Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/40068 |
Resumo: | Galectin-3 (Gal-3) is a carbohydrate-binding protein associated with the development and progress of heart failure. Here, we report the first colorimetric and low-cost approach for detecting and quantifying Gal-3 using gold nanoparticles (AuNPs) bioconjugated with Gal-3 antibody. The interaction of Gal-3 with the resulting nanoprobes led to a linear response of the absorbance ratio A750nm/A526nm to Gal-3 concentration, accompanied by a change in color intensity. The assay showed a linear optical response even in complex samples, such as saliva and fetal bovine serum (FBS), up to a concentration of 200 μg L−1. The limit of detection (LOD) followed the trend LODPBS (10.0 μg L−1) < LODsaliva (22.6 μg L−1) < LODFBS (25.3 μg L−1). The potential applicability of this method to the analysis of human plasma samples was also demonstrated. Compared to conventional detection techniques, this colorimetric assay provides faster results (∼1 h) and is more cost-effective due to the use of simple and unexpensive equipment. This assay represents an exciting solution for the rapid screening of high risk for rapid progression of heart failure in patient samples (Gal-3 > 25.9 μg L−1). |
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Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approachGalectin-3 (Gal-3) is a carbohydrate-binding protein associated with the development and progress of heart failure. Here, we report the first colorimetric and low-cost approach for detecting and quantifying Gal-3 using gold nanoparticles (AuNPs) bioconjugated with Gal-3 antibody. The interaction of Gal-3 with the resulting nanoprobes led to a linear response of the absorbance ratio A750nm/A526nm to Gal-3 concentration, accompanied by a change in color intensity. The assay showed a linear optical response even in complex samples, such as saliva and fetal bovine serum (FBS), up to a concentration of 200 μg L−1. The limit of detection (LOD) followed the trend LODPBS (10.0 μg L−1) < LODsaliva (22.6 μg L−1) < LODFBS (25.3 μg L−1). The potential applicability of this method to the analysis of human plasma samples was also demonstrated. Compared to conventional detection techniques, this colorimetric assay provides faster results (∼1 h) and is more cost-effective due to the use of simple and unexpensive equipment. This assay represents an exciting solution for the rapid screening of high risk for rapid progression of heart failure in patient samples (Gal-3 > 25.9 μg L−1).Royal Society of Chemistry2024-01-11T11:13:49Z2023-03-01T00:00:00Z2023-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/40068eng1759-966010.1039/D3AY00347GAntónio, MariaLima, TâniaFerreira, RitaFardilha, MargaridaBastos, José MesquitaVitorino, RuiDaniel-da-Silva, Ana L.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:18:14Zoai:ria.ua.pt:10773/40068Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:10:04.868020Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach |
title |
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach |
spellingShingle |
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach António, Maria |
title_short |
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach |
title_full |
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach |
title_fullStr |
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach |
title_full_unstemmed |
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach |
title_sort |
Gold nanoparticle probes for colorimetric detection of plasma galectin-3: a simple and rapid approach |
author |
António, Maria |
author_facet |
António, Maria Lima, Tânia Ferreira, Rita Fardilha, Margarida Bastos, José Mesquita Vitorino, Rui Daniel-da-Silva, Ana L. |
author_role |
author |
author2 |
Lima, Tânia Ferreira, Rita Fardilha, Margarida Bastos, José Mesquita Vitorino, Rui Daniel-da-Silva, Ana L. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
António, Maria Lima, Tânia Ferreira, Rita Fardilha, Margarida Bastos, José Mesquita Vitorino, Rui Daniel-da-Silva, Ana L. |
description |
Galectin-3 (Gal-3) is a carbohydrate-binding protein associated with the development and progress of heart failure. Here, we report the first colorimetric and low-cost approach for detecting and quantifying Gal-3 using gold nanoparticles (AuNPs) bioconjugated with Gal-3 antibody. The interaction of Gal-3 with the resulting nanoprobes led to a linear response of the absorbance ratio A750nm/A526nm to Gal-3 concentration, accompanied by a change in color intensity. The assay showed a linear optical response even in complex samples, such as saliva and fetal bovine serum (FBS), up to a concentration of 200 μg L−1. The limit of detection (LOD) followed the trend LODPBS (10.0 μg L−1) < LODsaliva (22.6 μg L−1) < LODFBS (25.3 μg L−1). The potential applicability of this method to the analysis of human plasma samples was also demonstrated. Compared to conventional detection techniques, this colorimetric assay provides faster results (∼1 h) and is more cost-effective due to the use of simple and unexpensive equipment. This assay represents an exciting solution for the rapid screening of high risk for rapid progression of heart failure in patient samples (Gal-3 > 25.9 μg L−1). |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-01T00:00:00Z 2023-03 2024-01-11T11:13:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/40068 |
url |
http://hdl.handle.net/10773/40068 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1759-9660 10.1039/D3AY00347G |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137751095836672 |