Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

Detalhes bibliográficos
Autor(a) principal: Pereira, Hugo
Data de Publicação: 2011
Outros Autores: Barreira, Luísa, Mozes, Andre, Florindo, C., Polo, Cristina, Duarte, Catarina V., Custódio, Luísa, Varela, J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/917
Resumo: Abstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.
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spelling Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgaeAbstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.SapientiaPereira, HugoBarreira, LuísaMozes, AndreFlorindo, C.Polo, CristinaDuarte, Catarina V.Custódio, LuísaVarela, J.2012-02-29T23:36:36Z2011-12-222012-02-27T16:07:00Z2011-12-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/xmlapplication/pdfhttp://hdl.handle.net/10400.1/917engengBiotechnology for Biofuels. 2011 Dec 22;4(1):61AUT: LBA00541; JVA01305;http://dx.doi.org/10.1186/1754-6834-4-61info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:11:47Zoai:sapientia.ualg.pt:10400.1/917Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:55:11.517167Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
spellingShingle Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
Pereira, Hugo
title_short Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_full Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_fullStr Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_full_unstemmed Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
title_sort Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae
author Pereira, Hugo
author_facet Pereira, Hugo
Barreira, Luísa
Mozes, Andre
Florindo, C.
Polo, Cristina
Duarte, Catarina V.
Custódio, Luísa
Varela, J.
author_role author
author2 Barreira, Luísa
Mozes, Andre
Florindo, C.
Polo, Cristina
Duarte, Catarina V.
Custódio, Luísa
Varela, J.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Pereira, Hugo
Barreira, Luísa
Mozes, Andre
Florindo, C.
Polo, Cristina
Duarte, Catarina V.
Custódio, Luísa
Varela, J.
description Abstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-22
2011-12-22T00:00:00Z
2012-02-29T23:36:36Z
2012-02-27T16:07:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/917
url http://hdl.handle.net/10400.1/917
dc.language.iso.fl_str_mv eng
eng
language eng
dc.relation.none.fl_str_mv Biotechnology for Biofuels. 2011 Dec 22;4(1):61
AUT: LBA00541; JVA01305;
http://dx.doi.org/10.1186/1754-6834-4-61
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instacron:RCAAP
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