Drinking water biofilm assessment of total and culturable bacteria under different operating conditions
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/4852 |
Resumo: | Monitoring of biofilms subjected to different operating conditions was performed using a flow cell system. The system was fed by chlorine-free tap water, with and without added nutrients (0.5 mg l ˉ¹carbon, 0.1 mg l ˉ¹nitrogen and 0.01 mg lˉ¹ phosphorus), and biofilms were grown on polyvinyl chloride (PVC) and stainless steel (SS) coupons, both in laminar and turbulent flow. The parameters analysed were culturable cells, using R2A, and total bacteria, which was assessed using the 4,6-diamino-2-phenylindole (DAPI) staining method. The impact of the different operating conditions in the studied parameters was established using Multivariate Analysis of Variance (MANOVA). From the most relevant to the least relevant factor, the total and culturable bacteria in biofilms increased due to the addition of nutrients to water (F=20.005; p<0.001); the use of turbulent (Re=11000) instead of laminar (Re=2000) hydrodynamic flows (F=9.173; p<0.001); and the use of PVC instead of SS as the support material (F=2.848; p=0.060). Interactions between these conditions, namely between surface and flow (F=8.235; p<0.001) and also flow and nutrients (F=5.498; p<0.05) have also proved to significantly influence biofilm formation. This work highlights the need for a deeper understanding of how the large spectrum of conditions interact and affect biofilm formation potential and accumulation with the final purpose of predicting the total and culturable bacteria attached to real drinking water distribution pipes based on the system characteristics. |
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Drinking water biofilm assessment of total and culturable bacteria under different operating conditionsBiofilmDrinking waterNutrient contentFlow rateSupport materialScience & TechnologyMonitoring of biofilms subjected to different operating conditions was performed using a flow cell system. The system was fed by chlorine-free tap water, with and without added nutrients (0.5 mg l ˉ¹carbon, 0.1 mg l ˉ¹nitrogen and 0.01 mg lˉ¹ phosphorus), and biofilms were grown on polyvinyl chloride (PVC) and stainless steel (SS) coupons, both in laminar and turbulent flow. The parameters analysed were culturable cells, using R2A, and total bacteria, which was assessed using the 4,6-diamino-2-phenylindole (DAPI) staining method. The impact of the different operating conditions in the studied parameters was established using Multivariate Analysis of Variance (MANOVA). From the most relevant to the least relevant factor, the total and culturable bacteria in biofilms increased due to the addition of nutrients to water (F=20.005; p<0.001); the use of turbulent (Re=11000) instead of laminar (Re=2000) hydrodynamic flows (F=9.173; p<0.001); and the use of PVC instead of SS as the support material (F=2.848; p=0.060). Interactions between these conditions, namely between surface and flow (F=8.235; p<0.001) and also flow and nutrients (F=5.498; p<0.05) have also proved to significantly influence biofilm formation. This work highlights the need for a deeper understanding of how the large spectrum of conditions interact and affect biofilm formation potential and accumulation with the final purpose of predicting the total and culturable bacteria attached to real drinking water distribution pipes based on the system characteristics.Fundação para a Ciência e a Tecnologia (FCT); European Commission Research Project SAFER.Taylor and FrancisUniversidade do MinhoSimões, Lúcia C.Azevedo, N. F.Pacheco, A. P.Keevil, C. W.Vieira, M. J.20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/4852eng"Biofouling". ISSN 0892-7014. 22:2 (2006) 91-99.0892-701410.1080/0892701060059860316581673info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:41:44ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Drinking water biofilm assessment of total and culturable bacteria under different operating conditions |
title |
Drinking water biofilm assessment of total and culturable bacteria under different operating conditions |
spellingShingle |
Drinking water biofilm assessment of total and culturable bacteria under different operating conditions Simões, Lúcia C. Biofilm Drinking water Nutrient content Flow rate Support material Science & Technology |
title_short |
Drinking water biofilm assessment of total and culturable bacteria under different operating conditions |
title_full |
Drinking water biofilm assessment of total and culturable bacteria under different operating conditions |
title_fullStr |
Drinking water biofilm assessment of total and culturable bacteria under different operating conditions |
title_full_unstemmed |
Drinking water biofilm assessment of total and culturable bacteria under different operating conditions |
title_sort |
Drinking water biofilm assessment of total and culturable bacteria under different operating conditions |
author |
Simões, Lúcia C. |
author_facet |
Simões, Lúcia C. Azevedo, N. F. Pacheco, A. P. Keevil, C. W. Vieira, M. J. |
author_role |
author |
author2 |
Azevedo, N. F. Pacheco, A. P. Keevil, C. W. Vieira, M. J. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Simões, Lúcia C. Azevedo, N. F. Pacheco, A. P. Keevil, C. W. Vieira, M. J. |
dc.subject.por.fl_str_mv |
Biofilm Drinking water Nutrient content Flow rate Support material Science & Technology |
topic |
Biofilm Drinking water Nutrient content Flow rate Support material Science & Technology |
description |
Monitoring of biofilms subjected to different operating conditions was performed using a flow cell system. The system was fed by chlorine-free tap water, with and without added nutrients (0.5 mg l ˉ¹carbon, 0.1 mg l ˉ¹nitrogen and 0.01 mg lˉ¹ phosphorus), and biofilms were grown on polyvinyl chloride (PVC) and stainless steel (SS) coupons, both in laminar and turbulent flow. The parameters analysed were culturable cells, using R2A, and total bacteria, which was assessed using the 4,6-diamino-2-phenylindole (DAPI) staining method. The impact of the different operating conditions in the studied parameters was established using Multivariate Analysis of Variance (MANOVA). From the most relevant to the least relevant factor, the total and culturable bacteria in biofilms increased due to the addition of nutrients to water (F=20.005; p<0.001); the use of turbulent (Re=11000) instead of laminar (Re=2000) hydrodynamic flows (F=9.173; p<0.001); and the use of PVC instead of SS as the support material (F=2.848; p=0.060). Interactions between these conditions, namely between surface and flow (F=8.235; p<0.001) and also flow and nutrients (F=5.498; p<0.05) have also proved to significantly influence biofilm formation. This work highlights the need for a deeper understanding of how the large spectrum of conditions interact and affect biofilm formation potential and accumulation with the final purpose of predicting the total and culturable bacteria attached to real drinking water distribution pipes based on the system characteristics. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2006-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/4852 |
url |
http://hdl.handle.net/1822/4852 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Biofouling". ISSN 0892-7014. 22:2 (2006) 91-99. 0892-7014 10.1080/08927010600598603 16581673 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Taylor and Francis |
publisher.none.fl_str_mv |
Taylor and Francis |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
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repository.mail.fl_str_mv |
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_version_ |
1777303820175409152 |