Proteomic approach to the caracterization of unknown origin male infertility
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/135778 |
Resumo: | Infertility affects around 50 million couples worldwide, where male infertility contributes for half of all cases. When no clear causesfor infertilitycanbe found, it is designated of unknown origin(UOMI), including idiopathic(ID)and unexplained male infertility (UMI). Diagnosis of male infertility isfirstlybased on routine semen analysis, an evaluationthat has been shown to havepoor prognostic value. This is especially evident in men with UMI that, despite presenting a normal seminal analysis, arestill infertile,stressing the need to deepen the analysis of sperm functionality in these patients to clarify what might be behind their infertility state.In this project,wefocusedon a detailed characterization of sperm function,that goes far beyondthe conventional analysis, to clarifythis issue. Besidesanalysing functional and bioenergetic parameters such as spermcapacitation, acrosome reaction, chromatin status and mitochondrial functionality, a sperm proteomic analysis was alsoperformedusing sequential window acquisition of all theoretical mass spectra (SWATH-MS). In addition, lifestyle and medical history of patients were assessed by survey. Symptomsof anxiety and depression were also evaluated through proper surveys.In this study, ID patients besides having significantly decreased sperm concentration, motility and morphology, also presented significantly decreased sperm viability, chromatin integrity and percentage of capacitated cells,comparing to healthy men, with the proteomic results further supporting the differences among these groups.Furthermore, ID patients had significantly increased incidence of urogenital infections and varicocele. Regarding UMI patients, we observedthat their sperm functionality is very similar to that of the healthy individuals, but significantly different from ID patients, whichwas also mirroredat the proteomic level.UMI patients were also observed to have increased incidence of diagnosed depression, when comparing to healthy individuals and ID patients. Finally, the proteins annexin A5, α-crystallin B chain, apolipoprotein H, destrin, NADH-cytochrome b5 reductase 3, platelet-activating factor acetylhydrolase IB subunit α1and transthyretinwere found, for the first time, to significantly differentiate the 3patient groups,hence being good candidates for further studies on UOMI.Overall, this study entailinga unique complete and integrated analysis of 3 groups of individuals’ sperm functionality and proteome, accuratelycategorized, providednew insights and add knowledge on these patients’ infertility unknown aetiology, opening road to future studies in the field. |
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Proteomic approach to the caracterization of unknown origin male infertilityUnknown origin male infertilityspermatozoaproteomicsmale infertilityCiências MédicasInfertility affects around 50 million couples worldwide, where male infertility contributes for half of all cases. When no clear causesfor infertilitycanbe found, it is designated of unknown origin(UOMI), including idiopathic(ID)and unexplained male infertility (UMI). Diagnosis of male infertility isfirstlybased on routine semen analysis, an evaluationthat has been shown to havepoor prognostic value. This is especially evident in men with UMI that, despite presenting a normal seminal analysis, arestill infertile,stressing the need to deepen the analysis of sperm functionality in these patients to clarify what might be behind their infertility state.In this project,wefocusedon a detailed characterization of sperm function,that goes far beyondthe conventional analysis, to clarifythis issue. Besidesanalysing functional and bioenergetic parameters such as spermcapacitation, acrosome reaction, chromatin status and mitochondrial functionality, a sperm proteomic analysis was alsoperformedusing sequential window acquisition of all theoretical mass spectra (SWATH-MS). In addition, lifestyle and medical history of patients were assessed by survey. Symptomsof anxiety and depression were also evaluated through proper surveys.In this study, ID patients besides having significantly decreased sperm concentration, motility and morphology, also presented significantly decreased sperm viability, chromatin integrity and percentage of capacitated cells,comparing to healthy men, with the proteomic results further supporting the differences among these groups.Furthermore, ID patients had significantly increased incidence of urogenital infections and varicocele. Regarding UMI patients, we observedthat their sperm functionality is very similar to that of the healthy individuals, but significantly different from ID patients, whichwas also mirroredat the proteomic level.UMI patients were also observed to have increased incidence of diagnosed depression, when comparing to healthy individuals and ID patients. Finally, the proteins annexin A5, α-crystallin B chain, apolipoprotein H, destrin, NADH-cytochrome b5 reductase 3, platelet-activating factor acetylhydrolase IB subunit α1and transthyretinwere found, for the first time, to significantly differentiate the 3patient groups,hence being good candidates for further studies on UOMI.Overall, this study entailinga unique complete and integrated analysis of 3 groups of individuals’ sperm functionality and proteome, accuratelycategorized, providednew insights and add knowledge on these patients’ infertility unknown aetiology, opening road to future studies in the field.Amaral, SandraBelo, JoséRUNPacheco, Rita Carolina2022-04-04T14:30:54Z2022-03-312022-03-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/135778TID:202983161enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:14:04Zoai:run.unl.pt:10362/135778Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:30.068685Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Proteomic approach to the caracterization of unknown origin male infertility |
title |
Proteomic approach to the caracterization of unknown origin male infertility |
spellingShingle |
Proteomic approach to the caracterization of unknown origin male infertility Pacheco, Rita Carolina Unknown origin male infertility spermatozoa proteomics male infertility Ciências Médicas |
title_short |
Proteomic approach to the caracterization of unknown origin male infertility |
title_full |
Proteomic approach to the caracterization of unknown origin male infertility |
title_fullStr |
Proteomic approach to the caracterization of unknown origin male infertility |
title_full_unstemmed |
Proteomic approach to the caracterization of unknown origin male infertility |
title_sort |
Proteomic approach to the caracterization of unknown origin male infertility |
author |
Pacheco, Rita Carolina |
author_facet |
Pacheco, Rita Carolina |
author_role |
author |
dc.contributor.none.fl_str_mv |
Amaral, Sandra Belo, José RUN |
dc.contributor.author.fl_str_mv |
Pacheco, Rita Carolina |
dc.subject.por.fl_str_mv |
Unknown origin male infertility spermatozoa proteomics male infertility Ciências Médicas |
topic |
Unknown origin male infertility spermatozoa proteomics male infertility Ciências Médicas |
description |
Infertility affects around 50 million couples worldwide, where male infertility contributes for half of all cases. When no clear causesfor infertilitycanbe found, it is designated of unknown origin(UOMI), including idiopathic(ID)and unexplained male infertility (UMI). Diagnosis of male infertility isfirstlybased on routine semen analysis, an evaluationthat has been shown to havepoor prognostic value. This is especially evident in men with UMI that, despite presenting a normal seminal analysis, arestill infertile,stressing the need to deepen the analysis of sperm functionality in these patients to clarify what might be behind their infertility state.In this project,wefocusedon a detailed characterization of sperm function,that goes far beyondthe conventional analysis, to clarifythis issue. Besidesanalysing functional and bioenergetic parameters such as spermcapacitation, acrosome reaction, chromatin status and mitochondrial functionality, a sperm proteomic analysis was alsoperformedusing sequential window acquisition of all theoretical mass spectra (SWATH-MS). In addition, lifestyle and medical history of patients were assessed by survey. Symptomsof anxiety and depression were also evaluated through proper surveys.In this study, ID patients besides having significantly decreased sperm concentration, motility and morphology, also presented significantly decreased sperm viability, chromatin integrity and percentage of capacitated cells,comparing to healthy men, with the proteomic results further supporting the differences among these groups.Furthermore, ID patients had significantly increased incidence of urogenital infections and varicocele. Regarding UMI patients, we observedthat their sperm functionality is very similar to that of the healthy individuals, but significantly different from ID patients, whichwas also mirroredat the proteomic level.UMI patients were also observed to have increased incidence of diagnosed depression, when comparing to healthy individuals and ID patients. Finally, the proteins annexin A5, α-crystallin B chain, apolipoprotein H, destrin, NADH-cytochrome b5 reductase 3, platelet-activating factor acetylhydrolase IB subunit α1and transthyretinwere found, for the first time, to significantly differentiate the 3patient groups,hence being good candidates for further studies on UOMI.Overall, this study entailinga unique complete and integrated analysis of 3 groups of individuals’ sperm functionality and proteome, accuratelycategorized, providednew insights and add knowledge on these patients’ infertility unknown aetiology, opening road to future studies in the field. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-04T14:30:54Z 2022-03-31 2022-03-31T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/135778 TID:202983161 |
url |
http://hdl.handle.net/10362/135778 |
identifier_str_mv |
TID:202983161 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138086206046208 |