BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds

Bibliographic Details
Main Author: Ribeiro, Artur J.
Publication Date: 2016
Other Authors: Volkov, Vadim, Oliveira, Mariana Braga, Padrão, Jorge, Mano, J. F., Gomes, Andreia C, Cavaco-Paulo, Artur
Format: Article
Language: eng
Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Download full: https://hdl.handle.net/1822/41808
Summary: "Available online 6 May 2016"
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spelling BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffoldsTunable scaffoldsHydrophobic/Hydrophilic ratioInduced cold gelationSurface wettabilityMechanical propertiesCell proliferationScience & Technology"Available online 6 May 2016"An effective tissue engineering approach requires adjustment according to the target tissue to be engineered. The possibility of obtaining a protein-based formulation for the development of multivalent tunable scaffolds that can be adapted for several types of cells and tissues is explored in this work. The incremental substitution of bovine serum albumin (BSA) by human serum albumin (HSA), changing the scaffolds hydrophilic/hydrophobic ratio, on a previously optimized scaffold formulation resulted in a set of uniform porous scaffolds with different physical properties and associated cell proliferation profile along time. There was a general trend towards an increase in hydrophilicity, swelling degree and in vitro degradation of the scaffolds with increasing replacement of BSA by HAS. The set of BSA/HSA scaffolds presented distinct values for the storage (elastic) modulus and loss factor which were similar to those described for different native tissues such as bone, cartilage, muscle, skin and neural tissue. The preferential adhesion and proliferation of skin fibroblasts on the BSA25%HSA75% and HSA100% scaffolds, as predicted by their viscoelastic properties, demonstrate that the BSA/HSA scaffold formulation is promising for the development of scaffolds that can be tuned according to the tissue to be repaired and restored.This work was supported by FCT I.P through the strategic funding UID/BIO/04469/2013 and the strategic funding UID/BIA/04050/2013. Artur Ribeiro thanks FCT for the SFRHBPD983882013 grant.ElsevierUniversidade do MinhoRibeiro, Artur J.Volkov, VadimOliveira, Mariana BragaPadrão, JorgeMano, J. F.Gomes, Andreia CCavaco-Paulo, Artur20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/41808engRibeiro, Artur J.; Volkov, V.; Oliveira, Mariana B.; Padrão, Jorge; Mano, João F.; Gomes, Andreia C.; Cavaco-Paulo, Artur, BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds. International Journal of Biological Macromolecules, 89, 535-544, 20160141-813010.1016/j.ijbiomac.2016.05.01227156695http://www.elsevier.com/locate/issn/01418130info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-07T01:20:22Zoai:repositorium.sdum.uminho.pt:1822/41808Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:09.967953Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
title BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
spellingShingle BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
Ribeiro, Artur J.
Tunable scaffolds
Hydrophobic/Hydrophilic ratio
Induced cold gelation
Surface wettability
Mechanical properties
Cell proliferation
Science & Technology
title_short BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
title_full BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
title_fullStr BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
title_full_unstemmed BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
title_sort BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
author Ribeiro, Artur J.
author_facet Ribeiro, Artur J.
Volkov, Vadim
Oliveira, Mariana Braga
Padrão, Jorge
Mano, J. F.
Gomes, Andreia C
Cavaco-Paulo, Artur
author_role author
author2 Volkov, Vadim
Oliveira, Mariana Braga
Padrão, Jorge
Mano, J. F.
Gomes, Andreia C
Cavaco-Paulo, Artur
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ribeiro, Artur J.
Volkov, Vadim
Oliveira, Mariana Braga
Padrão, Jorge
Mano, J. F.
Gomes, Andreia C
Cavaco-Paulo, Artur
dc.subject.por.fl_str_mv Tunable scaffolds
Hydrophobic/Hydrophilic ratio
Induced cold gelation
Surface wettability
Mechanical properties
Cell proliferation
Science & Technology
topic Tunable scaffolds
Hydrophobic/Hydrophilic ratio
Induced cold gelation
Surface wettability
Mechanical properties
Cell proliferation
Science & Technology
description "Available online 6 May 2016"
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/41808
url https://hdl.handle.net/1822/41808
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ribeiro, Artur J.; Volkov, V.; Oliveira, Mariana B.; Padrão, Jorge; Mano, João F.; Gomes, Andreia C.; Cavaco-Paulo, Artur, BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds. International Journal of Biological Macromolecules, 89, 535-544, 2016
0141-8130
10.1016/j.ijbiomac.2016.05.012
27156695
http://www.elsevier.com/locate/issn/01418130
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132282641973248

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