Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/6898 |
Resumo: | Aims: The aims of this study were to characterize strains of Listeria monocytogenes isolated from cold-smoking fish plants to establish possible routes of contamination through the processing chain. Methods and Results: Listeria monocytogenes from fresh fish suppliers, raw materials, factory sites and finished products isolated in Portugal (162 isolates) and England (28 isolates) were characterized by serotyping, phage typing, tetracycline, cadmium and arsenic resistance, and plasmid profiling. On the basis of serotyping and phage typing, the isolates were categorized into eight groups. Although cultures within some of the groups could be further differentiated on the basis of plasmid profiling and cadmium and arsenite typing, consideration of all typing data predominantly clustered together isolates from a single location. L. monocytogenes strains: from fresh salmon suppliers were not found in the processing lines; from fresh salmon from different locations differed; and from the water where salmon trout were farmed differed from those isolated from the fish samples. Significance and Impact of the Study: No clear source or route of contamination in the cold-smoked processing chain could be established; however, these results highlight the complexity in tracking this bacterium through food chains. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fishAims: The aims of this study were to characterize strains of Listeria monocytogenes isolated from cold-smoking fish plants to establish possible routes of contamination through the processing chain. Methods and Results: Listeria monocytogenes from fresh fish suppliers, raw materials, factory sites and finished products isolated in Portugal (162 isolates) and England (28 isolates) were characterized by serotyping, phage typing, tetracycline, cadmium and arsenic resistance, and plasmid profiling. On the basis of serotyping and phage typing, the isolates were categorized into eight groups. Although cultures within some of the groups could be further differentiated on the basis of plasmid profiling and cadmium and arsenite typing, consideration of all typing data predominantly clustered together isolates from a single location. L. monocytogenes strains: from fresh salmon suppliers were not found in the processing lines; from fresh salmon from different locations differed; and from the water where salmon trout were farmed differed from those isolated from the fish samples. Significance and Impact of the Study: No clear source or route of contamination in the cold-smoked processing chain could be established; however, these results highlight the complexity in tracking this bacterium through food chains.Willey BlackwellVeritati - Repositório Institucional da Universidade Católica PortuguesaVaz-Velho, M.Duarte, G.McLauchlin, J.Gibbs, P.2011-10-22T22:38:22Z20012001-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6898engVAZ-VELHO, M...[et al.] - Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish. Journal of Applied Microbiology. ISSN 1365-2672. Vol. 91, n.º 3 (2001), p. 556–56210.1046/j.1365-2672.2001.01425.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:10:39ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish |
title |
Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish |
spellingShingle |
Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish Vaz-Velho, M. |
title_short |
Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish |
title_full |
Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish |
title_fullStr |
Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish |
title_full_unstemmed |
Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish |
title_sort |
Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish |
author |
Vaz-Velho, M. |
author_facet |
Vaz-Velho, M. Duarte, G. McLauchlin, J. Gibbs, P. |
author_role |
author |
author2 |
Duarte, G. McLauchlin, J. Gibbs, P. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Vaz-Velho, M. Duarte, G. McLauchlin, J. Gibbs, P. |
description |
Aims: The aims of this study were to characterize strains of Listeria monocytogenes isolated from cold-smoking fish plants to establish possible routes of contamination through the processing chain. Methods and Results: Listeria monocytogenes from fresh fish suppliers, raw materials, factory sites and finished products isolated in Portugal (162 isolates) and England (28 isolates) were characterized by serotyping, phage typing, tetracycline, cadmium and arsenic resistance, and plasmid profiling. On the basis of serotyping and phage typing, the isolates were categorized into eight groups. Although cultures within some of the groups could be further differentiated on the basis of plasmid profiling and cadmium and arsenite typing, consideration of all typing data predominantly clustered together isolates from a single location. L. monocytogenes strains: from fresh salmon suppliers were not found in the processing lines; from fresh salmon from different locations differed; and from the water where salmon trout were farmed differed from those isolated from the fish samples. Significance and Impact of the Study: No clear source or route of contamination in the cold-smoked processing chain could be established; however, these results highlight the complexity in tracking this bacterium through food chains. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001 2001-01-01T00:00:00Z 2011-10-22T22:38:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/6898 |
url |
http://hdl.handle.net/10400.14/6898 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
VAZ-VELHO, M...[et al.] - Characterization of Listeria monocytogenes isolated from production lines of fresh and cold-smoked fish. Journal of Applied Microbiology. ISSN 1365-2672. Vol. 91, n.º 3 (2001), p. 556–562 10.1046/j.1365-2672.2001.01425.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Willey Blackwell |
publisher.none.fl_str_mv |
Willey Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303118452621312 |