Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/44012 |
Resumo: | Currently, valuable biomolecules are sustainably produced through fermentation using genetically modified microorganisms. However, this industry also generates several waste streams, such as spent yeast (SY). To close the sustainability loop in bioproduction, it is essential to define valorization strategies for such waste streams. Hence, this study aimed to investigate the compositional differences among these novel SYs, intending to pave the way for devising highly effective and beneficial valorization strategies for these streams. In this work, SYs derived from the production of beer (SBY) and six biomolecules [β-farnesene (Fene), rebaudioside M (Reb M), manool (MN), patchouli (PAT), sandalwood (SDW), and sclareol (SCL)] through fermentations driven by engineered yeasts were characterized. The results showed that SY from Fene, MN, and PAT production have similar concentrations of protein (35.9–44% dw), minerals, and amino acids, being compatible with bulking and use as nitrogen sources. Those derived from Reb M production showed significantly higher levels of lipids (21 ± 2.6% dw) compared to all the other SYs (1.7–10.8% dw), whereas the ones derived from SCL production showed significantly higher levels of inorganic matter (45%) compared with those found in the other SYs (1.2–11.3% dw). The results showed that assessing the extent of compositional variation among different SYs derived from precision fermentations represents a primary step toward developing efficient valorization or biorefining strategies. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomyBiomass compositionBiorefiningFermentationSaccharomyces cerevisiaeSpent yeastWaste valorizationCurrently, valuable biomolecules are sustainably produced through fermentation using genetically modified microorganisms. However, this industry also generates several waste streams, such as spent yeast (SY). To close the sustainability loop in bioproduction, it is essential to define valorization strategies for such waste streams. Hence, this study aimed to investigate the compositional differences among these novel SYs, intending to pave the way for devising highly effective and beneficial valorization strategies for these streams. In this work, SYs derived from the production of beer (SBY) and six biomolecules [β-farnesene (Fene), rebaudioside M (Reb M), manool (MN), patchouli (PAT), sandalwood (SDW), and sclareol (SCL)] through fermentations driven by engineered yeasts were characterized. The results showed that SY from Fene, MN, and PAT production have similar concentrations of protein (35.9–44% dw), minerals, and amino acids, being compatible with bulking and use as nitrogen sources. Those derived from Reb M production showed significantly higher levels of lipids (21 ± 2.6% dw) compared to all the other SYs (1.7–10.8% dw), whereas the ones derived from SCL production showed significantly higher levels of inorganic matter (45%) compared with those found in the other SYs (1.2–11.3% dw). The results showed that assessing the extent of compositional variation among different SYs derived from precision fermentations represents a primary step toward developing efficient valorization or biorefining strategies.Veritati - Repositório Institucional da Universidade Católica PortuguesaDeuchande, TeresaFundo, Joana F.Rodrigues, DanielaPintado, Manuela E.Amaro, Ana L.2024-02-19T10:46:17Z20242024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/44012eng1877-264110.1007/s12649-023-02394-z85183750890001151700400001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-20T01:33:16Zoai:repositorio.ucp.pt:10400.14/44012Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:39:03.810755Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy |
title |
Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy |
spellingShingle |
Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy Deuchande, Teresa Biomass composition Biorefining Fermentation Saccharomyces cerevisiae Spent yeast Waste valorization |
title_short |
Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy |
title_full |
Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy |
title_fullStr |
Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy |
title_full_unstemmed |
Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy |
title_sort |
Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomy |
author |
Deuchande, Teresa |
author_facet |
Deuchande, Teresa Fundo, Joana F. Rodrigues, Daniela Pintado, Manuela E. Amaro, Ana L. |
author_role |
author |
author2 |
Fundo, Joana F. Rodrigues, Daniela Pintado, Manuela E. Amaro, Ana L. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Deuchande, Teresa Fundo, Joana F. Rodrigues, Daniela Pintado, Manuela E. Amaro, Ana L. |
dc.subject.por.fl_str_mv |
Biomass composition Biorefining Fermentation Saccharomyces cerevisiae Spent yeast Waste valorization |
topic |
Biomass composition Biorefining Fermentation Saccharomyces cerevisiae Spent yeast Waste valorization |
description |
Currently, valuable biomolecules are sustainably produced through fermentation using genetically modified microorganisms. However, this industry also generates several waste streams, such as spent yeast (SY). To close the sustainability loop in bioproduction, it is essential to define valorization strategies for such waste streams. Hence, this study aimed to investigate the compositional differences among these novel SYs, intending to pave the way for devising highly effective and beneficial valorization strategies for these streams. In this work, SYs derived from the production of beer (SBY) and six biomolecules [β-farnesene (Fene), rebaudioside M (Reb M), manool (MN), patchouli (PAT), sandalwood (SDW), and sclareol (SCL)] through fermentations driven by engineered yeasts were characterized. The results showed that SY from Fene, MN, and PAT production have similar concentrations of protein (35.9–44% dw), minerals, and amino acids, being compatible with bulking and use as nitrogen sources. Those derived from Reb M production showed significantly higher levels of lipids (21 ± 2.6% dw) compared to all the other SYs (1.7–10.8% dw), whereas the ones derived from SCL production showed significantly higher levels of inorganic matter (45%) compared with those found in the other SYs (1.2–11.3% dw). The results showed that assessing the extent of compositional variation among different SYs derived from precision fermentations represents a primary step toward developing efficient valorization or biorefining strategies. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02-19T10:46:17Z 2024 2024-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/44012 |
url |
http://hdl.handle.net/10400.14/44012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1877-2641 10.1007/s12649-023-02394-z 85183750890 001151700400001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137440722583552 |