Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation

Detalhes bibliográficos
Autor(a) principal: Gonçalves, A. I.
Data de Publicação: 2018
Outros Autores: Rotherham, M., Markides, H., Rodrigues, M. T., Reis, R. L., Gomes, M. E., Haj, A. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/56280
Resumo: Stem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magnetic nanoparticles (MNPs) through remote magnetic actuation resulting in cell differentiation. In this work, we target the Activin receptor type IIA (ActRIIA) in human adipose stem cells (hASCs), using anti-ActRIIA functionalized MNPs, externally activated through a oscillating magnetic bioreactor. Upon activation, the phosphorylation of Smad2/3 is induced allowing translocation of the complex to the nucleus, regulating tenogenic transcriptional responses. Our study demonstrates the potential remote activation of MNPs tagged hASCs to trigger the Activin receptor leading to tenogenic differentiation. These results may provide insights toward tendon regeneration therapies.
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spelling Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulationActivin A receptorHuman adipose derived stem cellsMagnetic nanoparticlesTendon Tissue EngineeringTGF-β/Smad2/3 signaling pathwayActivin A receptorTGF-beta/Smad2/3 signaling pathwayScience & TechnologyStem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magnetic nanoparticles (MNPs) through remote magnetic actuation resulting in cell differentiation. In this work, we target the Activin receptor type IIA (ActRIIA) in human adipose stem cells (hASCs), using anti-ActRIIA functionalized MNPs, externally activated through a oscillating magnetic bioreactor. Upon activation, the phosphorylation of Smad2/3 is induced allowing translocation of the complex to the nucleus, regulating tenogenic transcriptional responses. Our study demonstrates the potential remote activation of MNPs tagged hASCs to trigger the Activin receptor leading to tenogenic differentiation. These results may provide insights toward tendon regeneration therapies.The authors acknowledge the financial support from the European Union Framework Programme for Research and Innovation HORIZON 2020, under the TEAMING Grant agreement No 739572 - The Discoveries CTR. This article is also a result of the project (Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechnologies, marine-derived biomaterials and stem cells), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoGonçalves, A. I.Rotherham, M.Markides, H.Rodrigues, M. T.Reis, R. L.Gomes, M. E.Haj, A. J.2018-062018-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/56280engGonçalves A. I., Rotherham M., Markides H., Rodrigues M. T., Reis R. L., Gomes M. E., Haj A. E. Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation, Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 14, Issue 4, pp. 1149-1159, doi:10.1016/j.nano.2018.02.008, 20181549-963410.1016/j.nano.2018.02.00829471171http://www.nanomedjournal.com/article/S1549-9634(18)30041-8/fulltextinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:22:50Zoai:repositorium.sdum.uminho.pt:1822/56280Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:16:26.335488Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
title Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
spellingShingle Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
Gonçalves, A. I.
Activin A receptor
Human adipose derived stem cells
Magnetic nanoparticles
Tendon Tissue Engineering
TGF-β/Smad2/3 signaling pathway
Activin A receptor
TGF-beta/Smad2/3 signaling pathway
Science & Technology
title_short Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
title_full Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
title_fullStr Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
title_full_unstemmed Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
title_sort Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation
author Gonçalves, A. I.
author_facet Gonçalves, A. I.
Rotherham, M.
Markides, H.
Rodrigues, M. T.
Reis, R. L.
Gomes, M. E.
Haj, A. J.
author_role author
author2 Rotherham, M.
Markides, H.
Rodrigues, M. T.
Reis, R. L.
Gomes, M. E.
Haj, A. J.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gonçalves, A. I.
Rotherham, M.
Markides, H.
Rodrigues, M. T.
Reis, R. L.
Gomes, M. E.
Haj, A. J.
dc.subject.por.fl_str_mv Activin A receptor
Human adipose derived stem cells
Magnetic nanoparticles
Tendon Tissue Engineering
TGF-β/Smad2/3 signaling pathway
Activin A receptor
TGF-beta/Smad2/3 signaling pathway
Science & Technology
topic Activin A receptor
Human adipose derived stem cells
Magnetic nanoparticles
Tendon Tissue Engineering
TGF-β/Smad2/3 signaling pathway
Activin A receptor
TGF-beta/Smad2/3 signaling pathway
Science & Technology
description Stem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magnetic nanoparticles (MNPs) through remote magnetic actuation resulting in cell differentiation. In this work, we target the Activin receptor type IIA (ActRIIA) in human adipose stem cells (hASCs), using anti-ActRIIA functionalized MNPs, externally activated through a oscillating magnetic bioreactor. Upon activation, the phosphorylation of Smad2/3 is induced allowing translocation of the complex to the nucleus, regulating tenogenic transcriptional responses. Our study demonstrates the potential remote activation of MNPs tagged hASCs to trigger the Activin receptor leading to tenogenic differentiation. These results may provide insights toward tendon regeneration therapies.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
2018-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/56280
url http://hdl.handle.net/1822/56280
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gonçalves A. I., Rotherham M., Markides H., Rodrigues M. T., Reis R. L., Gomes M. E., Haj A. E. Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation, Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 14, Issue 4, pp. 1149-1159, doi:10.1016/j.nano.2018.02.008, 2018
1549-9634
10.1016/j.nano.2018.02.008
29471171
http://www.nanomedjournal.com/article/S1549-9634(18)30041-8/fulltext
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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