Interactions among grapevine disease-causing fungi. The role of reactive oxygen species
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/1521 |
Resumo: | Botryosphaeria parva, Eutypa lata and Phomopsis viticola are ascomyceteous fungi responsible for severe canker and dieback in numerous woody plants. In grapevine, these pathogens colonise the wood mainly through pruning wounds, and the diseases gradually develop, leading to partial or total vine death. In the present study, the three fungal species were grown in Czapek Dox modifi ed medium. Under these conditions, fungal colonies are able to distinguish self from non-self. The production of reactive oxygen species (ROS) was analysed by specifi cally staining for superoxide (O2 .-) or peroxide (O2 2-) radicals. The presence of ROS in both isolated cultures and fungal interactions was confi rmed. All fungi produced both radicals, in every interaction. However, the patterns of ROS production depend on the fungus itself and on the fungal species with which it is interacting, being also dependent on the presence of antioxidant compounds in the surrounding medium. It is as though a fungal species hierarchy could be established for every interaction under each set of conditions (i.e. habitat). The results obtained suggest that fungi display more complex behaviours than generally acknowledged. They are able to recognize potential contestants and built up defence reactions, as well as weaken plant defences and structures to induce infection. |
id |
RCAP_a38ef32b2db5965e3bb6ed3bf6117769 |
---|---|
oai_identifier_str |
oai:www.repository.utl.pt:10400.5/1521 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen speciesbotryosphaeria parvaeuthypa lataphomopsis viticolawood diseasesgrapevineBotryosphaeria parva, Eutypa lata and Phomopsis viticola are ascomyceteous fungi responsible for severe canker and dieback in numerous woody plants. In grapevine, these pathogens colonise the wood mainly through pruning wounds, and the diseases gradually develop, leading to partial or total vine death. In the present study, the three fungal species were grown in Czapek Dox modifi ed medium. Under these conditions, fungal colonies are able to distinguish self from non-self. The production of reactive oxygen species (ROS) was analysed by specifi cally staining for superoxide (O2 .-) or peroxide (O2 2-) radicals. The presence of ROS in both isolated cultures and fungal interactions was confi rmed. All fungi produced both radicals, in every interaction. However, the patterns of ROS production depend on the fungus itself and on the fungal species with which it is interacting, being also dependent on the presence of antioxidant compounds in the surrounding medium. It is as though a fungal species hierarchy could be established for every interaction under each set of conditions (i.e. habitat). The results obtained suggest that fungi display more complex behaviours than generally acknowledged. They are able to recognize potential contestants and built up defence reactions, as well as weaken plant defences and structures to induce infection.Mediterranean Phytopathological UnionRepositório da Universidade de LisboaOliveira, HelenaFreitas, ReginaRego, CeciliaFerreira, Ricardo Boavida2009-11-23T16:45:29Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/1521eng"Phytopathologia Mediterranea". ISSN 0031-9465. 48 (2009) 117-1270031-9465info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:32:46Zoai:www.repository.utl.pt:10400.5/1521Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:49:38.257290Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen species |
title |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen species |
spellingShingle |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen species Oliveira, Helena botryosphaeria parva euthypa lata phomopsis viticola wood diseases grapevine |
title_short |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen species |
title_full |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen species |
title_fullStr |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen species |
title_full_unstemmed |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen species |
title_sort |
Interactions among grapevine disease-causing fungi. The role of reactive oxygen species |
author |
Oliveira, Helena |
author_facet |
Oliveira, Helena Freitas, Regina Rego, Cecilia Ferreira, Ricardo Boavida |
author_role |
author |
author2 |
Freitas, Regina Rego, Cecilia Ferreira, Ricardo Boavida |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Oliveira, Helena Freitas, Regina Rego, Cecilia Ferreira, Ricardo Boavida |
dc.subject.por.fl_str_mv |
botryosphaeria parva euthypa lata phomopsis viticola wood diseases grapevine |
topic |
botryosphaeria parva euthypa lata phomopsis viticola wood diseases grapevine |
description |
Botryosphaeria parva, Eutypa lata and Phomopsis viticola are ascomyceteous fungi responsible for severe canker and dieback in numerous woody plants. In grapevine, these pathogens colonise the wood mainly through pruning wounds, and the diseases gradually develop, leading to partial or total vine death. In the present study, the three fungal species were grown in Czapek Dox modifi ed medium. Under these conditions, fungal colonies are able to distinguish self from non-self. The production of reactive oxygen species (ROS) was analysed by specifi cally staining for superoxide (O2 .-) or peroxide (O2 2-) radicals. The presence of ROS in both isolated cultures and fungal interactions was confi rmed. All fungi produced both radicals, in every interaction. However, the patterns of ROS production depend on the fungus itself and on the fungal species with which it is interacting, being also dependent on the presence of antioxidant compounds in the surrounding medium. It is as though a fungal species hierarchy could be established for every interaction under each set of conditions (i.e. habitat). The results obtained suggest that fungi display more complex behaviours than generally acknowledged. They are able to recognize potential contestants and built up defence reactions, as well as weaken plant defences and structures to induce infection. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-23T16:45:29Z 2009 2009-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/1521 |
url |
http://hdl.handle.net/10400.5/1521 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Phytopathologia Mediterranea". ISSN 0031-9465. 48 (2009) 117-127 0031-9465 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mediterranean Phytopathological Union |
publisher.none.fl_str_mv |
Mediterranean Phytopathological Union |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799130971211038720 |