Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses

Detalhes bibliográficos
Autor(a) principal: Conde, Artur
Data de Publicação: 2011
Outros Autores: Silva, Paulo, Agasse, A., Conde, Carlos, Gerós, H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/15873
Resumo: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version is available online at http://pcp.oxfordjournals.org/cgi/content/abstract/pcr121? ijkey=6orgUM5fkIjedYn&keytype=ref
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spelling Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stressesEnvironmental stressMannitolMannitol dehydrogenaseMembrane transportOsmoprotectantScience & TechnologyThis is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version is available online at http://pcp.oxfordjournals.org/cgi/content/abstract/pcr121? ijkey=6orgUM5fkIjedYn&keytype=refThe intracellular accumulation of organic compatible solutes functioning as osmoprotectants, such as polyols, is an important response mechanism of several plants to drought and salinity. In Olea europaea a mannitol transport system (OeMaT1) was previously characterised as a key player in plant response to salinity. In the present study, heterotrophic sink models, such as olive cell suspensions and fruit tissues, and source leaves were used for analytical, biochemical and molecular studies. The kinetic parameters of mannitol dehydrogenase (MTD) determined in mannitol-growing cells, at 25 °C and pH 9.0, were as follows: Km, 54.5 mM mannitol and Vmax, 0.47 μmol h-1 mg-1 protein. The corresponding cDNA was cloned and named OeMTD1. OeMTD1 expression was correlated with MTD activity, OeMaT1 expression and carrier-mediated mannitol transport, in mannitol- and sucrose-growing cells. Furthermore, sucrosegrowing cells displayed only residual OeMTD activity, even though high levels of OeMTD1 transcription were observed. There is evidence OeMTD is regulated at both transcriptional and post-transcriptional levels. MTD activity and OeMTD1 expression were repressed after Na+, K+ and PEG treatments, both in mannitol- and sucrose-growing cells. In contrast, salt and drought significantly increased mannitol transport activity and OeMaT1 expression. Altogether, these studies support that olive tree copes with salinity and drought by coordinating mannitol transport with intracellular metabolism.This work was supported by the Portuguese Foundation for Science and Technology (FCT) (research project ref. PTDC/AGR-ALI/100636/2008; to A. Conde, grant ref. SFRH/BD/47699/2008; to C. Conde, grant ref. SFRH/BPD/34998/2007; to P. Silvagrant ref. SFRH/BD/13460/2003).Oxford University PressUniversidade do MinhoConde, ArturSilva, PauloAgasse, A.Conde, CarlosGerós, H.2011-102011-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/15873eng0032-078110.1093/pcp/pcr12121893515The original publication is available at http://pcp.oxfordjournals.org/content/early/2011/09/04/pcp.pcr121.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:09Zoai:repositorium.sdum.uminho.pt:1822/15873Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:02:52.327331Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses
title Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses
spellingShingle Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses
Conde, Artur
Environmental stress
Mannitol
Mannitol dehydrogenase
Membrane transport
Osmoprotectant
Science & Technology
title_short Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses
title_full Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses
title_fullStr Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses
title_full_unstemmed Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses
title_sort Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses
author Conde, Artur
author_facet Conde, Artur
Silva, Paulo
Agasse, A.
Conde, Carlos
Gerós, H.
author_role author
author2 Silva, Paulo
Agasse, A.
Conde, Carlos
Gerós, H.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Conde, Artur
Silva, Paulo
Agasse, A.
Conde, Carlos
Gerós, H.
dc.subject.por.fl_str_mv Environmental stress
Mannitol
Mannitol dehydrogenase
Membrane transport
Osmoprotectant
Science & Technology
topic Environmental stress
Mannitol
Mannitol dehydrogenase
Membrane transport
Osmoprotectant
Science & Technology
description This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version is available online at http://pcp.oxfordjournals.org/cgi/content/abstract/pcr121? ijkey=6orgUM5fkIjedYn&keytype=ref
publishDate 2011
dc.date.none.fl_str_mv 2011-10
2011-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/15873
url http://hdl.handle.net/1822/15873
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0032-0781
10.1093/pcp/pcr121
21893515
The original publication is available at http://pcp.oxfordjournals.org/content/early/2011/09/04/pcp.pcr121.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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