Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/72548 |
Resumo: | Nisin Z, an amphipathic peptide, with a significant antibacterial activity against Gram-positive bacteria and low toxicity in humans, has been studied for food preservation applications. Thus far, very little research has been done to explore its potential in biomedicine. Here, we report the modification of sodium alginate (SA) and gelatin (GN) blended microfibers, produced via the wet-spinning technique, with Nisin Z, with the purpose of eradicating <i>Staphylococcus aureus</i>-induced infections. Wet-spun SAGN microfibers were successfully produced at a 70/30% <i>v</i>/<i>v</i> of SA (2 wt%)/GN (1 wt%) polymer ratio by extrusion within a calcium chloride (CaCl<sub>2</sub>) coagulation bath. Modifications to the biodegradable fibers’ chemical stability and structure were then introduced via crosslinking with CaCl<sub>2</sub> and glutaraldehyde (SAGNCL). Regardless of the chemical modification employed, all microfibers were labelled as homogeneous both in size (≈246.79 µm) and shape (cylindrical and defect-free). SA-free microfibers, with an increased surface area for peptide immobilization, originated from the action of phosphate buffer saline solution on SAGN fibers, were also produced (GNCL). Their durability in physiological conditions (simulated body fluid) was, however, compromised very early in the experiment (day 1 and 3, with and without Nisin Z, respectively). Only the crosslinked SAGNCL fibers remained intact for the 28 day-testing period. Their thermal resilience in comparison with the unmodified and SA-free fibers was also demonstrated. Nisin Z was functionalized onto the unmodified and chemically altered fibers at an average concentration of 178 µg/mL. Nisin Z did not impact on the fiber’s morphology nor on their chemical/thermal stability. However, the peptide improved the SA fibers (control) structural integrity, guaranteeing its stability for longer, in physiological conditions. Its main effect was detected on the time-kill kinetics of the bacteria <i>S. aureus</i>. SAGNCL and GNCL loaded with Nisin Z were capable of progressively eliminating the bacteria, reaching an inhibition superior to 99% after 24 h of culture. The peptide-modified SA and SAGN were not as effective, losing their antimicrobial action after 6 h of incubation. Bacteria elimination was consistent with the release kinetics of Nisin Z from the fibers. In general, data revealed the increased potential and durable effect of Nisin Z (significantly superior to its free, unloaded form) against <i>S. aureus</i>-induced infections, while loaded onto prospective biomedical wet-spun scaffolds. |
id |
RCAP_c00598ac12f4f9c99af3efe7013ae195 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/72548 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
|
spelling |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infectionsAntimicrobial peptideBiodegradable microfibersCalcium chlorideGlutaraldehyde crosslinkingMicrofiber functionalizationBactericidal actionScience & TechnologyNisin Z, an amphipathic peptide, with a significant antibacterial activity against Gram-positive bacteria and low toxicity in humans, has been studied for food preservation applications. Thus far, very little research has been done to explore its potential in biomedicine. Here, we report the modification of sodium alginate (SA) and gelatin (GN) blended microfibers, produced via the wet-spinning technique, with Nisin Z, with the purpose of eradicating <i>Staphylococcus aureus</i>-induced infections. Wet-spun SAGN microfibers were successfully produced at a 70/30% <i>v</i>/<i>v</i> of SA (2 wt%)/GN (1 wt%) polymer ratio by extrusion within a calcium chloride (CaCl<sub>2</sub>) coagulation bath. Modifications to the biodegradable fibers’ chemical stability and structure were then introduced via crosslinking with CaCl<sub>2</sub> and glutaraldehyde (SAGNCL). Regardless of the chemical modification employed, all microfibers were labelled as homogeneous both in size (≈246.79 µm) and shape (cylindrical and defect-free). SA-free microfibers, with an increased surface area for peptide immobilization, originated from the action of phosphate buffer saline solution on SAGN fibers, were also produced (GNCL). Their durability in physiological conditions (simulated body fluid) was, however, compromised very early in the experiment (day 1 and 3, with and without Nisin Z, respectively). Only the crosslinked SAGNCL fibers remained intact for the 28 day-testing period. Their thermal resilience in comparison with the unmodified and SA-free fibers was also demonstrated. Nisin Z was functionalized onto the unmodified and chemically altered fibers at an average concentration of 178 µg/mL. Nisin Z did not impact on the fiber’s morphology nor on their chemical/thermal stability. However, the peptide improved the SA fibers (control) structural integrity, guaranteeing its stability for longer, in physiological conditions. Its main effect was detected on the time-kill kinetics of the bacteria <i>S. aureus</i>. SAGNCL and GNCL loaded with Nisin Z were capable of progressively eliminating the bacteria, reaching an inhibition superior to 99% after 24 h of culture. The peptide-modified SA and SAGN were not as effective, losing their antimicrobial action after 6 h of incubation. Bacteria elimination was consistent with the release kinetics of Nisin Z from the fibers. In general, data revealed the increased potential and durable effect of Nisin Z (significantly superior to its free, unloaded form) against <i>S. aureus</i>-induced infections, while loaded onto prospective biomedical wet-spun scaffolds.This research received funding from the Portuguese Foundation for Science and Technology (FCT) under the scope of the projects PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074) and UID/CTM/00264/2021.Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoHomem, Natália CândidoTavares, Tânia Daniela EugénioMiranda, Catarina S.Antunes, Joana Isabel CostaAmorim, M. T. Pessoa deFelgueiras, Helena Prado2021-02-162021-02-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/72548engHomem, N.C.; Tavares, T.D.; Miranda, C.S.; Antunes, J.C.; Amorim, M.T.P.; Felgueiras, H.P. Functionalization of Crosslinked Sodium Alginate/Gelatin Wet-Spun Porous Fibers with Nisin Z for the Inhibition of Staphylococcus aureus-Induced Infections. Int. J. Mol. Sci. 2021, 22, 1930. https://doi.org/10.3390/ijms220419301661-65961422-006710.3390/ijms2204193033669209https://www.mdpi.com/1422-0067/22/4/1930info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:14:57ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections |
title |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections |
spellingShingle |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections Homem, Natália Cândido Antimicrobial peptide Biodegradable microfibers Calcium chloride Glutaraldehyde crosslinking Microfiber functionalization Bactericidal action Science & Technology |
title_short |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections |
title_full |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections |
title_fullStr |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections |
title_full_unstemmed |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections |
title_sort |
Functionalization of crosslinked sodium alginate/gelatin wet-spun porous fibers with Nisin Z for the inhibition of Staphylococcus aureus-induced infections |
author |
Homem, Natália Cândido |
author_facet |
Homem, Natália Cândido Tavares, Tânia Daniela Eugénio Miranda, Catarina S. Antunes, Joana Isabel Costa Amorim, M. T. Pessoa de Felgueiras, Helena Prado |
author_role |
author |
author2 |
Tavares, Tânia Daniela Eugénio Miranda, Catarina S. Antunes, Joana Isabel Costa Amorim, M. T. Pessoa de Felgueiras, Helena Prado |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Homem, Natália Cândido Tavares, Tânia Daniela Eugénio Miranda, Catarina S. Antunes, Joana Isabel Costa Amorim, M. T. Pessoa de Felgueiras, Helena Prado |
dc.subject.por.fl_str_mv |
Antimicrobial peptide Biodegradable microfibers Calcium chloride Glutaraldehyde crosslinking Microfiber functionalization Bactericidal action Science & Technology |
topic |
Antimicrobial peptide Biodegradable microfibers Calcium chloride Glutaraldehyde crosslinking Microfiber functionalization Bactericidal action Science & Technology |
description |
Nisin Z, an amphipathic peptide, with a significant antibacterial activity against Gram-positive bacteria and low toxicity in humans, has been studied for food preservation applications. Thus far, very little research has been done to explore its potential in biomedicine. Here, we report the modification of sodium alginate (SA) and gelatin (GN) blended microfibers, produced via the wet-spinning technique, with Nisin Z, with the purpose of eradicating <i>Staphylococcus aureus</i>-induced infections. Wet-spun SAGN microfibers were successfully produced at a 70/30% <i>v</i>/<i>v</i> of SA (2 wt%)/GN (1 wt%) polymer ratio by extrusion within a calcium chloride (CaCl<sub>2</sub>) coagulation bath. Modifications to the biodegradable fibers’ chemical stability and structure were then introduced via crosslinking with CaCl<sub>2</sub> and glutaraldehyde (SAGNCL). Regardless of the chemical modification employed, all microfibers were labelled as homogeneous both in size (≈246.79 µm) and shape (cylindrical and defect-free). SA-free microfibers, with an increased surface area for peptide immobilization, originated from the action of phosphate buffer saline solution on SAGN fibers, were also produced (GNCL). Their durability in physiological conditions (simulated body fluid) was, however, compromised very early in the experiment (day 1 and 3, with and without Nisin Z, respectively). Only the crosslinked SAGNCL fibers remained intact for the 28 day-testing period. Their thermal resilience in comparison with the unmodified and SA-free fibers was also demonstrated. Nisin Z was functionalized onto the unmodified and chemically altered fibers at an average concentration of 178 µg/mL. Nisin Z did not impact on the fiber’s morphology nor on their chemical/thermal stability. However, the peptide improved the SA fibers (control) structural integrity, guaranteeing its stability for longer, in physiological conditions. Its main effect was detected on the time-kill kinetics of the bacteria <i>S. aureus</i>. SAGNCL and GNCL loaded with Nisin Z were capable of progressively eliminating the bacteria, reaching an inhibition superior to 99% after 24 h of culture. The peptide-modified SA and SAGN were not as effective, losing their antimicrobial action after 6 h of incubation. Bacteria elimination was consistent with the release kinetics of Nisin Z from the fibers. In general, data revealed the increased potential and durable effect of Nisin Z (significantly superior to its free, unloaded form) against <i>S. aureus</i>-induced infections, while loaded onto prospective biomedical wet-spun scaffolds. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-16 2021-02-16T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/72548 |
url |
http://hdl.handle.net/1822/72548 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Homem, N.C.; Tavares, T.D.; Miranda, C.S.; Antunes, J.C.; Amorim, M.T.P.; Felgueiras, H.P. Functionalization of Crosslinked Sodium Alginate/Gelatin Wet-Spun Porous Fibers with Nisin Z for the Inhibition of Staphylococcus aureus-Induced Infections. Int. J. Mol. Sci. 2021, 22, 1930. https://doi.org/10.3390/ijms22041930 1661-6596 1422-0067 10.3390/ijms22041930 33669209 https://www.mdpi.com/1422-0067/22/4/1930 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1777303714447491072 |