Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.)
Main Author: | |
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Publication Date: | 2020 |
Other Authors: | , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/68748 |
Summary: | A wide range of naturally derived and synthetic biodegradable and biocompatible polymers are today regarded as promising materials for improving skin regeneration. Alongside this, these materials have been explored in conjunction with different types of antimicrobial and bioactive agents, especially natural-derived compounds, to enhance their biological properties. Herein, a double-layered nanocomposite dressing membrane was fabricated with two distinct layers. A bottom layer from Chitosan-Sodium tripolyphosphate (CS-TPP) and Poly(vinyl alcohol) (PVA) containing <i>Centella asiatica</i> (L.) (CA) was electrospun directly over a Polycaprolactone (PCL) layer to improve the biologic performance of the electrospun nanofibers. In turn, the PCL layer was designed to provide mechanical support to the damaged tissue. The results revealed that the produced double-layered nanocomposite membrane closely resembles the mechanical, porosity, and wettability features required for skin tissue engineering. On the other hand, the in vitro drug release profile of the PCL/PVA_CS-TPP containing CA exhibited a controlled release for 10 days. Moreover, the PVA_CS-TPP_CA’s bottom layer displayed the highest antibacterial activity against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (99.96 ± 6.04%) and <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) (99.94 ± 0.67%), which is responsible for avoiding bacterial penetration while endowing bioactive properties. Finally, the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that this nanocomposite membrane was not cytotoxic for normal human dermal fibroblasts (NHDF) cells. Therefore, these findings suggest the potential use of the double-layered PCL/PVA_CS-TPP_CA as an efficient bionanocomposite dressing material. |
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Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.)polycaprolactonechitosansodium tripolyphosphatepoly(vinyl alcohol)Centella asiaticadouble-layered nanocomposite membranesScience & TechnologyA wide range of naturally derived and synthetic biodegradable and biocompatible polymers are today regarded as promising materials for improving skin regeneration. Alongside this, these materials have been explored in conjunction with different types of antimicrobial and bioactive agents, especially natural-derived compounds, to enhance their biological properties. Herein, a double-layered nanocomposite dressing membrane was fabricated with two distinct layers. A bottom layer from Chitosan-Sodium tripolyphosphate (CS-TPP) and Poly(vinyl alcohol) (PVA) containing <i>Centella asiatica</i> (L.) (CA) was electrospun directly over a Polycaprolactone (PCL) layer to improve the biologic performance of the electrospun nanofibers. In turn, the PCL layer was designed to provide mechanical support to the damaged tissue. The results revealed that the produced double-layered nanocomposite membrane closely resembles the mechanical, porosity, and wettability features required for skin tissue engineering. On the other hand, the in vitro drug release profile of the PCL/PVA_CS-TPP containing CA exhibited a controlled release for 10 days. Moreover, the PVA_CS-TPP_CA’s bottom layer displayed the highest antibacterial activity against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (99.96 ± 6.04%) and <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) (99.94 ± 0.67%), which is responsible for avoiding bacterial penetration while endowing bioactive properties. Finally, the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that this nanocomposite membrane was not cytotoxic for normal human dermal fibroblasts (NHDF) cells. Therefore, these findings suggest the potential use of the double-layered PCL/PVA_CS-TPP_CA as an efficient bionanocomposite dressing material.The authors are grateful for the support given by the FibEnTech Research Unit (Project UIDB/00195/2020). Cláudia Mouro also acknowledges a PhD fellowship from the Foundation for Science and Technology (FCT) (PD/BD/113550/2015).Multidisciplinary Digital Publishing InstituteUniversidade do MinhoMouro, CláudiaFangueiro, RaúlGouveia, Isabel C.20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/68748engMouro, C.; Fangueiro, R.; Gouveia, I.C. Preparation and Characterization of Electrospun Double-layered Nanocomposites Membranes as a Carrier for Centella asiatica (L.). Polymers 2020, 12, 2653.2073-436010.3390/polym12112653https://www.mdpi.com/2073-4360/12/11/2653info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:28Zoai:repositorium.sdum.uminho.pt:1822/68748Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:50:22.827507Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.) |
title |
Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.) |
spellingShingle |
Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.) Mouro, Cláudia polycaprolactone chitosan sodium tripolyphosphate poly(vinyl alcohol) Centella asiatica double-layered nanocomposite membranes Science & Technology |
title_short |
Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.) |
title_full |
Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.) |
title_fullStr |
Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.) |
title_full_unstemmed |
Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.) |
title_sort |
Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for centella asiatica (L.) |
author |
Mouro, Cláudia |
author_facet |
Mouro, Cláudia Fangueiro, Raúl Gouveia, Isabel C. |
author_role |
author |
author2 |
Fangueiro, Raúl Gouveia, Isabel C. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Mouro, Cláudia Fangueiro, Raúl Gouveia, Isabel C. |
dc.subject.por.fl_str_mv |
polycaprolactone chitosan sodium tripolyphosphate poly(vinyl alcohol) Centella asiatica double-layered nanocomposite membranes Science & Technology |
topic |
polycaprolactone chitosan sodium tripolyphosphate poly(vinyl alcohol) Centella asiatica double-layered nanocomposite membranes Science & Technology |
description |
A wide range of naturally derived and synthetic biodegradable and biocompatible polymers are today regarded as promising materials for improving skin regeneration. Alongside this, these materials have been explored in conjunction with different types of antimicrobial and bioactive agents, especially natural-derived compounds, to enhance their biological properties. Herein, a double-layered nanocomposite dressing membrane was fabricated with two distinct layers. A bottom layer from Chitosan-Sodium tripolyphosphate (CS-TPP) and Poly(vinyl alcohol) (PVA) containing <i>Centella asiatica</i> (L.) (CA) was electrospun directly over a Polycaprolactone (PCL) layer to improve the biologic performance of the electrospun nanofibers. In turn, the PCL layer was designed to provide mechanical support to the damaged tissue. The results revealed that the produced double-layered nanocomposite membrane closely resembles the mechanical, porosity, and wettability features required for skin tissue engineering. On the other hand, the in vitro drug release profile of the PCL/PVA_CS-TPP containing CA exhibited a controlled release for 10 days. Moreover, the PVA_CS-TPP_CA’s bottom layer displayed the highest antibacterial activity against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (99.96 ± 6.04%) and <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) (99.94 ± 0.67%), which is responsible for avoiding bacterial penetration while endowing bioactive properties. Finally, the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that this nanocomposite membrane was not cytotoxic for normal human dermal fibroblasts (NHDF) cells. Therefore, these findings suggest the potential use of the double-layered PCL/PVA_CS-TPP_CA as an efficient bionanocomposite dressing material. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/68748 |
url |
http://hdl.handle.net/1822/68748 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mouro, C.; Fangueiro, R.; Gouveia, I.C. Preparation and Characterization of Electrospun Double-layered Nanocomposites Membranes as a Carrier for Centella asiatica (L.). Polymers 2020, 12, 2653. 2073-4360 10.3390/polym12112653 https://www.mdpi.com/2073-4360/12/11/2653 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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