Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems

Detalhes bibliográficos
Autor(a) principal: Silva, N. M.
Data de Publicação: 2015
Outros Autores: Ferreira, L., Madeira, Pedro P., Teixeira, J. A., Uversky, Vladimir N., Zaslavsky, Boris Y.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/39513
Resumo: Abstract Partition behavior of eight small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.215 M NaCl and 0.5 M osmolyte (sorbitol, sucrose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate-0.215 M NaCl system, all in 0.01 M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. The partition coefficients of all compounds examined (including proteins) were described in terms of solute-solvent interactions. The results obtained in the study show that solute-solvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system change in the presence of NaCl additive.
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spelling Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systemsAqueous two-phase systemPartitioningProteinsSolute-water interactionsSolvatochromic comparison methodSolvent propertiesScience & TechnologyAbstract Partition behavior of eight small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.215 M NaCl and 0.5 M osmolyte (sorbitol, sucrose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate-0.215 M NaCl system, all in 0.01 M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. The partition coefficients of all compounds examined (including proteins) were described in terms of solute-solvent interactions. The results obtained in the study show that solute-solvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system change in the presence of NaCl additive.Nuno da Silva acknowledges the financial support by Fundação para a Ciência e a Tecnologia (FCT) of the Portuguese’s Ministry for Science, Technology and Higher Education, in the framework of the Operational Program COMPETE (PTDC/EQU-FTT/120332/2010) and Fundação para a Ciência e Tecnologia the strategic funding of UID/BIO/04469/2013.Pedro P. Madeira acknowledges the financial support in part provided by (i) FCT/MEC, FEDER under Program PT2020 (Project UID/EQU/50020/2013) and (ii) QREN, ON2 and FEDER, under Program COMPETE (Project NORTE-07-0124-FEDER-0000011).ElsevierUniversidade do MinhoSilva, N. M.Ferreira, L.Madeira, Pedro P.Teixeira, J. A.Uversky, Vladimir N.Zaslavsky, Boris Y.2015-12-182015-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/39513engSilva, N. M.; Ferreira, L.; Madeira, Pedro P.; Teixeira, J. A.; Uversky, Vladimir N.; Zaslavsky, Boris Y., Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems. Journal of Chromatography A, 1425, 51-61, 20150021-96730021-967310.1016/j.chroma.2015.11.01926615710http://www.journals.elsevier.com/journal-of-chromatography-ainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:16Zoai:repositorium.sdum.uminho.pt:1822/39513Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:28.885953Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
title Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
spellingShingle Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
Silva, N. M.
Aqueous two-phase system
Partitioning
Proteins
Solute-water interactions
Solvatochromic comparison method
Solvent properties
Science & Technology
title_short Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
title_full Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
title_fullStr Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
title_full_unstemmed Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
title_sort Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
author Silva, N. M.
author_facet Silva, N. M.
Ferreira, L.
Madeira, Pedro P.
Teixeira, J. A.
Uversky, Vladimir N.
Zaslavsky, Boris Y.
author_role author
author2 Ferreira, L.
Madeira, Pedro P.
Teixeira, J. A.
Uversky, Vladimir N.
Zaslavsky, Boris Y.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, N. M.
Ferreira, L.
Madeira, Pedro P.
Teixeira, J. A.
Uversky, Vladimir N.
Zaslavsky, Boris Y.
dc.subject.por.fl_str_mv Aqueous two-phase system
Partitioning
Proteins
Solute-water interactions
Solvatochromic comparison method
Solvent properties
Science & Technology
topic Aqueous two-phase system
Partitioning
Proteins
Solute-water interactions
Solvatochromic comparison method
Solvent properties
Science & Technology
description Abstract Partition behavior of eight small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.215 M NaCl and 0.5 M osmolyte (sorbitol, sucrose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate-0.215 M NaCl system, all in 0.01 M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. The partition coefficients of all compounds examined (including proteins) were described in terms of solute-solvent interactions. The results obtained in the study show that solute-solvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system change in the presence of NaCl additive.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-18
2015-12-18T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/39513
url http://hdl.handle.net/1822/39513
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, N. M.; Ferreira, L.; Madeira, Pedro P.; Teixeira, J. A.; Uversky, Vladimir N.; Zaslavsky, Boris Y., Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems. Journal of Chromatography A, 1425, 51-61, 2015
0021-9673
0021-9673
10.1016/j.chroma.2015.11.019
26615710
http://www.journals.elsevier.com/journal-of-chromatography-a
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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