Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa

Bibliographic Details
Main Author: Rabna, Paulo
Publication Date: 2015
Other Authors: Ramos, Jorge, Ponce, Gema, Sanca, Lilica, Mané, Morto, Armada, Ana, Machado, Diana, Vieira, Fina, Gomes, Victor F., Martins, Elisabete, Colombatti, Raffaella, Riccardi, Fabio, Perdigão, João, Sotero, Joana, Portugal, Isabel, Couto, Isabel, Atouguia, Jorge, Rodrigues, Amabélia, Viveiros, Miguel
Format: Article
Language: eng
Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Download full: https://doi.org/10.1371/journal.pone.0127536
Summary: PMID:26017968 WOS:000355185600088
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spelling Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West AfricaBiochemistry, Genetics and Molecular Biology (miscellaneous)Infectious DiseasesEpidemiologySDG 3 - Good Health and Well-beingSDG 10 - Reduced InequalitiesPMID:26017968 WOS:000355185600088Background This study aimed to evaluate the usefulness of the Xpert MTB/RIF assay for the rapid direct detection of M. tuberculosis complex (MTBC) strains and rifampicin resistance associated mutations in a resource-limited setting such as Guinea-Bissau and its implications in the management of tuberculosis (TB) and drug resistant tuberculosis, complementing the scarce information on resistance and genotypic diversity of MTBC strains in this West African country. Methods and Results This cross-sectional prospective study included 100 consecutive TB patients with positive acid-fast smears at two months of anti-tuberculosis treatment or in a re-treatment situation, between May and December 2012. Resistance to rifampicin was detected using the GeneXpert system and the Xpert MTB/RIF assay. MTBC isolates obtained with the BACTEC MGIT 960 system were tested for susceptibility to first- And second-line anti-tuberculosis drugs. Overall, the prevalence of multidrug-resistant tuberculosis (MDR-TB) was found to be 9 cases. Of these, 67% (6 patients) of confirmed MDR-TB cases had no past history of TB treatment and 33% (3 patients) were previously treated cases. Extensively drug-resistant TB was not found. Molecular typing of the MDR-TB strains revealed recent transmission patterns of imported MDR strains. Conclusions The Xpert MTB/RIF assay was reliable for the detection of rifampicin resistant MTBC strains directly from sputum samples of patients undergoing first-line treatment for two months, being more trustworthy than the simple presence of acid-fast bacilli in the smear. Its implementation is technically simple, does not require specialized laboratory infrastructures and is suitable for resource-limited settings when a regular source of electricity and maintenance is available as well as financial and operation sustainability is guaranteed by the health authorities. A high prevalence of MDR-TB among patients at risk of MDR-TB after two months of first-line treatment was found, in support of the WHO recommendations for its use in the management of this risk group.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)TB, HIV and opportunistic diseases and pathogens (THOP)Vector borne diseases and pathogens (VBD)RUNRabna, PauloRamos, JorgePonce, GemaSanca, LilicaMané, MortoArmada, AnaMachado, DianaVieira, FinaGomes, Victor F.Martins, ElisabeteColombatti, RaffaellaRiccardi, FabioPerdigão, JoãoSotero, JoanaPortugal, IsabelCouto, IsabelAtouguia, JorgeRodrigues, AmabéliaViveiros, Miguel2018-05-14T22:02:18Z2015-05-012015-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1371/journal.pone.0127536eng1932-6203PURE: 1731242http://www.scopus.com/inward/record.url?scp=84958198245&partnerID=8YFLogxKhttps://doi.org/10.1371/journal.pone.0127536info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:30Zoai:run.unl.pt:10362/36943Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:43.119612Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
title Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
spellingShingle Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
Rabna, Paulo
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Infectious Diseases
Epidemiology
SDG 3 - Good Health and Well-being
SDG 10 - Reduced Inequalities
title_short Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
title_full Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
title_fullStr Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
title_full_unstemmed Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
title_sort Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
author Rabna, Paulo
author_facet Rabna, Paulo
Ramos, Jorge
Ponce, Gema
Sanca, Lilica
Mané, Morto
Armada, Ana
Machado, Diana
Vieira, Fina
Gomes, Victor F.
Martins, Elisabete
Colombatti, Raffaella
Riccardi, Fabio
Perdigão, João
Sotero, Joana
Portugal, Isabel
Couto, Isabel
Atouguia, Jorge
Rodrigues, Amabélia
Viveiros, Miguel
author_role author
author2 Ramos, Jorge
Ponce, Gema
Sanca, Lilica
Mané, Morto
Armada, Ana
Machado, Diana
Vieira, Fina
Gomes, Victor F.
Martins, Elisabete
Colombatti, Raffaella
Riccardi, Fabio
Perdigão, João
Sotero, Joana
Portugal, Isabel
Couto, Isabel
Atouguia, Jorge
Rodrigues, Amabélia
Viveiros, Miguel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
TB, HIV and opportunistic diseases and pathogens (THOP)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv Rabna, Paulo
Ramos, Jorge
Ponce, Gema
Sanca, Lilica
Mané, Morto
Armada, Ana
Machado, Diana
Vieira, Fina
Gomes, Victor F.
Martins, Elisabete
Colombatti, Raffaella
Riccardi, Fabio
Perdigão, João
Sotero, Joana
Portugal, Isabel
Couto, Isabel
Atouguia, Jorge
Rodrigues, Amabélia
Viveiros, Miguel
dc.subject.por.fl_str_mv Biochemistry, Genetics and Molecular Biology (miscellaneous)
Infectious Diseases
Epidemiology
SDG 3 - Good Health and Well-being
SDG 10 - Reduced Inequalities
topic Biochemistry, Genetics and Molecular Biology (miscellaneous)
Infectious Diseases
Epidemiology
SDG 3 - Good Health and Well-being
SDG 10 - Reduced Inequalities
description PMID:26017968 WOS:000355185600088
publishDate 2015
dc.date.none.fl_str_mv 2015-05-01
2015-05-01T00:00:00Z
2018-05-14T22:02:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1371/journal.pone.0127536
url https://doi.org/10.1371/journal.pone.0127536
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 1731242
http://www.scopus.com/inward/record.url?scp=84958198245&partnerID=8YFLogxK
https://doi.org/10.1371/journal.pone.0127536
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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