Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/43723 |
Resumo: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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Assessment of the presence of Hepatitis E virus in surface water and drinking water in PortugalDrinking waterEnteric virusesHepatitis E virusRT-qPCRSurface waterVero E6 cell lineViral infectivityWater qualityWater treatment© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Hepatitis E virus (HEV) is a non-enveloped single-stranded positive-sense RNA virus, belonging to the Hepeviridae family, resistant to environmental conditions, and transmitted by the consumption of contaminated water. This virus is responsible for both sporadic and epidemic outbreaks, leading to thousands of infections per year in several countries, and is thus considered an emerging disease in Europe and Asia. This study refers to a survey in Portugal during 2019, targeting the detection and eventual quantification of enteric viruses in samples from surface and drinking water. Samples positive for HEV RNA were recurrently found by reverse transcription quantitative PCR (RT-qPCR), in both types of matrix. The infectivity of these samples was evaluated in cultured Vero E6 cells and RNA from putative viruses produced in cultures evidencing cytopathic effects and was subjected to RT-qPCR targeting HEV genomic RNA. Our results evidenced the existence of samples positive either for HEV RNA (77.8% in surface water and 66.7% in drinking water) or for infectious HEV (23.0% in surface water and 27.7% in drinking water). These results highlight the need for effective virological control of water for human consumption and activities.This research was funded by Empresa Portuguesa das Águas Livres (EPAL) and Foundation for Science and Technology (FCT) Portugal through a Ph.D. grant to D. Salvador (PDE/BDE/114582/2016) and FCT/MCTES projects UIDP/50017/2020+UIDB/50017/2020 and UIDB/04295/2020 through national funds.MDPIRepositório da Universidade de LisboaSalvador, DanielNeto, CéliaBenoliel, Maria JoãoCaeiro, M. F.2020-05-27T13:28:08Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/43723engMicroorganisms 2020, 8(5), 76110.3390/microorganisms80507612076-2607info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:44:18Zoai:repositorio.ul.pt:10451/43723Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:56:27.342259Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal |
| title |
Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal |
| spellingShingle |
Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal Salvador, Daniel Drinking water Enteric viruses Hepatitis E virus RT-qPCR Surface water Vero E6 cell line Viral infectivity Water quality Water treatment |
| title_short |
Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal |
| title_full |
Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal |
| title_fullStr |
Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal |
| title_full_unstemmed |
Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal |
| title_sort |
Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal |
| author |
Salvador, Daniel |
| author_facet |
Salvador, Daniel Neto, Célia Benoliel, Maria João Caeiro, M. F. |
| author_role |
author |
| author2 |
Neto, Célia Benoliel, Maria João Caeiro, M. F. |
| author2_role |
author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Salvador, Daniel Neto, Célia Benoliel, Maria João Caeiro, M. F. |
| dc.subject.por.fl_str_mv |
Drinking water Enteric viruses Hepatitis E virus RT-qPCR Surface water Vero E6 cell line Viral infectivity Water quality Water treatment |
| topic |
Drinking water Enteric viruses Hepatitis E virus RT-qPCR Surface water Vero E6 cell line Viral infectivity Water quality Water treatment |
| description |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-05-27T13:28:08Z 2020 2020-01-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/43723 |
| url |
http://hdl.handle.net/10451/43723 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Microorganisms 2020, 8(5), 761 10.3390/microorganisms8050761 2076-2607 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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