Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/103357 https://doi.org/10.3390/md20040224 |
Resumo: | The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluorescence under UV light. Emission has two main maxima, at 400 nm and at 500 nm, with the latter responsible for the blue-greenish fluorescence. Combining proteomics and transcriptomics techniques, we identified ubiquitin, peroxiredoxin, and 14-3-3 protein as key elements in the mucus. Fluorescence was found to be mainly modulated by redox status and pH, being consistently upheld in extracts prepared in Tris-HCl buffer with reducing agent at pH 7 and excited at 330 nm. One of the proteins associated with the fluorescent signal was localized in secretory cells in the pharynx. The results indicate that the secretion of fluorescent proteinaceous complexes can be an important defense against UV for this dweller. Additionally, the internalization of fluorescent complexes by ovarian cancer cells and modulation of fluorescence of redox status bears important considerations for biotechnological application of mucus components as markers. |
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Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for BiotechnologyfluorescencemucosecretionsUV lightubiquitin conjugatesmarine Annelidaredox statusproteomemass spectrometrypoisonous secretionstranscriptomicsAnimalsBiotechnologyColoring AgentsHumansMucusPlant ExtractsProteinsAnnelidaPolychaetaThe vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluorescence under UV light. Emission has two main maxima, at 400 nm and at 500 nm, with the latter responsible for the blue-greenish fluorescence. Combining proteomics and transcriptomics techniques, we identified ubiquitin, peroxiredoxin, and 14-3-3 protein as key elements in the mucus. Fluorescence was found to be mainly modulated by redox status and pH, being consistently upheld in extracts prepared in Tris-HCl buffer with reducing agent at pH 7 and excited at 330 nm. One of the proteins associated with the fluorescent signal was localized in secretory cells in the pharynx. The results indicate that the secretion of fluorescent proteinaceous complexes can be an important defense against UV for this dweller. Additionally, the internalization of fluorescent complexes by ovarian cancer cells and modulation of fluorescence of redox status bears important considerations for biotechnological application of mucus components as markers.2022-03-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/103357http://hdl.handle.net/10316/103357https://doi.org/10.3390/md20040224eng1660-3397Rodrigo, Ana P.Lopes, AnaPereira, RicardoAnjo, Sandra I.Manadas, BrunoGrosso, Ana R.Baptista, Pedro V.Fernandes, Alexandra R.Costa, Pedro M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-08T21:33:49Zoai:estudogeral.uc.pt:10316/103357Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:20:12.678464Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology |
title |
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology |
spellingShingle |
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology Rodrigo, Ana P. fluorescence mucosecretions UV light ubiquitin conjugates marine Annelida redox status proteome mass spectrometry poisonous secretions transcriptomics Animals Biotechnology Coloring Agents Humans Mucus Plant Extracts Proteins Annelida Polychaeta |
title_short |
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology |
title_full |
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology |
title_fullStr |
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology |
title_full_unstemmed |
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology |
title_sort |
Endogenous Fluorescent Proteins in the Mucus of an Intertidal Polychaeta: Clues for Biotechnology |
author |
Rodrigo, Ana P. |
author_facet |
Rodrigo, Ana P. Lopes, Ana Pereira, Ricardo Anjo, Sandra I. Manadas, Bruno Grosso, Ana R. Baptista, Pedro V. Fernandes, Alexandra R. Costa, Pedro M. |
author_role |
author |
author2 |
Lopes, Ana Pereira, Ricardo Anjo, Sandra I. Manadas, Bruno Grosso, Ana R. Baptista, Pedro V. Fernandes, Alexandra R. Costa, Pedro M. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigo, Ana P. Lopes, Ana Pereira, Ricardo Anjo, Sandra I. Manadas, Bruno Grosso, Ana R. Baptista, Pedro V. Fernandes, Alexandra R. Costa, Pedro M. |
dc.subject.por.fl_str_mv |
fluorescence mucosecretions UV light ubiquitin conjugates marine Annelida redox status proteome mass spectrometry poisonous secretions transcriptomics Animals Biotechnology Coloring Agents Humans Mucus Plant Extracts Proteins Annelida Polychaeta |
topic |
fluorescence mucosecretions UV light ubiquitin conjugates marine Annelida redox status proteome mass spectrometry poisonous secretions transcriptomics Animals Biotechnology Coloring Agents Humans Mucus Plant Extracts Proteins Annelida Polychaeta |
description |
The vast ocean holds many unexplored organisms with unique adaptive features that enable them to thrive in their environment. The secretion of fluorescent proteins is one of them, with reports on the presence of such compounds in marine annelids being scarce. The intertidal Eulalia sp. is an example. The worm secretes copious amounts of mucus, that when purified and concentrated extracts, yield strong fluorescence under UV light. Emission has two main maxima, at 400 nm and at 500 nm, with the latter responsible for the blue-greenish fluorescence. Combining proteomics and transcriptomics techniques, we identified ubiquitin, peroxiredoxin, and 14-3-3 protein as key elements in the mucus. Fluorescence was found to be mainly modulated by redox status and pH, being consistently upheld in extracts prepared in Tris-HCl buffer with reducing agent at pH 7 and excited at 330 nm. One of the proteins associated with the fluorescent signal was localized in secretory cells in the pharynx. The results indicate that the secretion of fluorescent proteinaceous complexes can be an important defense against UV for this dweller. Additionally, the internalization of fluorescent complexes by ovarian cancer cells and modulation of fluorescence of redox status bears important considerations for biotechnological application of mucus components as markers. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/103357 http://hdl.handle.net/10316/103357 https://doi.org/10.3390/md20040224 |
url |
http://hdl.handle.net/10316/103357 https://doi.org/10.3390/md20040224 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1660-3397 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134095180038144 |