Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats

Detalhes bibliográficos
Autor(a) principal: Cunha, Ana M.
Data de Publicação: 2017
Outros Autores: Esteves, Madalena Curva, Neves, Sofia Pereira, Borges, Sónia Maria de Sousa, Guimarães, Marco Rafael Machado, Sousa, Nuno, Almeida, Armando, Leite-Almeida, Hugo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/50197
Resumo: Pawedness Trait Test (PaTRaT)-A New Paradigm to Evaluate Paw Preference and Dexterity in Rats
id RCAP_aa50263058edcea1858114741f6ce055
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/50197
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str
spelling Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in ratsmotor preferencelateralityhandednesscognitionimpulsivitymemorybehaviorScience & TechnologyPawedness Trait Test (PaTRaT)-A New Paradigm to Evaluate Paw Preference and Dexterity in RatsIn rodents, dexterity is commonly analyzed in preference paradigms in which animals are given the chance to use either the left or the right front paws to manipulate food. However, paw preference and dexterity at population and individual levels are controversial as results are incongruent across paradigms. We have therefore developed a semi-quantitative method-the pawdeness trait test (PaTRaT)-to evaluate paw preference degree in rats. The PaTRaT consists in a classification system, ranging from +4 to 4 where increasingly positive and negative values reflect the bias for left or right paw use, respectively. Sprague-Dawley male rats were confined into a metal rectangular mesh cylinder, from which they can see, smell and reach sugared rewards with their paws. Due to its size, the reward could only cross the mesh if aligned with its diagonal, imposing additional coordination. Animals were allowed to retrieve 10 rewards per session in a total of four sessions while their behavior was recorded. PaTRaT was repeated 4 and 8 weeks after the first evaluation. To exclude potential bias, rats were also tested for paw fine movement and general locomotion in other behavioral paradigms as well as impulsivity (variable delay-to-signal, VDS), memory and cognitive flexibility (water maze). At the population level 54% of the animals presented a rightward bias. Individually, all animals presented marked side-preferences, >2 and <-2 for left-and right-sided bias, respectively, and this preference was stable across the three evaluations. Inter-rater consistency was very high between two experienced raters and substantial when two additional inexperienced raters were included. Left-and right-biased animals presented no differences in the ability to perform fine movements with any of the forelimbs (staircase) and general locomotor performance. Additionally, these groups performed similarly in executive function and memory tasks. In conclusion, PaTRaT is able to reliably classify rats' pawedness direction and degree.This work has been funded by the European Regional Development Fund (FEDER), through the Competitiveness Factors Operational Programme (COMPETE) and the Northern Portugal Regional Operational Programme (NORTE 2020) under the Portugal 2020 Partnership Agreement (project NORTE-01-0145-FEDER-000023). It was also funded by National funds, through the Foundation for Science and Technology (Fundacao para a Ciencia e a Tecnologia, FCT), under the scope of the projects POCI-01-0145-FEDER-007038 and PTDC/NEU-SCC/5301/2014. Researchers were supported by FCT grant numbers SFRH/BD/109111/2015 (AMC), SFRH/BD/52291/2013 (ME via Inter-University Doctoral Programme in Ageing and Chronic Disease, PhDOC), PD/BD/114120/2015 (SPN via PhDOC), SFRH/BD/89936/2012 (SB), PD/BD/114117/2015 (MRG via PhDOC) and SFRH/BPD/80118/2011 (HL-A).info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoCunha, Ana M.Esteves, Madalena CurvaNeves, Sofia PereiraBorges, Sónia Maria de SousaGuimarães, Marco Rafael MachadoSousa, NunoAlmeida, ArmandoLeite-Almeida, Hugo20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/50197eng1662-51531662-515310.3389/fnbeh.2017.00192https://www.frontiersin.orginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:51ZPortal AgregadorONG
dc.title.none.fl_str_mv Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
title Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
spellingShingle Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
Cunha, Ana M.
motor preference
laterality
handedness
cognition
impulsivity
memory
behavior
Science & Technology
title_short Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
title_full Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
title_fullStr Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
title_full_unstemmed Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
title_sort Pawedness trait test (PaTRaT) : a new paradigm to evaluate paw preference and dexterity in rats
author Cunha, Ana M.
author_facet Cunha, Ana M.
Esteves, Madalena Curva
Neves, Sofia Pereira
Borges, Sónia Maria de Sousa
Guimarães, Marco Rafael Machado
Sousa, Nuno
Almeida, Armando
Leite-Almeida, Hugo
author_role author
author2 Esteves, Madalena Curva
Neves, Sofia Pereira
Borges, Sónia Maria de Sousa
Guimarães, Marco Rafael Machado
Sousa, Nuno
Almeida, Armando
Leite-Almeida, Hugo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Cunha, Ana M.
Esteves, Madalena Curva
Neves, Sofia Pereira
Borges, Sónia Maria de Sousa
Guimarães, Marco Rafael Machado
Sousa, Nuno
Almeida, Armando
Leite-Almeida, Hugo
dc.subject.por.fl_str_mv motor preference
laterality
handedness
cognition
impulsivity
memory
behavior
Science & Technology
topic motor preference
laterality
handedness
cognition
impulsivity
memory
behavior
Science & Technology
description Pawedness Trait Test (PaTRaT)-A New Paradigm to Evaluate Paw Preference and Dexterity in Rats
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/50197
url http://hdl.handle.net/1822/50197
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1662-5153
1662-5153
10.3389/fnbeh.2017.00192
https://www.frontiersin.org
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1777303702788374528

Registros relacionados