Occluding junctions as novel regulators of tissue mechanics during wound repair

Detalhes bibliográficos
Autor(a) principal: Carvalho, Lara
Data de Publicação: 2018
Outros Autores: Patricio, Pedro, Ponte, Susana, Heisenberg, Carl Philipp, Almeida, Luis, Nunes, André S., Araújo, Nuno A.M., Jacinto, Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/145094
Resumo: This research was supported by Fundação para a Ciência e a Tecnologia (SFRH/BPD/84569/2012 to L. Carvalho, PD/ BD/106058/2015 to S. Ponte, SFRH/BD/119240/2016 to A.S. Nunes, UID/FIS/00618/2013, PTDC/FIS-MAC/28146/2017, PTDC/ BIA-BID/29709/2017 and CONGENTO), the European Research Council (2007-StG-208631 and ERC-2015-PoC-713735-EMODI), a European Commission Marie Curie Intra-European Fellowship (PIEF-GA-2009-255573), the Partenariat Hubert Curien Pessoa program (30920XM), the European Science Foundation (Quantitative Models of Cellular and Developmental Biology Research Networking Program, grant 6839), and the iNOVA4Health Research Unit (UID/Multi/04462/2013), cofunded by Fundação para a Ciência e Tecnologia, through national funds and by FED ER under the PT2020 Partnership Agreement.
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spelling Occluding junctions as novel regulators of tissue mechanics during wound repairCell BiologyThis research was supported by Fundação para a Ciência e a Tecnologia (SFRH/BPD/84569/2012 to L. Carvalho, PD/ BD/106058/2015 to S. Ponte, SFRH/BD/119240/2016 to A.S. Nunes, UID/FIS/00618/2013, PTDC/FIS-MAC/28146/2017, PTDC/ BIA-BID/29709/2017 and CONGENTO), the European Research Council (2007-StG-208631 and ERC-2015-PoC-713735-EMODI), a European Commission Marie Curie Intra-European Fellowship (PIEF-GA-2009-255573), the Partenariat Hubert Curien Pessoa program (30920XM), the European Science Foundation (Quantitative Models of Cellular and Developmental Biology Research Networking Program, grant 6839), and the iNOVA4Health Research Unit (UID/Multi/04462/2013), cofunded by Fundação para a Ciência e Tecnologia, through national funds and by FED ER under the PT2020 Partnership Agreement.In epithelial tissues, cells tightly connect to each other through cell–cell junctions, but they also present the remarkable capacity of reorganizing themselves without compromising tissue integrity. Upon injury, simple epithelia efficiently resolve small lesions through the action of actin cytoskeleton contractile structures at the wound edge and cellular rearrangements. However, the underlying mechanisms and how they cooperate are still poorly understood. In this study, we combine live imaging and theoretical modeling to reveal a novel and indispensable role for occluding junctions (OJs) in this process. We demonstrate that OJ loss of function leads to defects in wound-closure dynamics: instead of contracting, wounds dramatically increase their area. OJ mutants exhibit phenotypes in cell shape, cellular rearrangements, and mechanical properties as well as in actin cytoskeleton dynamics at the wound edge. We propose that OJs are essential for wound closure by impacting on epithelial mechanics at the tissue level, which in turn is crucial for correct regulation of the cellular events occurring at the wound edge.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNCarvalho, LaraPatricio, PedroPonte, SusanaHeisenberg, Carl PhilippAlmeida, LuisNunes, André S.Araújo, Nuno A.M.Jacinto, Antonio2022-10-28T22:05:24Z2018-12-012018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/145094eng0021-9525PURE: 10673705https://doi.org/10.1083/jcb.201804048info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:25:14Zoai:run.unl.pt:10362/145094Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:54.706644Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Occluding junctions as novel regulators of tissue mechanics during wound repair
title Occluding junctions as novel regulators of tissue mechanics during wound repair
spellingShingle Occluding junctions as novel regulators of tissue mechanics during wound repair
Carvalho, Lara
Cell Biology
title_short Occluding junctions as novel regulators of tissue mechanics during wound repair
title_full Occluding junctions as novel regulators of tissue mechanics during wound repair
title_fullStr Occluding junctions as novel regulators of tissue mechanics during wound repair
title_full_unstemmed Occluding junctions as novel regulators of tissue mechanics during wound repair
title_sort Occluding junctions as novel regulators of tissue mechanics during wound repair
author Carvalho, Lara
author_facet Carvalho, Lara
Patricio, Pedro
Ponte, Susana
Heisenberg, Carl Philipp
Almeida, Luis
Nunes, André S.
Araújo, Nuno A.M.
Jacinto, Antonio
author_role author
author2 Patricio, Pedro
Ponte, Susana
Heisenberg, Carl Philipp
Almeida, Luis
Nunes, André S.
Araújo, Nuno A.M.
Jacinto, Antonio
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Carvalho, Lara
Patricio, Pedro
Ponte, Susana
Heisenberg, Carl Philipp
Almeida, Luis
Nunes, André S.
Araújo, Nuno A.M.
Jacinto, Antonio
dc.subject.por.fl_str_mv Cell Biology
topic Cell Biology
description This research was supported by Fundação para a Ciência e a Tecnologia (SFRH/BPD/84569/2012 to L. Carvalho, PD/ BD/106058/2015 to S. Ponte, SFRH/BD/119240/2016 to A.S. Nunes, UID/FIS/00618/2013, PTDC/FIS-MAC/28146/2017, PTDC/ BIA-BID/29709/2017 and CONGENTO), the European Research Council (2007-StG-208631 and ERC-2015-PoC-713735-EMODI), a European Commission Marie Curie Intra-European Fellowship (PIEF-GA-2009-255573), the Partenariat Hubert Curien Pessoa program (30920XM), the European Science Foundation (Quantitative Models of Cellular and Developmental Biology Research Networking Program, grant 6839), and the iNOVA4Health Research Unit (UID/Multi/04462/2013), cofunded by Fundação para a Ciência e Tecnologia, through national funds and by FED ER under the PT2020 Partnership Agreement.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
2018-12-01T00:00:00Z
2022-10-28T22:05:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/145094
url http://hdl.handle.net/10362/145094
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9525
PURE: 10673705
https://doi.org/10.1083/jcb.201804048
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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