Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/19985 |
Resumo: | Bovine mastitis (BM) presents a high incidence, being Staphylococcus aureus one of the major causative agents. Antibiotics comprise the most common therapeutic approach, but due to their indiscriminate use, high rates of increasingly resistant bacterial species have been markedly pointed out. Particularly, S. aureus possesses a pronounced ability to form biofilms, and therefore, are of pivotal interest due to its alarming pathogenicity. The present study investigates the antibacterial properties of Eucalyptus globulus methanol: water extracts, alone and in combination with Juglans regia, against S. aureus isolates from BM. All isolates and reference strain proved to be good biofilm producers after 24 h of bacterial growth. Individually, the studied plant extracts (PE) lead to a considerable biofilm cells reduction, but their combination revealed to be the most effective strategy. When tested in combination, both extracts led to a 3 and 5 log reduction for S. aureus ATCC 25923 and S. aureus 1, respectively. Based on these findings, both PE seem to be promissory antimicrobial agents for upcoming use on dairy industry contaminations, BM and even S. aureus-triggered food poisoning. Further studies are needed to understand which of the compounds present in the extracts are responsible for the observed effects, including their corresponding modes of action. |
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Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitisMicrobiologyBovine mastitis (BM) presents a high incidence, being Staphylococcus aureus one of the major causative agents. Antibiotics comprise the most common therapeutic approach, but due to their indiscriminate use, high rates of increasingly resistant bacterial species have been markedly pointed out. Particularly, S. aureus possesses a pronounced ability to form biofilms, and therefore, are of pivotal interest due to its alarming pathogenicity. The present study investigates the antibacterial properties of Eucalyptus globulus methanol: water extracts, alone and in combination with Juglans regia, against S. aureus isolates from BM. All isolates and reference strain proved to be good biofilm producers after 24 h of bacterial growth. Individually, the studied plant extracts (PE) lead to a considerable biofilm cells reduction, but their combination revealed to be the most effective strategy. When tested in combination, both extracts led to a 3 and 5 log reduction for S. aureus ATCC 25923 and S. aureus 1, respectively. Based on these findings, both PE seem to be promissory antimicrobial agents for upcoming use on dairy industry contaminations, BM and even S. aureus-triggered food poisoning. Further studies are needed to understand which of the compounds present in the extracts are responsible for the observed effects, including their corresponding modes of action.This work was supported by the Portuguese Foundation for Science and Technology (SFRH/BPD/84488/2012) and by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. N. Martins thank the Portuguese Foundation for Science and Technology (FCT-Portugal) for the Strategic project ref. UID/BIM/04293/2013 and "NORTE2020 - Northern Regional Operational Program" (NORTE-01-0145-FEDER-000012).Biblioteca Digital do IPBGomes, FernandaMartins, NatáliaFerreira, Isabel C.F.R.Henriques, Mariana2018-01-19T10:00:00Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/19985engGomes, Fernanda; Martins, Natália; Ferreira, Isabel C.F.R.; Henriques, Mariana (2019). Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis. Heliyon. ISSN 2405-8440. 5, p.2405-844010.1016/j.heliyon.2019.e01728info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-16T12:21:22ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis |
title |
Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis |
spellingShingle |
Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis Gomes, Fernanda Microbiology |
title_short |
Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis |
title_full |
Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis |
title_fullStr |
Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis |
title_full_unstemmed |
Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis |
title_sort |
Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis |
author |
Gomes, Fernanda |
author_facet |
Gomes, Fernanda Martins, Natália Ferreira, Isabel C.F.R. Henriques, Mariana |
author_role |
author |
author2 |
Martins, Natália Ferreira, Isabel C.F.R. Henriques, Mariana |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Gomes, Fernanda Martins, Natália Ferreira, Isabel C.F.R. Henriques, Mariana |
dc.subject.por.fl_str_mv |
Microbiology |
topic |
Microbiology |
description |
Bovine mastitis (BM) presents a high incidence, being Staphylococcus aureus one of the major causative agents. Antibiotics comprise the most common therapeutic approach, but due to their indiscriminate use, high rates of increasingly resistant bacterial species have been markedly pointed out. Particularly, S. aureus possesses a pronounced ability to form biofilms, and therefore, are of pivotal interest due to its alarming pathogenicity. The present study investigates the antibacterial properties of Eucalyptus globulus methanol: water extracts, alone and in combination with Juglans regia, against S. aureus isolates from BM. All isolates and reference strain proved to be good biofilm producers after 24 h of bacterial growth. Individually, the studied plant extracts (PE) lead to a considerable biofilm cells reduction, but their combination revealed to be the most effective strategy. When tested in combination, both extracts led to a 3 and 5 log reduction for S. aureus ATCC 25923 and S. aureus 1, respectively. Based on these findings, both PE seem to be promissory antimicrobial agents for upcoming use on dairy industry contaminations, BM and even S. aureus-triggered food poisoning. Further studies are needed to understand which of the compounds present in the extracts are responsible for the observed effects, including their corresponding modes of action. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/19985 |
url |
http://hdl.handle.net/10198/19985 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gomes, Fernanda; Martins, Natália; Ferreira, Isabel C.F.R.; Henriques, Mariana (2019). Anti-biofilm activity of hydromethanolic plant extracts against Staphylococcus aureus isolates from bovine mastitis. Heliyon. ISSN 2405-8440. 5, p. 2405-8440 10.1016/j.heliyon.2019.e01728 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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repository.mail.fl_str_mv |
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1777301899942297600 |