Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/25923 |
Resumo: | In the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the “ultrasonic emulsification method”. It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA–RNA complex was successfully introduced into mammalian (human) U2OS osteosarcoma cells and Trypanosoma brucei parasites. Using PVA coating of the RNA–BSA spheres we have achieved a significant increase in the number of microspheres penetrating mammalian cells. The mechanism of RNA encapsulation and the structure of the RNA–BSA complex are reported. |
id |
RCAP_b5df9906fba3c628ced571003afbab34 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/25923 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification methodScience & TechnologyIn the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the “ultrasonic emulsification method”. It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA–RNA complex was successfully introduced into mammalian (human) U2OS osteosarcoma cells and Trypanosoma brucei parasites. Using PVA coating of the RNA–BSA spheres we have achieved a significant increase in the number of microspheres penetrating mammalian cells. The mechanism of RNA encapsulation and the structure of the RNA–BSA complex are reported.Ulyana Shimanovich thanks Ministry of Science and Technology, Israel for the "Woman in Science" scholarship (3-8219).Royal Society of ChemistryUniversidade do MinhoShimanovich, UlyanaEliaz, DrorZigdon, SallyVolkov, VadimAizer, AdvaPaulo, Artur CavacoMichaeli, S.Shav-Tal, YaronGedanken, Aharon20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25923eng2050-751810.1039/C2TB00012Ainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:26:45Zoai:repositorium.sdum.uminho.pt:1822/25923Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:21:14.366141Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method |
title |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method |
spellingShingle |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method Shimanovich, Ulyana Science & Technology |
title_short |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method |
title_full |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method |
title_fullStr |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method |
title_full_unstemmed |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method |
title_sort |
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method |
author |
Shimanovich, Ulyana |
author_facet |
Shimanovich, Ulyana Eliaz, Dror Zigdon, Sally Volkov, Vadim Aizer, Adva Paulo, Artur Cavaco Michaeli, S. Shav-Tal, Yaron Gedanken, Aharon |
author_role |
author |
author2 |
Eliaz, Dror Zigdon, Sally Volkov, Vadim Aizer, Adva Paulo, Artur Cavaco Michaeli, S. Shav-Tal, Yaron Gedanken, Aharon |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Shimanovich, Ulyana Eliaz, Dror Zigdon, Sally Volkov, Vadim Aizer, Adva Paulo, Artur Cavaco Michaeli, S. Shav-Tal, Yaron Gedanken, Aharon |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
In the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the “ultrasonic emulsification method”. It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA–RNA complex was successfully introduced into mammalian (human) U2OS osteosarcoma cells and Trypanosoma brucei parasites. Using PVA coating of the RNA–BSA spheres we have achieved a significant increase in the number of microspheres penetrating mammalian cells. The mechanism of RNA encapsulation and the structure of the RNA–BSA complex are reported. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/25923 |
url |
http://hdl.handle.net/1822/25923 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2050-7518 10.1039/C2TB00012A |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132678628311040 |