DNA damage induced by hydroquinone can be prevented by fungal detoxification

Detalhes bibliográficos
Autor(a) principal: Pereira, Pedro
Data de Publicação: 2014
Outros Autores: Enguita, Francisco J., Ferreira, João, Leitão, Ana Lúcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51319
Resumo: © 2014 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
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spelling DNA damage induced by hydroquinone can be prevented by fungal detoxificationCytotoxicityDetoxificationGenotoxicityHydroquinonePenicillium chrysogenum var. halophenolicum© 2014 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).Hydroquinone is a benzene metabolite with a wide range of industrial applications, which has potential for widespread human exposure; however, the toxicity of hydroquinone on human cells remains unclear. The aims of this study are to investigate the cytotoxicity and genotoxicity of hydroquinone in human primary fibroblasts and human colon cancer cells (HCT116). Low doses of hydroquinone (227-454 μM) reduce the viability of fibroblasts and HCT116 cells, determined by resazurin conversion, and induce genotoxic damage (DNA strand breaks), as assessed by alkaline comet assays. Bioremediation may provide an excellent alternative to promote the degradation of hydroquinone, however few microorganisms are known that efficiently degrade it. Here we also investigate the capacity of a halotolerant fungus, Penicillium chrysogenum var. halophenolicum, to remove hydroquinone toxicity under hypersaline condition. The fungus is able to tolerate high concentrations of hydroquinone and can reverse these noxious effects via degradation of hydroquinone to completion, even when the initial concentration of this compound is as high as 7265 μM. Our findings reveal that P. chrysogenum var. halophenolicum efficiently degrade hydroquinone under hypersaline conditions, placing this fungus among the best candidates for the detoxification of habitats contaminated with this aromatic compound.This work was partially supported by a Gulbenkian Foundation research grant (#96526/2009) awarded to JF, and PD received support from Fundação para a Ciência e Tecnologia/FCT-Portugal (SFRH/BD/45502/2008).ElsevierRepositório da Universidade de LisboaPereira, PedroEnguita, Francisco J.Ferreira, JoãoLeitão, Ana Lúcia2022-02-15T15:10:16Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51319engToxicol Rep. 2014 Nov 4;1:1096-110510.1016/j.toxrep.2014.10.0242214-7500info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:55:55Zoai:repositorio.ul.pt:10451/51319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:37.110546Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv DNA damage induced by hydroquinone can be prevented by fungal detoxification
title DNA damage induced by hydroquinone can be prevented by fungal detoxification
spellingShingle DNA damage induced by hydroquinone can be prevented by fungal detoxification
Pereira, Pedro
Cytotoxicity
Detoxification
Genotoxicity
Hydroquinone
Penicillium chrysogenum var. halophenolicum
title_short DNA damage induced by hydroquinone can be prevented by fungal detoxification
title_full DNA damage induced by hydroquinone can be prevented by fungal detoxification
title_fullStr DNA damage induced by hydroquinone can be prevented by fungal detoxification
title_full_unstemmed DNA damage induced by hydroquinone can be prevented by fungal detoxification
title_sort DNA damage induced by hydroquinone can be prevented by fungal detoxification
author Pereira, Pedro
author_facet Pereira, Pedro
Enguita, Francisco J.
Ferreira, João
Leitão, Ana Lúcia
author_role author
author2 Enguita, Francisco J.
Ferreira, João
Leitão, Ana Lúcia
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pereira, Pedro
Enguita, Francisco J.
Ferreira, João
Leitão, Ana Lúcia
dc.subject.por.fl_str_mv Cytotoxicity
Detoxification
Genotoxicity
Hydroquinone
Penicillium chrysogenum var. halophenolicum
topic Cytotoxicity
Detoxification
Genotoxicity
Hydroquinone
Penicillium chrysogenum var. halophenolicum
description © 2014 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2022-02-15T15:10:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51319
url http://hdl.handle.net/10451/51319
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicol Rep. 2014 Nov 4;1:1096-1105
10.1016/j.toxrep.2014.10.024
2214-7500
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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