pt:10198/19982Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:10:47.707735Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies |
title |
Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies |
spellingShingle |
Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies Taofiq, Oludemi Bioactive compounds Cosmeceuticals Cytotoxicity Mushrooms Skin permeation |
title_short |
Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies |
title_full |
Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies |
title_fullStr |
Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies |
title_full_unstemmed |
Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies |
title_sort |
Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies |
author |
Taofiq, Oludemi |
author_facet |
Taofiq, Oludemi Rodrigues, Francisca Barros, Lillian Barreiro, M.F. Ferreira, Isabel C.F.R. Oliveira, Beatriz |
author_role |
author |
author2 |
Rodrigues, Francisca Barros, Lillian Barreiro, M.F. Ferreira, Isabel C.F.R. Oliveira, Beatriz |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Taofiq, Oludemi Rodrigues, Francisca Barros, Lillian Barreiro, M.F. Ferreira, Isabel C.F.R. Oliveira, Beatriz |
dc.subject.por.fl_str_mv |
Bioactive compounds Cosmeceuticals Cytotoxicity Mushrooms Skin permeation |
topic |
Bioactive compounds Cosmeceuticals Cytotoxicity Mushrooms Skin permeation |
description |
Mushrooms are important sources of natural bioactive compounds that are increasingly used as cosmeceutical ingredients. In this context, ethanolic extracts were prepared from Ganoderma lucidum and Pleurotus ostreatus and tested by incorporation into base cosmetic creams. In vitro safety evaluation of the extracts and cosmetic formulations prepared therefore was carried out using the MTT and LDH assays in keratinocyte (HaCaT) and fibroblast (HFF-1) cell lines. Moreover, they were submitted to ex vivo skin permeation studies using a Franz diffusion apparatus with pig ear skin as permeation membrane. The results showed the absence of toxicity for keratinocytes and fibroblasts in a concentration dependent manner, which is indicative of the safety of these extracts for cosmeceutical ingredients purposes. Protocatechuic and syringic acids were the only compounds permeating from G. lucidum extract in the first 8 h of study, no penetration was observed for phenolic acids found in P. ostreatus extract and formulation. These results pointed out for the suitability of using mushroom extracts as skin care ingredients and may contribute for the valorisation of wastes generated by the mushroom processing industry, which can serve as raw-materials for the obtainment of the extracts. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/19982 |
url |
http://hdl.handle.net/10198/19982 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Taofiq, Oludemi; Rodrigues, Francisca; Barros, Lillian; Barreiro, Maria F.; Ferreira, Isabel C.F.R.; Oliveira, M. Beatriz P.P. (2019). Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies. Food and Chemical Toxicology. ISSN 0278-6915. 127, p. 228-236 0278-6915 10.1016/j.fct.2019.03.045 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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