MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation

Detalhes bibliográficos
Autor(a) principal: Graça, Clara Susana Marques
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/6788
Resumo: Mestrado em Biologia Funcional - Instituto Superior de Agronomia
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spelling MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validationEucalyptus globuluswood formationmiRNAstarget genesRLM 5'-RACEMestrado em Biologia Funcional - Instituto Superior de AgronomiaPortugal is one of the largest producers of pulp and paper derived from Eucalyptus globulus, which makes this a valuable species for the country. Wood is a complex and variable material, and its posttranscriptional regulation knowledge is only beginning. MicroRNAs (miRNA) are small size (21-24nt), endogenous non-coding RNAs, involved in post-transcriptional regulation. MiRBase v20 database encloses thousands of entries, however none from Eucalyptus. In this study we aim to validate E. globulus miRNAs candidates; to predict in silico and validate experimentally the miRNAs targets; and analyze the gene expression of validated targets. Four miRCa-02, miRCa-04, miRCa-08 and miRCa-09 candidates were validated by Northern blot and there in silico prediction revealed 42 target genes. Fourteen predicted target genes were tested through the RLM 5’-RACE methodology, but only three predicted targets were validated (Eucgr.E01509, Eucgr.C01382 and Eucgr.J02113 predicted target genes for miR171, miRCa-04 and miRCa-08, respectively). Expression of these three target genes analyzed by RT-qPCR suggests that the distinct expression levels found may be related with to wood formation in Eucalyptus globulus. For the first time, four Eucalytus miRNAs and their target genes were disclosed and validated by bioinformatic and molecular tools.ISAPaiva, Jorge PintoCecílio, Leonor MoraisRepositório da Universidade de LisboaGraça, Clara Susana Marques2014-05-15T13:04:09Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/6788TID:201845792engGraça, C.S.M. - MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation. Lisboa: ISA, 2014, 93 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:37:36Zoai:www.repository.utl.pt:10400.5/6788Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:54:06.113728Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation
title MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation
spellingShingle MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation
Graça, Clara Susana Marques
Eucalyptus globulus
wood formation
miRNAs
target genes
RLM 5'-RACE
title_short MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation
title_full MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation
title_fullStr MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation
title_full_unstemmed MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation
title_sort MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation
author Graça, Clara Susana Marques
author_facet Graça, Clara Susana Marques
author_role author
dc.contributor.none.fl_str_mv Paiva, Jorge Pinto
Cecílio, Leonor Morais
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Graça, Clara Susana Marques
dc.subject.por.fl_str_mv Eucalyptus globulus
wood formation
miRNAs
target genes
RLM 5'-RACE
topic Eucalyptus globulus
wood formation
miRNAs
target genes
RLM 5'-RACE
description Mestrado em Biologia Funcional - Instituto Superior de Agronomia
publishDate 2014
dc.date.none.fl_str_mv 2014-05-15T13:04:09Z
2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/6788
TID:201845792
url http://hdl.handle.net/10400.5/6788
identifier_str_mv TID:201845792
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Graça, C.S.M. - MicroRNAs and target genes involved in E. globulus xylogenesis: in silico prediction and experimental validation. Lisboa: ISA, 2014, 93 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA
publisher.none.fl_str_mv ISA
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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