Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens

Detalhes bibliográficos
Autor(a) principal: Antunes, Jorge M. A.
Data de Publicação: 2022
Outros Autores: Silva, Marta A., Salgueiro, Carlos A., Morgado, Leonor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143758
Resumo: project LA/P/0140/2020 (i4HB). Publisher Copyright: Copyright © 2022 Antunes, Silva, Salgueiro and Morgado.
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spelling Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducensBiochemical Characterization of Cytochrome CbcLextracellular electron transferGeobacterinner membrane associated cytochromeNMRprotein-protein interactionsMicrobiologyMicrobiology (medical)project LA/P/0140/2020 (i4HB). Publisher Copyright: Copyright © 2022 Antunes, Silva, Salgueiro and Morgado.Exoelectrogenic microorganisms are in the spotlight due to their unique respiratory mechanisms and potential applications in distinct biotechnological fields, including bioremediation, bioenergy production and microbial electrosynthesis. These applications rely on the capability of these microorganisms to perform extracellular electron transfer, a mechanism that allows the bacteria to transfer electrons to the cell’s exterior by establishing functional interfaces between different multiheme cytochromes at the inner membrane, periplasmic space, and outer membrane. The multiheme cytochrome CbcL from Geobacter sulfurreducens is associated to the inner membrane and plays an essential role in the transfer of electrons to final electron acceptors with a low redox potential, as Fe(III) oxides and electrodes poised at −100 mV. CbcL has a transmembranar di-heme b-type cytochrome domain with six helices, linked to a periplasmic cytochrome domain with nine c-type heme groups. The complementary usage of ultraviolet-visible, circular dichroism and nuclear magnetic resonance permitted the structural and functional characterization of CbcL’s periplasmic domain. The protein was found to have a high percentage of disordered regions and its nine hemes are low-spin and all coordinated by two histidine residues. The apparent midpoint reduction potential of the CbcL periplasmic domain was determined, suggesting a thermodynamically favorable transfer of electrons to the putative redox partner in the periplasm − the triheme cytochrome PpcA. The establishment of a redox complex between the two proteins was confirmed by probing the electron transfer reaction and the molecular interactions between CbcL and PpcA. The results obtained show for the first time how electrons are injected into the periplasm of Geobacter sulfurreducens for subsequent transfer to the cell’s exterior.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNAntunes, Jorge M. A.Silva, Marta A.Salgueiro, Carlos A.Morgado, Leonor2022-09-15T22:38:10Z2022-05-102022-05-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/143758eng1664-302XPURE: 46297239https://doi.org/10.3389/fmicb.2022.898015info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:22:28Zoai:run.unl.pt:10362/143758Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:07.616135Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens
Biochemical Characterization of Cytochrome CbcL
title Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens
spellingShingle Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens
Antunes, Jorge M. A.
extracellular electron transfer
Geobacter
inner membrane associated cytochrome
NMR
protein-protein interactions
Microbiology
Microbiology (medical)
title_short Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens
title_full Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens
title_fullStr Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens
title_full_unstemmed Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens
title_sort Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens
author Antunes, Jorge M. A.
author_facet Antunes, Jorge M. A.
Silva, Marta A.
Salgueiro, Carlos A.
Morgado, Leonor
author_role author
author2 Silva, Marta A.
Salgueiro, Carlos A.
Morgado, Leonor
author2_role author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Antunes, Jorge M. A.
Silva, Marta A.
Salgueiro, Carlos A.
Morgado, Leonor
dc.subject.por.fl_str_mv extracellular electron transfer
Geobacter
inner membrane associated cytochrome
NMR
protein-protein interactions
Microbiology
Microbiology (medical)
topic extracellular electron transfer
Geobacter
inner membrane associated cytochrome
NMR
protein-protein interactions
Microbiology
Microbiology (medical)
description project LA/P/0140/2020 (i4HB). Publisher Copyright: Copyright © 2022 Antunes, Silva, Salgueiro and Morgado.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-15T22:38:10Z
2022-05-10
2022-05-10T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143758
url http://hdl.handle.net/10362/143758
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-302X
PURE: 46297239
https://doi.org/10.3389/fmicb.2022.898015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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