Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats

Detalhes bibliográficos
Autor(a) principal: Oliveira, Paulo J.
Data de Publicação: 2003
Outros Autores: Seiça, Raquel, Coxito, Pedro M., Rolo, Anabela P., Palmeira, Carlos M., Santos, Maria S., Moreno, António J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/5393
https://doi.org/10.1016/S0014-5793(03)01233-X
Resumo: Cardiac dysfunction is associated with diabetes. It was previously shown that heart mitochondria from diabetic rats have a reduced calcium accumulation capacity. The objective of this work was to determine whether the reduction in calcium accumulation by cardiac mitochondria from diabetic rats is related to an enhanced susceptibility to induction of the mitochondrial permeability transition. Streptozotocin-induced diabetic rats were used as a model to study the alterations caused by diabetes in the permeability transition, 21 days after streptozotocin administration. Heart mitochondria were isolated to evaluate respiratory parameters and susceptibility to the calcium-dependent permeability transition. Our results show that streptozotocin diabetes facilitates the mitochondrial permeability transition in cardiac mitochondria, resulting in decreased mitochondrial calcium accumulation. We also observed that heart mitochondria from diabetic rats had depressed oxygen consumption during the phosphorylative state. The reduced mitochondrial calcium uptake observed in heart mitochondria from diabetic rats is related to an enhanced susceptibility to the permeability transition rather than to damage to the calcium uptake machinery.
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spelling Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic ratsMitochondrionDiabetesStreptozotocinPermeability transitionCardiac dysfunction is associated with diabetes. It was previously shown that heart mitochondria from diabetic rats have a reduced calcium accumulation capacity. The objective of this work was to determine whether the reduction in calcium accumulation by cardiac mitochondria from diabetic rats is related to an enhanced susceptibility to induction of the mitochondrial permeability transition. Streptozotocin-induced diabetic rats were used as a model to study the alterations caused by diabetes in the permeability transition, 21 days after streptozotocin administration. Heart mitochondria were isolated to evaluate respiratory parameters and susceptibility to the calcium-dependent permeability transition. Our results show that streptozotocin diabetes facilitates the mitochondrial permeability transition in cardiac mitochondria, resulting in decreased mitochondrial calcium accumulation. We also observed that heart mitochondria from diabetic rats had depressed oxygen consumption during the phosphorylative state. The reduced mitochondrial calcium uptake observed in heart mitochondria from diabetic rats is related to an enhanced susceptibility to the permeability transition rather than to damage to the calcium uptake machinery.http://www.sciencedirect.com/science/article/B6T36-49WPBSY-B/1/f91ef5d310996f93c4e23fb3df5856272003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5393http://hdl.handle.net/10316/5393https://doi.org/10.1016/S0014-5793(03)01233-XengFEBS Letters. 554:3 (2003) 511-514Oliveira, Paulo J.Seiça, RaquelCoxito, Pedro M.Rolo, Anabela P.Palmeira, Carlos M.Santos, Maria S.Moreno, António J. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-07T10:32:20Zoai:estudogeral.uc.pt:10316/5393Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:28.384602Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats
title Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats
spellingShingle Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats
Oliveira, Paulo J.
Mitochondrion
Diabetes
Streptozotocin
Permeability transition
title_short Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats
title_full Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats
title_fullStr Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats
title_full_unstemmed Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats
title_sort Enhanced permeability transition explains the reduced calcium uptake in cardiac mitochondria from streptozotocin-induced diabetic rats
author Oliveira, Paulo J.
author_facet Oliveira, Paulo J.
Seiça, Raquel
Coxito, Pedro M.
Rolo, Anabela P.
Palmeira, Carlos M.
Santos, Maria S.
Moreno, António J. M.
author_role author
author2 Seiça, Raquel
Coxito, Pedro M.
Rolo, Anabela P.
Palmeira, Carlos M.
Santos, Maria S.
Moreno, António J. M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Paulo J.
Seiça, Raquel
Coxito, Pedro M.
Rolo, Anabela P.
Palmeira, Carlos M.
Santos, Maria S.
Moreno, António J. M.
dc.subject.por.fl_str_mv Mitochondrion
Diabetes
Streptozotocin
Permeability transition
topic Mitochondrion
Diabetes
Streptozotocin
Permeability transition
description Cardiac dysfunction is associated with diabetes. It was previously shown that heart mitochondria from diabetic rats have a reduced calcium accumulation capacity. The objective of this work was to determine whether the reduction in calcium accumulation by cardiac mitochondria from diabetic rats is related to an enhanced susceptibility to induction of the mitochondrial permeability transition. Streptozotocin-induced diabetic rats were used as a model to study the alterations caused by diabetes in the permeability transition, 21 days after streptozotocin administration. Heart mitochondria were isolated to evaluate respiratory parameters and susceptibility to the calcium-dependent permeability transition. Our results show that streptozotocin diabetes facilitates the mitochondrial permeability transition in cardiac mitochondria, resulting in decreased mitochondrial calcium accumulation. We also observed that heart mitochondria from diabetic rats had depressed oxygen consumption during the phosphorylative state. The reduced mitochondrial calcium uptake observed in heart mitochondria from diabetic rats is related to an enhanced susceptibility to the permeability transition rather than to damage to the calcium uptake machinery.
publishDate 2003
dc.date.none.fl_str_mv 2003
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/5393
http://hdl.handle.net/10316/5393
https://doi.org/10.1016/S0014-5793(03)01233-X
url http://hdl.handle.net/10316/5393
https://doi.org/10.1016/S0014-5793(03)01233-X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv FEBS Letters. 554:3 (2003) 511-514
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