Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal

Detalhes bibliográficos
Autor(a) principal: Carneiro, Sofia
Data de Publicação: 2023
Outros Autores: Pinto, Miguel, Silva, Sónia, Santos, Andrea, Rodrigues, Irene, Santos, Daniela, Duarte, Sílvia, Vieira, Luís, Gomes, João Paulo, Macedo, Rita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/9087
Resumo: (This article belongs to the Special Issue Advances in Molecular Biology on Mycobacteria)
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spelling Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from PortugalMABCMycobacterium abscessusPortugalAntimicrobial ResistanceGenomic DiversityNontuberculous MycobacteriawgMLSTwhole-genome SequencingResistência aos Antimicrobianos(This article belongs to the Special Issue Advances in Molecular Biology on Mycobacteria)The Mycobacterium abscessus complex (MABC) is an emerging, difficult to treat, multidrug-resistant nontuberculous mycobacteria responsible for a wide spectrum of infections and associated with an increasing number of cases worldwide. Dominant circulating clones (DCCs) of MABC have been genetically identified as groups of strains associated with higher prevalence, higher levels of antimicrobial resistance, and worse clinical outcomes. To date, little is known about the genomic characteristics of MABC species circulating in Portugal. Here, we examined the genetic diversity and antimicrobial resistance profiles of 30 MABC strains isolated between 2014 and 2022 in Portugal. The genetic diversity of circulating MABC strains was assessed through a gene-by-gene approach (wgMLST), allowing their subspecies differentiation and the classification of isolates into DCCs. Antimicrobial resistance profiles were defined using phenotypic, molecular, and genomic approaches. The majority of isolates were resistant to at least two antimicrobials, although a poor correlation between phenotype and genotype data was observed. Portuguese genomes were highly diverse, and data suggest the existence of MABC lineages with potential international circulation or cross-border transmission. This study highlights the genetic diversity and antimicrobial resistance profile of circulating MABC isolates in Portugal while representing the first step towards the implementation of a genomic-based surveillance system for MABC at the Portuguese NIH.Funding: The acquisition of equipment for whole-genome sequencing used in this study was funded by the HERA (Human and Environmental Risk Assessment) project (Grant/2021/PHF/23776) supported by the European Commission through the European Centre for Disease Prevention and Control (ECDC). This work was also partially funded by the GenomePT project (POCI-01-0145-FEDER-022184) supported by COMPETE 2020 (Operational Programme for Competitiveness and Internationalisation (POCI)), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF), and the Portuguese Science and Technology Foundation (FCT). SC was supported by a PhD fellowship from the FCT (2020.08503.BD).MDPIRepositório Científico do Instituto Nacional de SaúdeCarneiro, SofiaPinto, MiguelSilva, SóniaSantos, AndreaRodrigues, IreneSantos, DanielaDuarte, SílviaVieira, LuísGomes, João PauloMacedo, Rita2024-02-12T15:24:23Z2023-10-202023-10-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/9087engInt J Mol Sci. 2023 Oct 20;24(20):15402. doi: 10.3390/ijms2420154021661-659610.3390/ijms242015402info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-17T01:31:21Zoai:repositorio.insa.pt:10400.18/9087Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:38:34.297204Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
spellingShingle Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
Carneiro, Sofia
MABC
Mycobacterium abscessus
Portugal
Antimicrobial Resistance
Genomic Diversity
Nontuberculous Mycobacteria
wgMLST
whole-genome Sequencing
Resistência aos Antimicrobianos
title_short Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title_full Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title_fullStr Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title_full_unstemmed Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title_sort Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
author Carneiro, Sofia
author_facet Carneiro, Sofia
Pinto, Miguel
Silva, Sónia
Santos, Andrea
Rodrigues, Irene
Santos, Daniela
Duarte, Sílvia
Vieira, Luís
Gomes, João Paulo
Macedo, Rita
author_role author
author2 Pinto, Miguel
Silva, Sónia
Santos, Andrea
Rodrigues, Irene
Santos, Daniela
Duarte, Sílvia
Vieira, Luís
Gomes, João Paulo
Macedo, Rita
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Carneiro, Sofia
Pinto, Miguel
Silva, Sónia
Santos, Andrea
Rodrigues, Irene
Santos, Daniela
Duarte, Sílvia
Vieira, Luís
Gomes, João Paulo
Macedo, Rita
dc.subject.por.fl_str_mv MABC
Mycobacterium abscessus
Portugal
Antimicrobial Resistance
Genomic Diversity
Nontuberculous Mycobacteria
wgMLST
whole-genome Sequencing
Resistência aos Antimicrobianos
topic MABC
Mycobacterium abscessus
Portugal
Antimicrobial Resistance
Genomic Diversity
Nontuberculous Mycobacteria
wgMLST
whole-genome Sequencing
Resistência aos Antimicrobianos
description (This article belongs to the Special Issue Advances in Molecular Biology on Mycobacteria)
publishDate 2023
dc.date.none.fl_str_mv 2023-10-20
2023-10-20T00:00:00Z
2024-02-12T15:24:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/9087
url http://hdl.handle.net/10400.18/9087
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Mol Sci. 2023 Oct 20;24(20):15402. doi: 10.3390/ijms242015402
1661-6596
10.3390/ijms242015402
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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