In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures

Detalhes bibliográficos
Autor(a) principal: Bessa, Maria João
Data de Publicação: 2021
Outros Autores: Brandão, Fátima, Fokkens, Paul H.B., Leseman, Daan L.A.C., Boere, A. John F., Cassee, Flemming R., Salmatonidis, Apostolos, Viana, Mar, Vulpoi, Adriana, Simon, Simion, Monfort, Eliseo, Teixeira, João Paulo, Fraga, Sónia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7912
Resumo: This article belongs to the Special Issue Engineered Nanomaterials Exposure and Risk Assessment: Occupational Health and Safety
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spelling In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged CulturesDNA DamageAir-Liquid InterfaceEngineered NanoparticlesNanoparticlesGenotoxicityIn Vitro CytotoxicitySubmerged CulturesGenotoxicidade AmbientalThis article belongs to the Special Issue Engineered Nanomaterials Exposure and Risk Assessment: Occupational Health and SafetyDiverse industries have already incorporated within their production processes engineered nanoparticles (ENP), increasing the potential risk of worker inhalation exposure. In vitro models have been widely used to investigate ENP toxicity. Air-liquid interface (ALI) cell cultures have been emerging as a valuable alternative to submerged cultures as they are more representative of the inhalation exposure to airborne nano-sized particles. We compared the in vitro toxicity of four ENP used as raw materials in the advanced ceramics sector in human alveolar epithelial-like cells cultured under submerged or ALI conditions. Submerged cultures were exposed to ENP liquid suspensions or to aerosolised ENP at ALI. Toxicity was assessed by determining LDH release, WST-1 metabolisation and DNA damage. Overall, cells were more sensitive to ENP cytotoxic effects when cultured and exposed under ALI. No significant cytotoxicity was observed after 24 h exposure to ENP liquid suspensions, although aerosolised ENP clearly affected cell viability and LDH release. In general, all ENP increased primary DNA damage regardless of the exposure mode, where an increase in DNA strand-breaks was only detected under submerged conditions. Our data show that at relevant occupational concentrations, the selected ENP exert mild toxicity to alveolar epithelial cells and exposure at ALI might be the most suitable choice when assessing ENP toxicity in respiratory models under realistic exposure conditions.This research was funded by CERASAFE (www.cerasafe.eu; accessed on 26 October 2021), with the support of ERA-NET SIINN (project id:16) and the Portuguese Foundation for Science and Technology (FCT; SIINN/0004/2014). This work was also supported by the NanoBioBarriers project (PTDC/MED-TOX/31162/2017), co-financed by the Operational Program for Competitiveness and Internationalization (POCI) through European Regional Development Funds (FEDER/FNR) and FCT; Spanish Ministry of Science and Innovation (projects PCIN-2015-173-C02-01 and CEX2018-000794- S-Severo Ochoa), and by the Romanian National Authority for Scientific Research and Innovation (CCCDI-UEFISCDI, project number 29/2016 within PNCDI III). M.J. Bessa (SFRH/BD/120646/2016) and F. Brandão (SFRH/BD/101060/2014) are recipients of FCT PhD scholarships under the framework of Human Capital Operating Program (POCH) and European Union funding. The Doctoral Program in Biomedical Sciences, of the ICBAS—University of Porto, offered additional funds. S. Fraga thanks FCT for funding through program DL 57/2016–Norma transitória (Ref. DL-57/INSA-06/2018). Thanks are also due to FCT/MCTES for the financial support to EPIUnit (UIDB/04750/2020).MDPIRepositório Científico do Instituto Nacional de SaúdeBessa, Maria JoãoBrandão, FátimaFokkens, Paul H.B.Leseman, Daan L.A.C.Boere, A. John F.Cassee, Flemming R.Salmatonidis, ApostolosViana, MarVulpoi, AdrianaSimon, SimionMonfort, EliseoTeixeira, João PauloFraga, Sónia2022-02-02T15:48:46Z2021-11-272021-11-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7912engNanomaterials (Basel). 2021 Nov 27;11(12):3225. doi: 10.3390/nano11123225.2079-499110.3390/nano11123225info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:15Zoai:repositorio.insa.pt:10400.18/7912Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:30.106573Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures
title In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures
spellingShingle In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures
Bessa, Maria João
DNA Damage
Air-Liquid Interface
Engineered Nanoparticles
Nanoparticles
Genotoxicity
In Vitro Cytotoxicity
Submerged Cultures
Genotoxicidade Ambiental
title_short In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures
title_full In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures
title_fullStr In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures
title_full_unstemmed In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures
title_sort In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Alveolar Epithelial Cells: Air-Liquid Interface versus Submerged Cultures
author Bessa, Maria João
author_facet Bessa, Maria João
Brandão, Fátima
Fokkens, Paul H.B.
Leseman, Daan L.A.C.
Boere, A. John F.
Cassee, Flemming R.
Salmatonidis, Apostolos
Viana, Mar
Vulpoi, Adriana
Simon, Simion
Monfort, Eliseo
Teixeira, João Paulo
Fraga, Sónia
author_role author
author2 Brandão, Fátima
Fokkens, Paul H.B.
Leseman, Daan L.A.C.
Boere, A. John F.
Cassee, Flemming R.
Salmatonidis, Apostolos
Viana, Mar
Vulpoi, Adriana
Simon, Simion
Monfort, Eliseo
Teixeira, João Paulo
Fraga, Sónia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Bessa, Maria João
Brandão, Fátima
Fokkens, Paul H.B.
Leseman, Daan L.A.C.
Boere, A. John F.
Cassee, Flemming R.
Salmatonidis, Apostolos
Viana, Mar
Vulpoi, Adriana
Simon, Simion
Monfort, Eliseo
Teixeira, João Paulo
Fraga, Sónia
dc.subject.por.fl_str_mv DNA Damage
Air-Liquid Interface
Engineered Nanoparticles
Nanoparticles
Genotoxicity
In Vitro Cytotoxicity
Submerged Cultures
Genotoxicidade Ambiental
topic DNA Damage
Air-Liquid Interface
Engineered Nanoparticles
Nanoparticles
Genotoxicity
In Vitro Cytotoxicity
Submerged Cultures
Genotoxicidade Ambiental
description This article belongs to the Special Issue Engineered Nanomaterials Exposure and Risk Assessment: Occupational Health and Safety
publishDate 2021
dc.date.none.fl_str_mv 2021-11-27
2021-11-27T00:00:00Z
2022-02-02T15:48:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7912
url http://hdl.handle.net/10400.18/7912
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanomaterials (Basel). 2021 Nov 27;11(12):3225. doi: 10.3390/nano11123225.
2079-4991
10.3390/nano11123225
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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