An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine

• Autor(a) principal: Hallard, Didier
• Data de Publicação: 1998
• Outros Autores: Heijden, Robert Van Der, Contin, Adriana, Tomaz Jiméréz, Emília M., Snoeijer, Wim, Verpoorte, Robert, Jensen, Soren R., Cardoso, M. Inês Lopes, Pasquali, Giancarlo, Memelink, Johan, Hoge, J. Harry C.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10284/8624
• topic: Nicotiana tabacum; High performance liquid chromatography; Strictosidine synthase; Secoiridoids; Secologanin

The secoiridoid glucoside secologanin is the terpenoid building block in the biosynthesis of terpenoid indole alkaloids. A method for its determination in plant tissues and in cell suspension cultures has been developed. This assay is based on the condensation of secologanin with tryptamine, yielding strictosidine, in a reaction catalysed by the enzyme strictosidine synthase (STR; E.C. 4.3.3.2). Subsequently, the formation of strictosidine is quantified by high performance liquid chromatography (HPLC). STR was isolated from transgenic Nicotiana tabacum cells expressing a cDNA-derived gene coding for STR from Catharanthus roseus. The high specificity of STR for secologanin, in combination with a sensitive and selective HPLC system, allows a simple extraction of secologanin from plant tissue. The detection limit of this method is 15 ng secologanin. Using this assay, secologanin contents were determined in tissues of various plant species; Lonicera xylosteum hairy roots were found to contain 1% of secologanin on a dry weight basis. # 1998 John Wiley & Sons, Ltd.