Orientational behaviors of silk fibroin hydrogels
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/51406 |
Resumo: | In this study, a novel shear-induced silk fibroin hydrogel with three-dimensional (3D) anisotropic and oriented gel skeleton/network morphology is presented. Amphipathic anionic and nontoxic sodium surfactin is blended with the silk fibroin to decrease its gelation time during the mechanical shearing process. The fibroin/surfactin blended solutions undergo a facial shearing process to accomplish a solâ gel transition within one hour. The dynamic solâ gel transition kinetic analysis, gel skeleton/network morphology, and mechanical property measurements are determined in order to visualize the fibroin/surfactin solâ gel transition during the shearing process and its resulting hydrogel. The results demonstrate that there is significant b-sheet assembly from random coil conformations in the fibroin/surfactin blended system during the facile shearing process. The silk fibroin b-sheets further transform into a fibrous large-scale aggregation with orientational and parallel arrangements to the shearing direction. The shear-induced fibroin/ surfactin hydrogel exhibits notable anisotropic and oriented 3D skeleton/network morphology and a significant mechanical compressive strength in proportion to the shearing stress, compared with the control fibroin/surfactin hydrogel undergoing no shearing process. Due to its oriented gel skeleton/network structure and significantly enhanced mechanical properties, the shear-induced fibroin/ surfactin gel may be suitable as a biomaterial in 3D oriented tissue regeneration, including for nerves, the cultivation of bone cells, and the repair of defects in muscle and ligament tissues. |
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Orientational behaviors of silk fibroin hydrogelsBiomaterialsGelsMechanical PropertiesStructure–property relationshipsScience & TechnologyIn this study, a novel shear-induced silk fibroin hydrogel with three-dimensional (3D) anisotropic and oriented gel skeleton/network morphology is presented. Amphipathic anionic and nontoxic sodium surfactin is blended with the silk fibroin to decrease its gelation time during the mechanical shearing process. The fibroin/surfactin blended solutions undergo a facial shearing process to accomplish a solâ gel transition within one hour. The dynamic solâ gel transition kinetic analysis, gel skeleton/network morphology, and mechanical property measurements are determined in order to visualize the fibroin/surfactin solâ gel transition during the shearing process and its resulting hydrogel. The results demonstrate that there is significant b-sheet assembly from random coil conformations in the fibroin/surfactin blended system during the facile shearing process. The silk fibroin b-sheets further transform into a fibrous large-scale aggregation with orientational and parallel arrangements to the shearing direction. The shear-induced fibroin/ surfactin hydrogel exhibits notable anisotropic and oriented 3D skeleton/network morphology and a significant mechanical compressive strength in proportion to the shearing stress, compared with the control fibroin/surfactin hydrogel undergoing no shearing process. Due to its oriented gel skeleton/network structure and significantly enhanced mechanical properties, the shear-induced fibroin/ surfactin gel may be suitable as a biomaterial in 3D oriented tissue regeneration, including for nerves, the cultivation of bone cells, and the repair of defects in muscle and ligament tissues.The work is supported by National Natural Science Foundation of China (Grant No. 51373114), PAPD and College Nature Science Research Project of Jiangsu Province, China (Grant No. 15KJA540001). S. C. Kundu holds ERA Chair Full Professor of European Commission Programme (RoReCaST) at 3Bs Research Group, University of Minho, Portugal.info:eu-repo/semantics/publishedVersionWileyUniversidade do MinhoChen, DaqiYin, ZhupingWu, FengFu, HuaKundu, Subhas CLu, Shenzhou2017-052017-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51406engChen D., Yin Z., Wu F., Fu H., Kundu S. C., Lu S. Orientational behaviors of silk fibroin hydrogels, Journal Of Applied Polymer Science, Vol. 132, Issue 32, doi:10.1002/app.45050, 20170021-89951097-462810.1002/app.45050http://onlinelibrary.wiley.com/doi/10.1002/app.45050/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:20:49ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Orientational behaviors of silk fibroin hydrogels |
title |
Orientational behaviors of silk fibroin hydrogels |
spellingShingle |
Orientational behaviors of silk fibroin hydrogels Chen, Daqi Biomaterials Gels Mechanical Properties Structure–property relationships Science & Technology |
title_short |
Orientational behaviors of silk fibroin hydrogels |
title_full |
Orientational behaviors of silk fibroin hydrogels |
title_fullStr |
Orientational behaviors of silk fibroin hydrogels |
title_full_unstemmed |
Orientational behaviors of silk fibroin hydrogels |
title_sort |
Orientational behaviors of silk fibroin hydrogels |
author |
Chen, Daqi |
author_facet |
Chen, Daqi Yin, Zhuping Wu, Feng Fu, Hua Kundu, Subhas C Lu, Shenzhou |
author_role |
author |
author2 |
Yin, Zhuping Wu, Feng Fu, Hua Kundu, Subhas C Lu, Shenzhou |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Chen, Daqi Yin, Zhuping Wu, Feng Fu, Hua Kundu, Subhas C Lu, Shenzhou |
dc.subject.por.fl_str_mv |
Biomaterials Gels Mechanical Properties Structure–property relationships Science & Technology |
topic |
Biomaterials Gels Mechanical Properties Structure–property relationships Science & Technology |
description |
In this study, a novel shear-induced silk fibroin hydrogel with three-dimensional (3D) anisotropic and oriented gel skeleton/network morphology is presented. Amphipathic anionic and nontoxic sodium surfactin is blended with the silk fibroin to decrease its gelation time during the mechanical shearing process. The fibroin/surfactin blended solutions undergo a facial shearing process to accomplish a solâ gel transition within one hour. The dynamic solâ gel transition kinetic analysis, gel skeleton/network morphology, and mechanical property measurements are determined in order to visualize the fibroin/surfactin solâ gel transition during the shearing process and its resulting hydrogel. The results demonstrate that there is significant b-sheet assembly from random coil conformations in the fibroin/surfactin blended system during the facile shearing process. The silk fibroin b-sheets further transform into a fibrous large-scale aggregation with orientational and parallel arrangements to the shearing direction. The shear-induced fibroin/ surfactin hydrogel exhibits notable anisotropic and oriented 3D skeleton/network morphology and a significant mechanical compressive strength in proportion to the shearing stress, compared with the control fibroin/surfactin hydrogel undergoing no shearing process. Due to its oriented gel skeleton/network structure and significantly enhanced mechanical properties, the shear-induced fibroin/ surfactin gel may be suitable as a biomaterial in 3D oriented tissue regeneration, including for nerves, the cultivation of bone cells, and the repair of defects in muscle and ligament tissues. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05 2017-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/51406 |
url |
http://hdl.handle.net/1822/51406 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chen D., Yin Z., Wu F., Fu H., Kundu S. C., Lu S. Orientational behaviors of silk fibroin hydrogels, Journal Of Applied Polymer Science, Vol. 132, Issue 32, doi:10.1002/app.45050, 2017 0021-8995 1097-4628 10.1002/app.45050 http://onlinelibrary.wiley.com/doi/10.1002/app.45050/abstract |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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repository.mail.fl_str_mv |
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1777303735812227072 |