Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/44379 |
Resumo: | The integrity and function of the periodontium can be compromised by traumatic injuries or periodontitis. Currently available clinical therapies are able to stop the progression of periodontitis and allow the healing of periodontal tissue. However, an optimal strategy capable of restoring the anatomy and functionality of the lost periodontal tissue is still to be achieved. Herein is proposed the development of an injectable hydrogel system able to release a growth factors and cells to the periodontal defect. This injectable system is based on a photocrosslinkable hydrogel, prepared from methacrylated hyaluronic acid (me-HA) and incorporating platelet lysate (PL). The delivery of growth factors and cells in situ is expected to enhance regeneration of the periodontium. Various formulations of me-HA containing increasing PL concentrations were studied for achieving the formation of stable photocrosslinkable hydrogels. The produced hydrogels were subsequently characterized to assess mechanical properties, degradation, protein/growth factor release profile, antimicrobial activity and response toward human Periodontal Ligament fibroblasts (hPDLFs). The results demonstrated that it was possible to obtain stable photocrosslinkable hydrogels incorporating different amounts of PL that can be released in a sustained manner. Furthermore, the incorporation of PL improved (p < 0.02) the viscoelastic properties of the hydrogels and enhanced their resilience to the degradation by hyaluronidase (HAase). Additionally, the PL was shown to provide antimicrobial properties. Finally, hPDLFs, either seeded or encapsulated into the developed hydrogels, showed enhanced proliferation over time (p < 0.05), proportionally to the increasing amounts of PL present in the hydrogel formulations. |
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Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technologyPhoto-crosslinkable hydrogelsPlatelet lysateHyaluronic acid,Periodontal ligamentEndogenous regenerative technologyScience & TechnologyThe integrity and function of the periodontium can be compromised by traumatic injuries or periodontitis. Currently available clinical therapies are able to stop the progression of periodontitis and allow the healing of periodontal tissue. However, an optimal strategy capable of restoring the anatomy and functionality of the lost periodontal tissue is still to be achieved. Herein is proposed the development of an injectable hydrogel system able to release a growth factors and cells to the periodontal defect. This injectable system is based on a photocrosslinkable hydrogel, prepared from methacrylated hyaluronic acid (me-HA) and incorporating platelet lysate (PL). The delivery of growth factors and cells in situ is expected to enhance regeneration of the periodontium. Various formulations of me-HA containing increasing PL concentrations were studied for achieving the formation of stable photocrosslinkable hydrogels. The produced hydrogels were subsequently characterized to assess mechanical properties, degradation, protein/growth factor release profile, antimicrobial activity and response toward human Periodontal Ligament fibroblasts (hPDLFs). The results demonstrated that it was possible to obtain stable photocrosslinkable hydrogels incorporating different amounts of PL that can be released in a sustained manner. Furthermore, the incorporation of PL improved (p < 0.02) the viscoelastic properties of the hydrogels and enhanced their resilience to the degradation by hyaluronidase (HAase). Additionally, the PL was shown to provide antimicrobial properties. Finally, hPDLFs, either seeded or encapsulated into the developed hydrogels, showed enhanced proliferation over time (p < 0.05), proportionally to the increasing amounts of PL present in the hydrogel formulations.The research leading to these results has received funding from Fundacao para a Ciencia e a Tecnologia (FCT) under project BIBS (PTDC/CVT/102972/2008) and project ACROSS (PTDC/BBB-BIO/0827/2012), from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement number REGPOT-CT2012-316331-PO-LARIS and from the project "Novel smart and biomimetic materials for innovative regenerative medicine approaches" RL1-ABMR-NORTE-01-0124-FEDER-000016 cofinanced by North Portugal Regional Operational Programme (ON.2-O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF).ACS PublicationsUniversidade do MinhoBabo, Pedro Miguel SousaPires, R. L.Santos, LíviaFranco, Albina RibeiroRodrigues, F.Leonor, I. B.Reis, R. L.Gomes, Manuela E.20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44379engBabo P. S., Pires R. L., Santos L., Franco A. R., Rodrigues F., Leonor I. B., Reis R. L., Gomes M. E. Platelet Lysate-Loaded Photo-crosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology, ACS Biomaterials Science & Engineering, doi:10.1021/acsbiomaterials.6b00508, 2017.2373-987810.1021/acsbiomaterials.6b00508http://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.6b00508info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:26:46Zoai:repositorium.sdum.uminho.pt:1822/44379Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:21:14.890123Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology |
title |
Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology |
spellingShingle |
Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology Babo, Pedro Miguel Sousa Photo-crosslinkable hydrogels Platelet lysate Hyaluronic acid, Periodontal ligament Endogenous regenerative technology Science & Technology |
title_short |
Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology |
title_full |
Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology |
title_fullStr |
Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology |
title_full_unstemmed |
Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology |
title_sort |
Platelet lysate-loaded photo-crosslinkable hyaluronic acid hydrogels for periodontal endogenous regenerative technology |
author |
Babo, Pedro Miguel Sousa |
author_facet |
Babo, Pedro Miguel Sousa Pires, R. L. Santos, Lívia Franco, Albina Ribeiro Rodrigues, F. Leonor, I. B. Reis, R. L. Gomes, Manuela E. |
author_role |
author |
author2 |
Pires, R. L. Santos, Lívia Franco, Albina Ribeiro Rodrigues, F. Leonor, I. B. Reis, R. L. Gomes, Manuela E. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Babo, Pedro Miguel Sousa Pires, R. L. Santos, Lívia Franco, Albina Ribeiro Rodrigues, F. Leonor, I. B. Reis, R. L. Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Photo-crosslinkable hydrogels Platelet lysate Hyaluronic acid, Periodontal ligament Endogenous regenerative technology Science & Technology |
topic |
Photo-crosslinkable hydrogels Platelet lysate Hyaluronic acid, Periodontal ligament Endogenous regenerative technology Science & Technology |
description |
The integrity and function of the periodontium can be compromised by traumatic injuries or periodontitis. Currently available clinical therapies are able to stop the progression of periodontitis and allow the healing of periodontal tissue. However, an optimal strategy capable of restoring the anatomy and functionality of the lost periodontal tissue is still to be achieved. Herein is proposed the development of an injectable hydrogel system able to release a growth factors and cells to the periodontal defect. This injectable system is based on a photocrosslinkable hydrogel, prepared from methacrylated hyaluronic acid (me-HA) and incorporating platelet lysate (PL). The delivery of growth factors and cells in situ is expected to enhance regeneration of the periodontium. Various formulations of me-HA containing increasing PL concentrations were studied for achieving the formation of stable photocrosslinkable hydrogels. The produced hydrogels were subsequently characterized to assess mechanical properties, degradation, protein/growth factor release profile, antimicrobial activity and response toward human Periodontal Ligament fibroblasts (hPDLFs). The results demonstrated that it was possible to obtain stable photocrosslinkable hydrogels incorporating different amounts of PL that can be released in a sustained manner. Furthermore, the incorporation of PL improved (p < 0.02) the viscoelastic properties of the hydrogels and enhanced their resilience to the degradation by hyaluronidase (HAase). Additionally, the PL was shown to provide antimicrobial properties. Finally, hPDLFs, either seeded or encapsulated into the developed hydrogels, showed enhanced proliferation over time (p < 0.05), proportionally to the increasing amounts of PL present in the hydrogel formulations. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/44379 |
url |
http://hdl.handle.net/1822/44379 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Babo P. S., Pires R. L., Santos L., Franco A. R., Rodrigues F., Leonor I. B., Reis R. L., Gomes M. E. Platelet Lysate-Loaded Photo-crosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology, ACS Biomaterials Science & Engineering, doi:10.1021/acsbiomaterials.6b00508, 2017. 2373-9878 10.1021/acsbiomaterials.6b00508 http://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.6b00508 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ACS Publications |
publisher.none.fl_str_mv |
ACS Publications |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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