Microbiological contamination assessment in higher education institutes

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2021
Outros Autores: Pimenta, Raquel, Dias, Marta, Gomes, Bianca, Brito, Miguel, Aranha Caetano, Liliana, Carolino, Elisabete, Gomes, Anita Quintal
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/125825
Resumo: Funding Information: Funding: This work was supported by Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “IPL MOMENTO ZERO” (ESTeSL/IPL/2020). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020. Funding Information: This work was supported by Instituto Polit?cnico de Lisboa, Lisbon, Portugal for funding the Project ?IPL MOMENTO ZERO? (ESTeSL/IPL/2020). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Microbiological contamination assessment in higher education institutesActive and passive samplingAspergillus section FumigatiAzole resistance screeningBacteriaFungiEnvironmental Science (miscellaneous)Funding Information: Funding: This work was supported by Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “IPL MOMENTO ZERO” (ESTeSL/IPL/2020). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020. Funding Information: This work was supported by Instituto Polit?cnico de Lisboa, Lisbon, Portugal for funding the Project ?IPL MOMENTO ZERO? (ESTeSL/IPL/2020). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.The higher education sector represents a unique environment and it acts as a work environment, a learning environment for students, and frequently, also a home environment. The aim of this study was to determine the microbial contamination (SARS-CoV-2, fungi, and bacteria) in Higher Education Facilities (HEI) by using active and passive sampling methods and combining culture-based methods with molecular tools targeting Aspergillus section Fumigati. In addition, the resistance to azole profile was also assessed. Surface samples showed a range of total bacterial contamination between 1 × 103 to 3.1 × 106 CFU·m−2, while Gram-negative bacteria ranged from 0 to 1.9 × 104 CFU·m−2. Fungal contamination ranged from 2 × 103 to 1.8 × 105 CFU·m−2 on MEA, and from 5 × 103 to 1.7 × 105 CFU·m−2 on DG18. The most prevalent species found on both media was Cladosporium sp. (47.36% MEA; 32.33% DG18). Aspergillus genera was observed on MEA (3.21%) and DG18 (14.66%), but not in the supplemented media used for the azole screening. Aspergillus section Fumigati was detected in 2 air samples (2.22%, 2 out of 90 samples) by qPCR. When testing for SARS-CoV-2 all results were negative. The present study showed that although cleaning and disinfection procedures are done regularly due to the COVID-19 pandemic, being effective in eliminating SARS-CoV-2, surfaces were often contaminated with microorganisms other than SARS-CoV-2. This can be a result of increasing resistance to biocides, and to the wide range of environmental factors that can contribute to the dissemination of microbial contamination indoors.Comprehensive Health Research Centre (CHRC) - Pólo ENSPCentro de Investigação em Saúde Pública (CISP/PHRC)Escola Nacional de Saúde Pública (ENSP)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNViegas, CarlaPimenta, RaquelDias, MartaGomes, BiancaBrito, MiguelAranha Caetano, LilianaCarolino, ElisabeteGomes, Anita Quintal2021-10-09T04:58:37Z2021-082021-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/125825eng2073-4433PURE: 34159379https://doi.org/10.3390/atmos12081079info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:06:38Zoai:run.unl.pt:10362/125825Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:47.940817Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microbiological contamination assessment in higher education institutes
title Microbiological contamination assessment in higher education institutes
spellingShingle Microbiological contamination assessment in higher education institutes
Viegas, Carla
Active and passive sampling
Aspergillus section Fumigati
Azole resistance screening
Bacteria
Fungi
Environmental Science (miscellaneous)
title_short Microbiological contamination assessment in higher education institutes
title_full Microbiological contamination assessment in higher education institutes
title_fullStr Microbiological contamination assessment in higher education institutes
title_full_unstemmed Microbiological contamination assessment in higher education institutes
title_sort Microbiological contamination assessment in higher education institutes
author Viegas, Carla
author_facet Viegas, Carla
Pimenta, Raquel
Dias, Marta
Gomes, Bianca
Brito, Miguel
Aranha Caetano, Liliana
Carolino, Elisabete
Gomes, Anita Quintal
author_role author
author2 Pimenta, Raquel
Dias, Marta
Gomes, Bianca
Brito, Miguel
Aranha Caetano, Liliana
Carolino, Elisabete
Gomes, Anita Quintal
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comprehensive Health Research Centre (CHRC) - Pólo ENSP
Centro de Investigação em Saúde Pública (CISP/PHRC)
Escola Nacional de Saúde Pública (ENSP)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Viegas, Carla
Pimenta, Raquel
Dias, Marta
Gomes, Bianca
Brito, Miguel
Aranha Caetano, Liliana
Carolino, Elisabete
Gomes, Anita Quintal
dc.subject.por.fl_str_mv Active and passive sampling
Aspergillus section Fumigati
Azole resistance screening
Bacteria
Fungi
Environmental Science (miscellaneous)
topic Active and passive sampling
Aspergillus section Fumigati
Azole resistance screening
Bacteria
Fungi
Environmental Science (miscellaneous)
description Funding Information: Funding: This work was supported by Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “IPL MOMENTO ZERO” (ESTeSL/IPL/2020). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020. Funding Information: This work was supported by Instituto Polit?cnico de Lisboa, Lisbon, Portugal for funding the Project ?IPL MOMENTO ZERO? (ESTeSL/IPL/2020). H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-09T04:58:37Z
2021-08
2021-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/125825
url http://hdl.handle.net/10362/125825
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2073-4433
PURE: 34159379
https://doi.org/10.3390/atmos12081079
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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