A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent

Detalhes bibliográficos
Autor(a) principal: Branco, Patrícia
Data de Publicação: 2018
Outros Autores: Candeias, António, Caldeira, Ana Teresa, González-Pérez, Marina
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/23253
Resumo: Thus, the aim of this study was to design in silico a novel RNA-FISH probe to Rhodotorula sp., that facilitate a precise identification of this yeast, and to evaluate its specificity and experimental performance. The probe designed was a novel 26S RNA probe whose experimental evaluation was done by constructing the fluorescence-signal-response/formamide concentration curve for the target and a non-target yeast (Cryptococcus adeliensis). In silico and experimental results revealed that the Rhodotorula sp. probe is genus-specific. Hence, this study will contribute to an accurate identification of microorganisms involved in the biodeterioration process, advantageous for the selection of proper remediation strategies.
id RCAP_9dff683b305224e13b861b54d5bc5987
oai_identifier_str oai:dspace.uevora.pt:10174/23253
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agentThus, the aim of this study was to design in silico a novel RNA-FISH probe to Rhodotorula sp., that facilitate a precise identification of this yeast, and to evaluate its specificity and experimental performance. The probe designed was a novel 26S RNA probe whose experimental evaluation was done by constructing the fluorescence-signal-response/formamide concentration curve for the target and a non-target yeast (Cryptococcus adeliensis). In silico and experimental results revealed that the Rhodotorula sp. probe is genus-specific. Hence, this study will contribute to an accurate identification of microorganisms involved in the biodeterioration process, advantageous for the selection of proper remediation strategies.This work was co-financed by PTDC/BBB-IMG/0046/2014-FCT and ALT20-03-0246-FEDER-000004-ALENTEJO 2020 project. Marina González-Pérez acknowledges FCT for the economic support through the post-doctoral grant SFRH/BPD/100754/2014.Universidade de Évora2018-06-13T11:47:20Z2018-06-132018-05-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/23253http://hdl.handle.net/10174/23253engBranco, P.; Candeias; A.; Caldeira, A. T.; González-Pérez, M. A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent (2018). In Web of Knowledge: A look into the Past, embracing the Future, Universidade de Évora, Portugal. pp.74 .http://www.wok.uevora.pt/naonaosimpbranco@uevora.ptcandeias@uevora.ptatc@uevora.ptmarinagp@uevora.ptBranco, PatríciaCandeias, AntónioCaldeira, Ana TeresaGonzález-Pérez, Marinainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:15:09Zoai:dspace.uevora.pt:10174/23253Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:14:04.506138Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent
title A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent
spellingShingle A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent
Branco, Patrícia
title_short A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent
title_full A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent
title_fullStr A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent
title_full_unstemmed A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent
title_sort A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent
author Branco, Patrícia
author_facet Branco, Patrícia
Candeias, António
Caldeira, Ana Teresa
González-Pérez, Marina
author_role author
author2 Candeias, António
Caldeira, Ana Teresa
González-Pérez, Marina
author2_role author
author
author
dc.contributor.author.fl_str_mv Branco, Patrícia
Candeias, António
Caldeira, Ana Teresa
González-Pérez, Marina
description Thus, the aim of this study was to design in silico a novel RNA-FISH probe to Rhodotorula sp., that facilitate a precise identification of this yeast, and to evaluate its specificity and experimental performance. The probe designed was a novel 26S RNA probe whose experimental evaluation was done by constructing the fluorescence-signal-response/formamide concentration curve for the target and a non-target yeast (Cryptococcus adeliensis). In silico and experimental results revealed that the Rhodotorula sp. probe is genus-specific. Hence, this study will contribute to an accurate identification of microorganisms involved in the biodeterioration process, advantageous for the selection of proper remediation strategies.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-13T11:47:20Z
2018-06-13
2018-05-17T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/23253
http://hdl.handle.net/10174/23253
url http://hdl.handle.net/10174/23253
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Branco, P.; Candeias; A.; Caldeira, A. T.; González-Pérez, M. A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent (2018). In Web of Knowledge: A look into the Past, embracing the Future, Universidade de Évora, Portugal. pp.74 .
http://www.wok.uevora.pt/
nao
nao
sim
pbranco@uevora.pt
candeias@uevora.pt
atc@uevora.pt
marinagp@uevora.pt
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de Évora
publisher.none.fl_str_mv Universidade de Évora
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136622889926656

Registros relacionados