Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals

Detalhes bibliográficos
Autor(a) principal: Vieira, Vanessa
Data de Publicação: 2018
Outros Autores: Pereira, Carla, Abreu, Rui M.V., Calhelha, Ricardo C., Alves, Maria José, Coutinho, João A.P., Ferreira, Olga, Barros, Lillian, Ferreira, Isabel C.F.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/22319
Resumo: Juglans regia L. (walnut) green husks are an important fraction of waste resulting from the walnut production, thus representing an interesting natural matrix to explore as a source of bioactive compounds. In this work, the hydroethanolic extract of walnut green husks was studied considering the phytochemical composition and the biological activity using different cell model assays, most of them evaluated for the first time for this matrix. From the HPLC-DAD-ESI/MSn analysis, sixteen compounds were identified, being the extract mostly composed of naphthalene derivatives (including tetralone derivatives) and less abundant in phenolic compounds (hydroxycinnamic acids and flavonols). The cytotoxic potential of the extract was assessed against tumour (MCF- 7, NCI–H460, HeLa and HepG2) and non-tumour (PLP2) cell lines. Moreover, the antioxidant activity of the extract was evaluated by inhibition of the oxidative haemolysis (OxHLIA) and the formation of thiobarbituric acid reactive substances (TBARS), and the anti-inflammatory potential by the inhibition of the NO production by the RAW264.7 cell culture. The antibacterial effects of the extract were also evaluated against Gram-negative and Gram-positive bacteria. The results obtained represent a stepping stone for the development of future applications using walnut green husks as a source of added value compounds with bioactive potential.
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spelling Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicalsAnti-inflammatoryAntibacterialAntioxidantCytotoxicityJuglans regia L. husksNaphthalene derivativesPhenolic compoundsJuglans regia L. (walnut) green husks are an important fraction of waste resulting from the walnut production, thus representing an interesting natural matrix to explore as a source of bioactive compounds. In this work, the hydroethanolic extract of walnut green husks was studied considering the phytochemical composition and the biological activity using different cell model assays, most of them evaluated for the first time for this matrix. From the HPLC-DAD-ESI/MSn analysis, sixteen compounds were identified, being the extract mostly composed of naphthalene derivatives (including tetralone derivatives) and less abundant in phenolic compounds (hydroxycinnamic acids and flavonols). The cytotoxic potential of the extract was assessed against tumour (MCF- 7, NCI–H460, HeLa and HepG2) and non-tumour (PLP2) cell lines. Moreover, the antioxidant activity of the extract was evaluated by inhibition of the oxidative haemolysis (OxHLIA) and the formation of thiobarbituric acid reactive substances (TBARS), and the anti-inflammatory potential by the inhibition of the NO production by the RAW264.7 cell culture. The antibacterial effects of the extract were also evaluated against Gram-negative and Gram-positive bacteria. The results obtained represent a stepping stone for the development of future applications using walnut green husks as a source of added value compounds with bioactive potential.The authors thank the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019) and V. Vieira grant (SFRH/BD/ 108487/2015); national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros and R. Calhelha contracts, and C. Pereira contract celebrated though the program-contract foreseen in No. 4, 5 and 6 of article 23° of Decree-Law No. 57/ 2016, of 29th August, amended by Law No. 57/2017, of 19th July. To UID/CTM/50011/2019 (CICECO), financed by national funds through the FCT/MCTES. To POCI-01-0145-FEDER-006984 (LA LSRE-LCM), funded by ERDF, through POCI-COMPETE2020 and FCT. To project AllNat - POCI-01-0145-FEDER-030463 (PTDC/EQU-EPQ/30463/ 2017), funded by FEDER funds through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI), and by national funds through Foundation for Science and Technology (FCT/ MCTES), and to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.Biblioteca Digital do IPBVieira, VanessaPereira, CarlaAbreu, Rui M.V.Calhelha, Ricardo C.Alves, Maria JoséCoutinho, João A.P.Ferreira, OlgaBarros, LillianFerreira, Isabel C.F.R.2018-01-19T10:00:00Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/22319engVieira, Vanessa; Pereira, Carla; Abreu, Rui M.V.; Calhelha, Ricardo C.; Alves, Maria José; Coutinho, João A.P.; Ferreira, Olga; Barros, Lillian; Ferreira, Isabel C.F.R. (2020). Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals. Food and Chemical Toxicology. ISSN 0278-6915. 137, p. 1-80278-691510.1016/j.fct.2020.111189info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:50:03Zoai:bibliotecadigital.ipb.pt:10198/22319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:13:34.162084Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
title Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
spellingShingle Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
Vieira, Vanessa
Anti-inflammatory
Antibacterial
Antioxidant
Cytotoxicity
Juglans regia L. husks
Naphthalene derivatives
Phenolic compounds
title_short Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
title_full Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
title_fullStr Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
title_full_unstemmed Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
title_sort Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
author Vieira, Vanessa
author_facet Vieira, Vanessa
Pereira, Carla
Abreu, Rui M.V.
Calhelha, Ricardo C.
Alves, Maria José
Coutinho, João A.P.
Ferreira, Olga
Barros, Lillian
Ferreira, Isabel C.F.R.
author_role author
author2 Pereira, Carla
Abreu, Rui M.V.
Calhelha, Ricardo C.
Alves, Maria José
Coutinho, João A.P.
Ferreira, Olga
Barros, Lillian
Ferreira, Isabel C.F.R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Vieira, Vanessa
Pereira, Carla
Abreu, Rui M.V.
Calhelha, Ricardo C.
Alves, Maria José
Coutinho, João A.P.
Ferreira, Olga
Barros, Lillian
Ferreira, Isabel C.F.R.
dc.subject.por.fl_str_mv Anti-inflammatory
Antibacterial
Antioxidant
Cytotoxicity
Juglans regia L. husks
Naphthalene derivatives
Phenolic compounds
topic Anti-inflammatory
Antibacterial
Antioxidant
Cytotoxicity
Juglans regia L. husks
Naphthalene derivatives
Phenolic compounds
description Juglans regia L. (walnut) green husks are an important fraction of waste resulting from the walnut production, thus representing an interesting natural matrix to explore as a source of bioactive compounds. In this work, the hydroethanolic extract of walnut green husks was studied considering the phytochemical composition and the biological activity using different cell model assays, most of them evaluated for the first time for this matrix. From the HPLC-DAD-ESI/MSn analysis, sixteen compounds were identified, being the extract mostly composed of naphthalene derivatives (including tetralone derivatives) and less abundant in phenolic compounds (hydroxycinnamic acids and flavonols). The cytotoxic potential of the extract was assessed against tumour (MCF- 7, NCI–H460, HeLa and HepG2) and non-tumour (PLP2) cell lines. Moreover, the antioxidant activity of the extract was evaluated by inhibition of the oxidative haemolysis (OxHLIA) and the formation of thiobarbituric acid reactive substances (TBARS), and the anti-inflammatory potential by the inhibition of the NO production by the RAW264.7 cell culture. The antibacterial effects of the extract were also evaluated against Gram-negative and Gram-positive bacteria. The results obtained represent a stepping stone for the development of future applications using walnut green husks as a source of added value compounds with bioactive potential.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-19T10:00:00Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/22319
url http://hdl.handle.net/10198/22319
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vieira, Vanessa; Pereira, Carla; Abreu, Rui M.V.; Calhelha, Ricardo C.; Alves, Maria José; Coutinho, João A.P.; Ferreira, Olga; Barros, Lillian; Ferreira, Isabel C.F.R. (2020). Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals. Food and Chemical Toxicology. ISSN 0278-6915. 137, p. 1-8
0278-6915
10.1016/j.fct.2020.111189
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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