Mycorrhiza-like structures in rooted microshoots of Pinus pinea L.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/4065 |
Resumo: | Pinus pinea L. (stone pine) is one of the major plantation species in Iberian Peninsula, being Portugal the largest edible seed producer in the world. The induction and improvement of in vitro rhizogenesis of microshoots of Pinus pinea was developed in our laboratory using a co-culture system with ECM fungi. In the acclimation phase in mixed substrates, or in rhizotrons, anatomical and morphological studies were done to observe the evolution of the root system in microshoots from the co-culture system vs. control plants. Extensive dichotomous and coralloid branching of lateral roots occurred spontaneously in inoculated and control plants as well. Moreover, similar branching occurred in liquid culture of excised seedling roots without the presence of ECM fungi. The striking similarity of these organs with pine ectomycorrhizas prompted their anatomical analysis; however the presence of Hartig net was not confirmed. These results suggested that the development of ECM-like structures might have occurred spontaneously. |
id |
RCAP_9767904f6bf1885c05146b71189f805b |
---|---|
oai_identifier_str |
oai:digituma.uma.pt:10400.13/4065 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L.EctomycorrhizaeStone pineRhizotronAdventitious rootsAcclimationBiotizationPinus pinea L..Escola Superior de Tecnologias e GestãoPinus pinea L. (stone pine) is one of the major plantation species in Iberian Peninsula, being Portugal the largest edible seed producer in the world. The induction and improvement of in vitro rhizogenesis of microshoots of Pinus pinea was developed in our laboratory using a co-culture system with ECM fungi. In the acclimation phase in mixed substrates, or in rhizotrons, anatomical and morphological studies were done to observe the evolution of the root system in microshoots from the co-culture system vs. control plants. Extensive dichotomous and coralloid branching of lateral roots occurred spontaneously in inoculated and control plants as well. Moreover, similar branching occurred in liquid culture of excised seedling roots without the presence of ECM fungi. The striking similarity of these organs with pine ectomycorrhizas prompted their anatomical analysis; however the presence of Hartig net was not confirmed. These results suggested that the development of ECM-like structures might have occurred spontaneously.International Society for Horticultural ScienceDigitUMaCastro, M. R.Ragonezi, C.Klimaszewska, K.Lima, M.de Oliveira, P.Zavattieri, M. A.2022-02-09T09:33:46Z2010-01-01T00:00:00Z2010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/4065eng10.17660/ActaHortic.2010.865.22info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:57:12Zoai:digituma.uma.pt:10400.13/4065Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:53.314164Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L. |
title |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L. |
spellingShingle |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L. Castro, M. R. Ectomycorrhizae Stone pine Rhizotron Adventitious roots Acclimation Biotization Pinus pinea L. . Escola Superior de Tecnologias e Gestão |
title_short |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L. |
title_full |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L. |
title_fullStr |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L. |
title_full_unstemmed |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L. |
title_sort |
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L. |
author |
Castro, M. R. |
author_facet |
Castro, M. R. Ragonezi, C. Klimaszewska, K. Lima, M. de Oliveira, P. Zavattieri, M. A. |
author_role |
author |
author2 |
Ragonezi, C. Klimaszewska, K. Lima, M. de Oliveira, P. Zavattieri, M. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Castro, M. R. Ragonezi, C. Klimaszewska, K. Lima, M. de Oliveira, P. Zavattieri, M. A. |
dc.subject.por.fl_str_mv |
Ectomycorrhizae Stone pine Rhizotron Adventitious roots Acclimation Biotization Pinus pinea L. . Escola Superior de Tecnologias e Gestão |
topic |
Ectomycorrhizae Stone pine Rhizotron Adventitious roots Acclimation Biotization Pinus pinea L. . Escola Superior de Tecnologias e Gestão |
description |
Pinus pinea L. (stone pine) is one of the major plantation species in Iberian Peninsula, being Portugal the largest edible seed producer in the world. The induction and improvement of in vitro rhizogenesis of microshoots of Pinus pinea was developed in our laboratory using a co-culture system with ECM fungi. In the acclimation phase in mixed substrates, or in rhizotrons, anatomical and morphological studies were done to observe the evolution of the root system in microshoots from the co-culture system vs. control plants. Extensive dichotomous and coralloid branching of lateral roots occurred spontaneously in inoculated and control plants as well. Moreover, similar branching occurred in liquid culture of excised seedling roots without the presence of ECM fungi. The striking similarity of these organs with pine ectomycorrhizas prompted their anatomical analysis; however the presence of Hartig net was not confirmed. These results suggested that the development of ECM-like structures might have occurred spontaneously. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01T00:00:00Z 2010-01-01T00:00:00Z 2022-02-09T09:33:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/4065 |
url |
http://hdl.handle.net/10400.13/4065 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.17660/ActaHortic.2010.865.22 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Society for Horticultural Science |
publisher.none.fl_str_mv |
International Society for Horticultural Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799129946610728960 |