Effect of celastrol on bone structure and mechanics in arthritic rats

Detalhes bibliográficos
Autor(a) principal: Cascao, Rita
Data de Publicação: 2017
Outros Autores: Vidal, Bruno, Jalmari Finnilä, Mikko Arttu, Lopes, Inês, Teixeira, Rui Lourenço, Saarakkala, Simo, Moita, Luis Ferreira, Fonseca, João Eurico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/54371
Resumo: © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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spelling Effect of celastrol on bone structure and mechanics in arthritic ratsAdjuvant-induced arthritisBone lossCelastrolInflammationRheumatoid arthritis© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/Objective: Rheumatoid arthritis (RA) is characterised by chronic inflammation leading to articular bone and cartilage damage. Despite recent progress in RA management, adverse effects, lack of efficacy and economic barriers to treatment access still limit therapeutic success. Therefore, safer and less expensive treatments that control inflammation and bone resorption are needed. We have previously shown that celastrol is a candidate for RA treatment. We have observed that it inhibits both interleukin (IL)-1β and tumor necrosis factor (TNF) in vitro, and that it has anti-inflammatory properties and ability to decrease synovial CD68+ macrophages in vivo. Herein our goal was to evaluate the effect of celastrol in local and systemic bone loss. Methods: Celastrol was administrated intraperitoneally at a dose of 1 µg/g/day to female Wistar adjuvant-induced arthritic rats. Rats were sacrificed after 22 days of disease progression, and blood, femurs, tibiae and paw samples were collected for bone remodelling markers quantification, 3-point bending test, micro-CT analysis, nanoindentation and Fourier transform infrared spectroscopy measurements, and immunohistochemical evaluation. Results: We have observed that celastrol preserved articular structures and decreased the number of osteoclasts and osteoblasts present in arthritic joints. Moreover, celastrol reduced tartrate-resistant acid phosphatase 5b, procollagen type 1 amino-terminal propeptide and C terminal crosslinked telopeptide of type II collagen serum levels. Importantly, celastrol prevented bone loss and bone microarchitecture degradation. Celastrol also preserved bone nanoproperties and mineral content. Additionally, animals treated with celastrol had less fragile bones, as depicted by an increase in maximum load and yield displacement. Conclusions: These results suggest that celastrol reduces both bone resorption and cartilage degradation, and preserves bone structural properties.BMJ Publishing GroupRepositório da Universidade de LisboaCascao, RitaVidal, BrunoJalmari Finnilä, Mikko ArttuLopes, InêsTeixeira, Rui LourençoSaarakkala, SimoMoita, Luis FerreiraFonseca, João Eurico2022-09-08T11:13:51Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/54371engRMD Open. 2017 Sep 14;3(2):e00043810.1136/rmdopen-2017-0004382056-5933info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:00:10Zoai:repositorio.ul.pt:10451/54371Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:54.377829Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of celastrol on bone structure and mechanics in arthritic rats
title Effect of celastrol on bone structure and mechanics in arthritic rats
spellingShingle Effect of celastrol on bone structure and mechanics in arthritic rats
Cascao, Rita
Adjuvant-induced arthritis
Bone loss
Celastrol
Inflammation
Rheumatoid arthritis
title_short Effect of celastrol on bone structure and mechanics in arthritic rats
title_full Effect of celastrol on bone structure and mechanics in arthritic rats
title_fullStr Effect of celastrol on bone structure and mechanics in arthritic rats
title_full_unstemmed Effect of celastrol on bone structure and mechanics in arthritic rats
title_sort Effect of celastrol on bone structure and mechanics in arthritic rats
author Cascao, Rita
author_facet Cascao, Rita
Vidal, Bruno
Jalmari Finnilä, Mikko Arttu
Lopes, Inês
Teixeira, Rui Lourenço
Saarakkala, Simo
Moita, Luis Ferreira
Fonseca, João Eurico
author_role author
author2 Vidal, Bruno
Jalmari Finnilä, Mikko Arttu
Lopes, Inês
Teixeira, Rui Lourenço
Saarakkala, Simo
Moita, Luis Ferreira
Fonseca, João Eurico
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Cascao, Rita
Vidal, Bruno
Jalmari Finnilä, Mikko Arttu
Lopes, Inês
Teixeira, Rui Lourenço
Saarakkala, Simo
Moita, Luis Ferreira
Fonseca, João Eurico
dc.subject.por.fl_str_mv Adjuvant-induced arthritis
Bone loss
Celastrol
Inflammation
Rheumatoid arthritis
topic Adjuvant-induced arthritis
Bone loss
Celastrol
Inflammation
Rheumatoid arthritis
description © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2022-09-08T11:13:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/54371
url http://hdl.handle.net/10451/54371
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv RMD Open. 2017 Sep 14;3(2):e000438
10.1136/rmdopen-2017-000438
2056-5933
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMJ Publishing Group
publisher.none.fl_str_mv BMJ Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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