Reproducing stone monument photosynthetic-based colonization under laboratory conditions

Detalhes bibliográficos
Autor(a) principal: Macedo, Maria Filomena
Data de Publicação: 2008
Outros Autores: Miller, Ana Zélia, Laiz, Leonila, Miguel Gonzalez, Juan, Dionísio, Maria Amélia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/4468
Resumo: Science of the total environment 405(2008) 278-285
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spelling Reproducing stone monument photosynthetic-based colonization under laboratory conditionsBiodeteriorationLaboratory chamberLimestonePhototrophic microorganismsColonization experimentScience of the total environment 405(2008) 278-285In order to understand the biodeterioration process occurring on stone monuments, we analyzed the microbial communities involved in these processes and studied their ability to colonize stones under controlled laboratory experiments. In this study, a natural green biofilm from a limestone monument was cultivated, inoculated on stone probes of the same lithotype and incubated in a laboratory chamber. This incubation system, which exposes stone samples to intermittently sprinkling water, allowed the development of photosynthetic biofilms similar to those occurring on stone monuments. Denaturing gradient gel electrophoresis (DGGE) analysis was used to evaluate the major microbial components of the laboratory biofilms. Cyanobacteria, green microalgae, bacteria and fungi were identified by DNA-based molecular analysis targeting the 16S and 18S ribosomal RNA genes. The natural green biofilm was mainly composed by the Chlorophyta Chlorella, Stichococcus, and Trebouxia, and by Cyanobacteria belonging to the genera Leptolyngbya and Pleurocapsa. A number of bacteria belonging to Alphaproteobacteria, Bacteroidetes and Verrucomicrobia were identified, as well as fungi from the Ascomycota. The laboratory colonization experiment on stone probes showed a colonization pattern similar to that occurring on stone monuments. The methodology described in this paper allowed to reproduce a colonization equivalent to the natural biodeteriorating process.Elsevier Ltd.RUNMacedo, Maria FilomenaMiller, Ana ZéliaLaiz, LeonilaMiguel Gonzalez, JuanDionísio, Maria Amélia2010-12-10T15:47:31Z2008-112008-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/4468eng0048-9697info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:34:20Zoai:run.unl.pt:10362/4468Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:15:43.017196Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Reproducing stone monument photosynthetic-based colonization under laboratory conditions
title Reproducing stone monument photosynthetic-based colonization under laboratory conditions
spellingShingle Reproducing stone monument photosynthetic-based colonization under laboratory conditions
Macedo, Maria Filomena
Biodeterioration
Laboratory chamber
Limestone
Phototrophic microorganisms
Colonization experiment
title_short Reproducing stone monument photosynthetic-based colonization under laboratory conditions
title_full Reproducing stone monument photosynthetic-based colonization under laboratory conditions
title_fullStr Reproducing stone monument photosynthetic-based colonization under laboratory conditions
title_full_unstemmed Reproducing stone monument photosynthetic-based colonization under laboratory conditions
title_sort Reproducing stone monument photosynthetic-based colonization under laboratory conditions
author Macedo, Maria Filomena
author_facet Macedo, Maria Filomena
Miller, Ana Zélia
Laiz, Leonila
Miguel Gonzalez, Juan
Dionísio, Maria Amélia
author_role author
author2 Miller, Ana Zélia
Laiz, Leonila
Miguel Gonzalez, Juan
Dionísio, Maria Amélia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Macedo, Maria Filomena
Miller, Ana Zélia
Laiz, Leonila
Miguel Gonzalez, Juan
Dionísio, Maria Amélia
dc.subject.por.fl_str_mv Biodeterioration
Laboratory chamber
Limestone
Phototrophic microorganisms
Colonization experiment
topic Biodeterioration
Laboratory chamber
Limestone
Phototrophic microorganisms
Colonization experiment
description Science of the total environment 405(2008) 278-285
publishDate 2008
dc.date.none.fl_str_mv 2008-11
2008-11-01T00:00:00Z
2010-12-10T15:47:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/4468
url http://hdl.handle.net/10362/4468
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0048-9697
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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