Development, cytotoxicity and eye irritation profile of a new sunscreen formulation based on benzophenone-3-poly(-caprolactone) nanocapsules

• Autor(a) principal: Barbosa, Thallysson Carvalho
• Data de Publicação: 2019
• Outros Autores: Nascimento, Lívia Éven Dias, Bani, Cristiane, Almeida, Taline, Nery, Marcelo, Santos, Rafael Silva, Menezes, Luana Renyelle de Oliveira, Zielinska, Aleksandra, Fernandes, Ana Rita, Cardoso, Juliana Cordeiro, Jäguer, Alessandro, Jäguer, Eliezer, Sanchez-Lopez, Elena, Nalone, Luciana, Souto, Eliana B., Severino, Patrícia
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/61697
• topic: polymeric nanocapsules; poly(e-caprolactone); benzophenone-3; sunscreen; cytotoxicity; ocular tolerance; poly(epsilon-caprolactone); Poly(ε-caprolactone); Science & Technology

The objective of this work was to characterize the toxicological profile of a newly developed sunscreen formulation based on polymeric nanocapsules (NCs) loading benzophenone-3 (BZP3). NCs composed of poly(ε-caprolactone) carrot oil and Pluronic® F68 were produced by emulsification-diffusion method. Their mean particle size (Z-Ave) ranged from 280 to 420 nm, polydispersity index (PDI) was below 0.37, while zeta potential (ZP) reached about |+11 mV|. No cytotoxic effects were observed in L929 fibroblast cell line for the blank (i.e., non-loaded) NCs and BZP3-loaded NCs (BZP3-NCs). The semi-solid sunscreen formulation was stable over time (centrifugation testing) and exhibited non-Newtonian pseudoplastic behavior, which is typical of products for topical application onto the skin. The sun protection factor (SPF) value reached 8.84, when incorporating BZP3-NCs (SPF of 8.64) into the semi-solid formulation. A synergistic effect was also observed when combining the formulation ingredients of nanocapsules, i.e., SPF of carrot oil was 6.82, blank NCs was 6.84, and BZP3-loaded NCs was 8.64. From the hen’s egg-chorioallantoic membrane test (HET-CAM) test, the non-irritation profile of the developed formulations could also be confirmed. The obtained results show a promising use of poly(ε-caprolactone) nanocapsules to be loaded with lipophilic sunscreens as benzophenone-3.