Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/22085 |
Resumo: | Nowadays skin prick tests remain the favourite methodology in allergy diagnosis to aeroallergens. These tests, however, cause discomfort to the patient. Several biochemical methods are available but have limited diagnostic power as biological response, hence allergic reaction, is not predicted by these tests. A biological assay allowing the evaluation of allergic response to allergens is lacking. The aim of this work was to investigate the value of a rat basophil cell line expressing human high affinity IgE receptor (FcERI) for the evaluation of specific response to allergens. FACS analysis was used to determine FcERI expression (APC-A). Pollen extracts from different species were prepared with ammonium bicarbonate buffer, lyophilized and stored at -80ºC until use. Batches of RBL-h21 cells were sensitized with selected pools of human sera (purified IgE was used as control) and were stimulated with pollen extracts or Anti-IgE antibody. Histamine release (% degranulation) was determined a ß-hesoaminidase (tracer) assay. Batches of RBL-h21 cells sensitized (human sera) to grasses (D. glomerata), olive, oak or plane exhibited selective and dose-dependent degranulation upon selective stimulation with pollen extracts in the range of 1-200μg/mL. Maximal degranulation (>25%) was observed for 50, 120 and 62μg/mL for grass, oak and plane, respectively, while the lowest concentration with observed effect was 12μg/mL for any of the extracts. The results show that RBL-h21 cells sensitized with human sera exhibit specific and dose-dependent degranulation upon stimulation with pollen extract containing allergens suggesting this bioassay may constitute a useful tool to evaluate allergic responses both for research and/or diagnostics. |
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Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI.RBLbasophilFcERI (RBL-h21)allergyNowadays skin prick tests remain the favourite methodology in allergy diagnosis to aeroallergens. These tests, however, cause discomfort to the patient. Several biochemical methods are available but have limited diagnostic power as biological response, hence allergic reaction, is not predicted by these tests. A biological assay allowing the evaluation of allergic response to allergens is lacking. The aim of this work was to investigate the value of a rat basophil cell line expressing human high affinity IgE receptor (FcERI) for the evaluation of specific response to allergens. FACS analysis was used to determine FcERI expression (APC-A). Pollen extracts from different species were prepared with ammonium bicarbonate buffer, lyophilized and stored at -80ºC until use. Batches of RBL-h21 cells were sensitized with selected pools of human sera (purified IgE was used as control) and were stimulated with pollen extracts or Anti-IgE antibody. Histamine release (% degranulation) was determined a ß-hesoaminidase (tracer) assay. Batches of RBL-h21 cells sensitized (human sera) to grasses (D. glomerata), olive, oak or plane exhibited selective and dose-dependent degranulation upon selective stimulation with pollen extracts in the range of 1-200μg/mL. Maximal degranulation (>25%) was observed for 50, 120 and 62μg/mL for grass, oak and plane, respectively, while the lowest concentration with observed effect was 12μg/mL for any of the extracts. The results show that RBL-h21 cells sensitized with human sera exhibit specific and dose-dependent degranulation upon stimulation with pollen extract containing allergens suggesting this bioassay may constitute a useful tool to evaluate allergic responses both for research and/or diagnostics.European Academy of Allergy and Clinical Immunology2018-02-08T11:41:47Z2018-02-082017-06-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/22085http://hdl.handle.net/10174/22085porAntunes CM, Candeias J, Arriegas R, Tarana I, Galveias A, Martins LM, Bento OP, Lopes L, Costa A, Buters J. Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. EAACI 2017, TPS 03, P620. EAACI Congress 2017. Helsínquia, Finlândia, 17 a 21 de junho de 2017.http://react-profile.org/ebook/EAACI2017_FinalProgramme/#118/znaonaosimICTcmma@uevora.ptjoanc@hotmail.comd36818@alunos.uevora.ptinesbtarana@hotmail.comanagalveias@gmail.comlmlm@uevora.ptofelia@uevora.ptluisalopes@sapo.ptacrc@uevora.ptbuters@tum.de365DOI: 10.13140/RG.2.2.27328.02561Antunes, CéliaCandeias, JoanaArriegas, RuteTarana, InêsGalveias, AnaMartins, LuísBento, OféliaLopes, LuísaCosta, AnaButers, Jeroeninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:13:19Zoai:dspace.uevora.pt:10174/22085Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:13:15.824353Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. |
title |
Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. |
spellingShingle |
Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. Antunes, Célia RBL basophil FcERI (RBL-h21) allergy |
title_short |
Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. |
title_full |
Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. |
title_fullStr |
Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. |
title_full_unstemmed |
Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. |
title_sort |
Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. |
author |
Antunes, Célia |
author_facet |
Antunes, Célia Candeias, Joana Arriegas, Rute Tarana, Inês Galveias, Ana Martins, Luís Bento, Ofélia Lopes, Luísa Costa, Ana Buters, Jeroen |
author_role |
author |
author2 |
Candeias, Joana Arriegas, Rute Tarana, Inês Galveias, Ana Martins, Luís Bento, Ofélia Lopes, Luísa Costa, Ana Buters, Jeroen |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Antunes, Célia Candeias, Joana Arriegas, Rute Tarana, Inês Galveias, Ana Martins, Luís Bento, Ofélia Lopes, Luísa Costa, Ana Buters, Jeroen |
dc.subject.por.fl_str_mv |
RBL basophil FcERI (RBL-h21) allergy |
topic |
RBL basophil FcERI (RBL-h21) allergy |
description |
Nowadays skin prick tests remain the favourite methodology in allergy diagnosis to aeroallergens. These tests, however, cause discomfort to the patient. Several biochemical methods are available but have limited diagnostic power as biological response, hence allergic reaction, is not predicted by these tests. A biological assay allowing the evaluation of allergic response to allergens is lacking. The aim of this work was to investigate the value of a rat basophil cell line expressing human high affinity IgE receptor (FcERI) for the evaluation of specific response to allergens. FACS analysis was used to determine FcERI expression (APC-A). Pollen extracts from different species were prepared with ammonium bicarbonate buffer, lyophilized and stored at -80ºC until use. Batches of RBL-h21 cells were sensitized with selected pools of human sera (purified IgE was used as control) and were stimulated with pollen extracts or Anti-IgE antibody. Histamine release (% degranulation) was determined a ß-hesoaminidase (tracer) assay. Batches of RBL-h21 cells sensitized (human sera) to grasses (D. glomerata), olive, oak or plane exhibited selective and dose-dependent degranulation upon selective stimulation with pollen extracts in the range of 1-200μg/mL. Maximal degranulation (>25%) was observed for 50, 120 and 62μg/mL for grass, oak and plane, respectively, while the lowest concentration with observed effect was 12μg/mL for any of the extracts. The results show that RBL-h21 cells sensitized with human sera exhibit specific and dose-dependent degranulation upon stimulation with pollen extract containing allergens suggesting this bioassay may constitute a useful tool to evaluate allergic responses both for research and/or diagnostics. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-18T00:00:00Z 2018-02-08T11:41:47Z 2018-02-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/22085 http://hdl.handle.net/10174/22085 |
url |
http://hdl.handle.net/10174/22085 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Antunes CM, Candeias J, Arriegas R, Tarana I, Galveias A, Martins LM, Bento OP, Lopes L, Costa A, Buters J. Q. rotundifolia and P. hybrida pollen extracts induced basophil degranulation: study using a cell line expressing human FcERI. EAACI 2017, TPS 03, P620. EAACI Congress 2017. Helsínquia, Finlândia, 17 a 21 de junho de 2017. http://react-profile.org/ebook/EAACI2017_FinalProgramme/#118/z nao nao sim ICT cmma@uevora.pt joanc@hotmail.com d36818@alunos.uevora.pt inesbtarana@hotmail.com anagalveias@gmail.com lmlm@uevora.pt ofelia@uevora.pt luisalopes@sapo.pt acrc@uevora.pt buters@tum.de 365 DOI: 10.13140/RG.2.2.27328.02561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
European Academy of Allergy and Clinical Immunology |
publisher.none.fl_str_mv |
European Academy of Allergy and Clinical Immunology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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