Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/7379 |
Resumo: | The life-threatening mycoses caused by opportunistic fungal pathogens (mainly species from the genus Candida) associated with nosocomial infections, are one of the major health problems in our days. Therefore, it is crucial to identify new compounds, especially natural ones, that are active against the most broaden spectrum of Candida species. Herein, a screening of the antifungal potential of a phenolic extract of Cistus ladanifer from Northeastern Portugal, against Candida species was performed. Furthermore, the extract was characterized by HPLC-DAD-ESI/MS. Phenolic acids and derivatives (3.96 mg/g extract), ellagic acid derivatives (30.34 mg/g extract), and flavonoids (4.15 mg/g extract), such as catechins, flavonols and flavones, were found in the sample. The most abundant group was ellagic acid derivatives in which punicalagin gallate, a derivative of punicalagin attached to gallic acid, was found in highest amount (15.99 ± 0.02 mg/g extract). These compounds (i.e., ellagitannins) could be related to the strong inhibition of C. albicans, C. glabrata and C. parapsilosis growth (MIC < 0.05 mg/ml). Moreover, the best antifungal activity was against C. glabrata, where the studied extract was able to cause at least 3 Log of reduction at concentrations below 0.05 mg/mL and a total growth inhibition at concentrations above 0.625 mg/mL. |
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Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extractsCistus ladaniferPhenolic compoundsHPLC-DAD-ESI/MSCandida speciesAntifungal activityThe life-threatening mycoses caused by opportunistic fungal pathogens (mainly species from the genus Candida) associated with nosocomial infections, are one of the major health problems in our days. Therefore, it is crucial to identify new compounds, especially natural ones, that are active against the most broaden spectrum of Candida species. Herein, a screening of the antifungal potential of a phenolic extract of Cistus ladanifer from Northeastern Portugal, against Candida species was performed. Furthermore, the extract was characterized by HPLC-DAD-ESI/MS. Phenolic acids and derivatives (3.96 mg/g extract), ellagic acid derivatives (30.34 mg/g extract), and flavonoids (4.15 mg/g extract), such as catechins, flavonols and flavones, were found in the sample. The most abundant group was ellagic acid derivatives in which punicalagin gallate, a derivative of punicalagin attached to gallic acid, was found in highest amount (15.99 ± 0.02 mg/g extract). These compounds (i.e., ellagitannins) could be related to the strong inhibition of C. albicans, C. glabrata and C. parapsilosis growth (MIC < 0.05 mg/ml). Moreover, the best antifungal activity was against C. glabrata, where the studied extract was able to cause at least 3 Log of reduction at concentrations below 0.05 mg/mL and a total growth inhibition at concentrations above 0.625 mg/mL.ElsevierBiblioteca Digital do IPBBarros, LillianDueñas, MontserratAlves, Carlos TiagoSilva, SóniaHenriques, MarianaSantos-Buelga, CelestinoFerreira, Isabel C.F.R.2012-08-23T09:19:14Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10198/7379engBarros, Lillian; Dueñas, Montserrat; Alves, Carlos Tiago; Silva, Sónia; Henriques, Mariana; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2012). Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts. Industrial Crops and Products. ISSN 0926-6690. 41, p. 41-450926-669010.1016/j.indcrop.2012.03.038info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:18:13Zoai:bibliotecadigital.ipb.pt:10198/7379Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:59:08.165565Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts |
title |
Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts |
spellingShingle |
Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts Barros, Lillian Cistus ladanifer Phenolic compounds HPLC-DAD-ESI/MS Candida species Antifungal activity |
title_short |
Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts |
title_full |
Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts |
title_fullStr |
Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts |
title_full_unstemmed |
Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts |
title_sort |
Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts |
author |
Barros, Lillian |
author_facet |
Barros, Lillian Dueñas, Montserrat Alves, Carlos Tiago Silva, Sónia Henriques, Mariana Santos-Buelga, Celestino Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Dueñas, Montserrat Alves, Carlos Tiago Silva, Sónia Henriques, Mariana Santos-Buelga, Celestino Ferreira, Isabel C.F.R. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Barros, Lillian Dueñas, Montserrat Alves, Carlos Tiago Silva, Sónia Henriques, Mariana Santos-Buelga, Celestino Ferreira, Isabel C.F.R. |
dc.subject.por.fl_str_mv |
Cistus ladanifer Phenolic compounds HPLC-DAD-ESI/MS Candida species Antifungal activity |
topic |
Cistus ladanifer Phenolic compounds HPLC-DAD-ESI/MS Candida species Antifungal activity |
description |
The life-threatening mycoses caused by opportunistic fungal pathogens (mainly species from the genus Candida) associated with nosocomial infections, are one of the major health problems in our days. Therefore, it is crucial to identify new compounds, especially natural ones, that are active against the most broaden spectrum of Candida species. Herein, a screening of the antifungal potential of a phenolic extract of Cistus ladanifer from Northeastern Portugal, against Candida species was performed. Furthermore, the extract was characterized by HPLC-DAD-ESI/MS. Phenolic acids and derivatives (3.96 mg/g extract), ellagic acid derivatives (30.34 mg/g extract), and flavonoids (4.15 mg/g extract), such as catechins, flavonols and flavones, were found in the sample. The most abundant group was ellagic acid derivatives in which punicalagin gallate, a derivative of punicalagin attached to gallic acid, was found in highest amount (15.99 ± 0.02 mg/g extract). These compounds (i.e., ellagitannins) could be related to the strong inhibition of C. albicans, C. glabrata and C. parapsilosis growth (MIC < 0.05 mg/ml). Moreover, the best antifungal activity was against C. glabrata, where the studied extract was able to cause at least 3 Log of reduction at concentrations below 0.05 mg/mL and a total growth inhibition at concentrations above 0.625 mg/mL. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-23T09:19:14Z 2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/7379 |
url |
http://hdl.handle.net/10198/7379 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Barros, Lillian; Dueñas, Montserrat; Alves, Carlos Tiago; Silva, Sónia; Henriques, Mariana; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2012). Antifungal activity and detailed chemical characterization of Cistus ladanifer phenolic extracts. Industrial Crops and Products. ISSN 0926-6690. 41, p. 41-45 0926-6690 10.1016/j.indcrop.2012.03.038 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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