Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.19/4106 |
Resumo: | Coastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 10(1) to 8.6 × 10(4) RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans. |
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Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), SpainDetectionHepatitis E virusShellfishCoastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 10(1) to 8.6 × 10(4) RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans.ElsevierRepositório Científico do Instituto Politécnico de ViseuMesquita, João R.Oliveira, DanielleRivadulla, EnriqueSilva, Joana AbreuVarela, Miguel F.Romalde, Jesús L.Nascimento, Maria S. J.2017-01-26T13:20:44Z2016-092016-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.19/4106engMesquita, J. R., Oliveira, D., Rivadulla, E., Silva, J. A., Varela, M. F., Romalde, J. L., & Nascimento, M. S. J. (2016). Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain. Food Microbiology, 58, 13-15. doi:10.1016/j.fm.2016.03.009.10.1016/j.fm.2016.03.009metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-16T15:27:01ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain |
title |
Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain |
spellingShingle |
Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain Mesquita, João R. Detection Hepatitis E virus Shellfish |
title_short |
Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain |
title_full |
Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain |
title_fullStr |
Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain |
title_full_unstemmed |
Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain |
title_sort |
Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain |
author |
Mesquita, João R. |
author_facet |
Mesquita, João R. Oliveira, Danielle Rivadulla, Enrique Silva, Joana Abreu Varela, Miguel F. Romalde, Jesús L. Nascimento, Maria S. J. |
author_role |
author |
author2 |
Oliveira, Danielle Rivadulla, Enrique Silva, Joana Abreu Varela, Miguel F. Romalde, Jesús L. Nascimento, Maria S. J. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico de Viseu |
dc.contributor.author.fl_str_mv |
Mesquita, João R. Oliveira, Danielle Rivadulla, Enrique Silva, Joana Abreu Varela, Miguel F. Romalde, Jesús L. Nascimento, Maria S. J. |
dc.subject.por.fl_str_mv |
Detection Hepatitis E virus Shellfish |
topic |
Detection Hepatitis E virus Shellfish |
description |
Coastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 10(1) to 8.6 × 10(4) RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09 2016-09-01T00:00:00Z 2017-01-26T13:20:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.19/4106 |
url |
http://hdl.handle.net/10400.19/4106 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mesquita, J. R., Oliveira, D., Rivadulla, E., Silva, J. A., Varela, M. F., Romalde, J. L., & Nascimento, M. S. J. (2016). Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain. Food Microbiology, 58, 13-15. doi:10.1016/j.fm.2016.03.009. 10.1016/j.fm.2016.03.009 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777301965163724800 |