Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection
Autor(a) principal: | |
---|---|
Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/10526 https://doi.org/10.1021/jf050065r |
Resumo: | An analytical method for the determination of OTC and TC residues in honey was developed. Sample treatment involves an extraction in EDTA-McIlvaine buffer, followed by a solid-phase cleanup step. With regard to the cleanup procedure, different SPE cartridges were evaluated and the results presented. The method was validated according to the guidelines laid down by the 2002/657/EC European Decision parameters: decision limit (Ccα) and detection capability (CCβ) were 20 and 21 μg/Kg and 49 and 50 μg/Kg for OTC and TC, respectively, and recoveries of OTC and TC from spiked samples, at three fortification levels, were higher than 87% for both compounds. The analytical method was applied to 57 honey samples. |
id |
RCAP_af60258a6ee25f6b755ab0fe69003287 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/10526 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
|
spelling |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence DetectionAntibioticsTetracyclinesHoneySolid-phase extractionHigh-performance liquid chromatographyAn analytical method for the determination of OTC and TC residues in honey was developed. Sample treatment involves an extraction in EDTA-McIlvaine buffer, followed by a solid-phase cleanup step. With regard to the cleanup procedure, different SPE cartridges were evaluated and the results presented. The method was validated according to the guidelines laid down by the 2002/657/EC European Decision parameters: decision limit (Ccα) and detection capability (CCβ) were 20 and 21 μg/Kg and 49 and 50 μg/Kg for OTC and TC, respectively, and recoveries of OTC and TC from spiked samples, at three fortification levels, were higher than 87% for both compounds. The analytical method was applied to 57 honey samples.American Chemical Society2005-05-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10526http://hdl.handle.net/10316/10526https://doi.org/10.1021/jf050065rengJournal of Agricultural and Food Chemistry. 53:10 (2005) 3784-37880021-8561Pena, A.Pelantova, N.Lino, C. M.Silveira, M. I. N.Solich, P.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-28T10:20:26ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection |
title |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection |
spellingShingle |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection Pena, A. Antibiotics Tetracyclines Honey Solid-phase extraction High-performance liquid chromatography |
title_short |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection |
title_full |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection |
title_fullStr |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection |
title_full_unstemmed |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection |
title_sort |
Validation of an Analytical Methodology for Determination of Oxytetracycline and Tetracycline Residues in Honey by HPLC with Fluorescence Detection |
author |
Pena, A. |
author_facet |
Pena, A. Pelantova, N. Lino, C. M. Silveira, M. I. N. Solich, P. |
author_role |
author |
author2 |
Pelantova, N. Lino, C. M. Silveira, M. I. N. Solich, P. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pena, A. Pelantova, N. Lino, C. M. Silveira, M. I. N. Solich, P. |
dc.subject.por.fl_str_mv |
Antibiotics Tetracyclines Honey Solid-phase extraction High-performance liquid chromatography |
topic |
Antibiotics Tetracyclines Honey Solid-phase extraction High-performance liquid chromatography |
description |
An analytical method for the determination of OTC and TC residues in honey was developed. Sample treatment involves an extraction in EDTA-McIlvaine buffer, followed by a solid-phase cleanup step. With regard to the cleanup procedure, different SPE cartridges were evaluated and the results presented. The method was validated according to the guidelines laid down by the 2002/657/EC European Decision parameters: decision limit (Ccα) and detection capability (CCβ) were 20 and 21 μg/Kg and 49 and 50 μg/Kg for OTC and TC, respectively, and recoveries of OTC and TC from spiked samples, at three fortification levels, were higher than 87% for both compounds. The analytical method was applied to 57 honey samples. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-05-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10526 http://hdl.handle.net/10316/10526 https://doi.org/10.1021/jf050065r |
url |
http://hdl.handle.net/10316/10526 https://doi.org/10.1021/jf050065r |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Agricultural and Food Chemistry. 53:10 (2005) 3784-3788 0021-8561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1777302613900918784 |