Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/27701 |
Resumo: | Immunoglobulin G (IgG) has been used in the treatment of cancer, autoimmune diseases and neurological disorders, however, the current technologies to purify and recover IgG from biological media are of high-cost and time-consuming, resulting in high-cost products. In this sense, the search for cost-effective technologies to obtain highly pure and active IgG is highly required. The present work proposes a simple and efficient method for the purification and recovery of IgG from rabbit serum using magnetic iron oxide nanoparticles (magnetite, Fe3O4) coated with hybrid shells of a siliceous material modified with the anionic polysaccharide κ-carrageenan. Experimental parameters such as pH, contact time between the hybrid magnetic nanoparticles (HMNPs) and rabbit serum, and total protein concentration or dilution factor of serum were evaluated. The best results were achieved at pH 5.0, with a contact time of 60 min and using a rabbit serum with a total protein concentration of 4.8 mg·mL−1. Under these conditions, it was obtained an IgG purification factor and adsorption yield onto the HMNPs of 3.0 and 90%, respectively. The desorption of IgG from the HMNPs was evaluated using two strategies: a KCl aqueous solution and buffered aqueous solutions. Comparing to the initial rabbit serum, an IgG purification factor of 2.7 with a recovery yield of 74% were obtained using a buffered aqueous solution at pH 7.0. After desorption, the secondary structure of IgG and other proteins was evaluated by circular dichroism and no changes in the secondary structure were observed, meaning that the IgG integrity is kept after the adsorption and desorption steps. In summary, the application of HMNPs in the purification of IgG from serum samples has a high potential as a new downstream platform. |
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Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticlesImmunoglobulin GAdsorptionk-CarrageenanMagnetic nanoparticlesRecoveryImmunoglobulin G (IgG) has been used in the treatment of cancer, autoimmune diseases and neurological disorders, however, the current technologies to purify and recover IgG from biological media are of high-cost and time-consuming, resulting in high-cost products. In this sense, the search for cost-effective technologies to obtain highly pure and active IgG is highly required. The present work proposes a simple and efficient method for the purification and recovery of IgG from rabbit serum using magnetic iron oxide nanoparticles (magnetite, Fe3O4) coated with hybrid shells of a siliceous material modified with the anionic polysaccharide κ-carrageenan. Experimental parameters such as pH, contact time between the hybrid magnetic nanoparticles (HMNPs) and rabbit serum, and total protein concentration or dilution factor of serum were evaluated. The best results were achieved at pH 5.0, with a contact time of 60 min and using a rabbit serum with a total protein concentration of 4.8 mg·mL−1. Under these conditions, it was obtained an IgG purification factor and adsorption yield onto the HMNPs of 3.0 and 90%, respectively. The desorption of IgG from the HMNPs was evaluated using two strategies: a KCl aqueous solution and buffered aqueous solutions. Comparing to the initial rabbit serum, an IgG purification factor of 2.7 with a recovery yield of 74% were obtained using a buffered aqueous solution at pH 7.0. After desorption, the secondary structure of IgG and other proteins was evaluated by circular dichroism and no changes in the secondary structure were observed, meaning that the IgG integrity is kept after the adsorption and desorption steps. In summary, the application of HMNPs in the purification of IgG from serum samples has a high potential as a new downstream platform.Elsevier2021-05-01T00:00:00Z2020-05-01T00:00:00Z2020-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/27701eng0141-813010.1016/j.ijbiomac.2020.02.135Magalhães, Flávia F.Almeida, Mafalda R.Soares, Sofia F.Trindade, TitoFreire, Mara G.Daniel-da-Silva, Ana LuísaTavares, Ana P. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:53:31Zoai:ria.ua.pt:10773/27701Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:00:21.218722Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles |
title |
Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles |
spellingShingle |
Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles Magalhães, Flávia F. Immunoglobulin G Adsorption k-Carrageenan Magnetic nanoparticles Recovery |
title_short |
Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles |
title_full |
Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles |
title_fullStr |
Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles |
title_full_unstemmed |
Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles |
title_sort |
Recovery of immunoglobulin G from rabbit serum using k-carrageenan-modified hybrid magnetic nanoparticles |
author |
Magalhães, Flávia F. |
author_facet |
Magalhães, Flávia F. Almeida, Mafalda R. Soares, Sofia F. Trindade, Tito Freire, Mara G. Daniel-da-Silva, Ana Luísa Tavares, Ana P. M. |
author_role |
author |
author2 |
Almeida, Mafalda R. Soares, Sofia F. Trindade, Tito Freire, Mara G. Daniel-da-Silva, Ana Luísa Tavares, Ana P. M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Magalhães, Flávia F. Almeida, Mafalda R. Soares, Sofia F. Trindade, Tito Freire, Mara G. Daniel-da-Silva, Ana Luísa Tavares, Ana P. M. |
dc.subject.por.fl_str_mv |
Immunoglobulin G Adsorption k-Carrageenan Magnetic nanoparticles Recovery |
topic |
Immunoglobulin G Adsorption k-Carrageenan Magnetic nanoparticles Recovery |
description |
Immunoglobulin G (IgG) has been used in the treatment of cancer, autoimmune diseases and neurological disorders, however, the current technologies to purify and recover IgG from biological media are of high-cost and time-consuming, resulting in high-cost products. In this sense, the search for cost-effective technologies to obtain highly pure and active IgG is highly required. The present work proposes a simple and efficient method for the purification and recovery of IgG from rabbit serum using magnetic iron oxide nanoparticles (magnetite, Fe3O4) coated with hybrid shells of a siliceous material modified with the anionic polysaccharide κ-carrageenan. Experimental parameters such as pH, contact time between the hybrid magnetic nanoparticles (HMNPs) and rabbit serum, and total protein concentration or dilution factor of serum were evaluated. The best results were achieved at pH 5.0, with a contact time of 60 min and using a rabbit serum with a total protein concentration of 4.8 mg·mL−1. Under these conditions, it was obtained an IgG purification factor and adsorption yield onto the HMNPs of 3.0 and 90%, respectively. The desorption of IgG from the HMNPs was evaluated using two strategies: a KCl aqueous solution and buffered aqueous solutions. Comparing to the initial rabbit serum, an IgG purification factor of 2.7 with a recovery yield of 74% were obtained using a buffered aqueous solution at pH 7.0. After desorption, the secondary structure of IgG and other proteins was evaluated by circular dichroism and no changes in the secondary structure were observed, meaning that the IgG integrity is kept after the adsorption and desorption steps. In summary, the application of HMNPs in the purification of IgG from serum samples has a high potential as a new downstream platform. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-01T00:00:00Z 2020-05-01 2021-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/27701 |
url |
http://hdl.handle.net/10773/27701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0141-8130 10.1016/j.ijbiomac.2020.02.135 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137658567393280 |