L-asparaginase-based biosensors
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/32884 |
Resumo: | L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 ºC, its use as a biosensor in the detection and monitoring of L-asparagine levels is of high relevance. ASNase-based biosensors are a promising and innovative technology, mostly based on colorimetric detection since the mechanism of action of ASNase is the catalysis of the L-asparagine hydrolysis, which releases L-aspartic acid and ammonium ions, promoting a medium pH value change followed by color variation. ASNase biosensing systems prove their potential for L-asparagine monitoring in ALL patients, along with L-asparagine concentration analysis in foods, due to their simplicity and fast response. |
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L-asparaginase-based biosensorsL-asparaginaseBiosensorL-asparagineMonitoringAmmoniaPharmaceuticalFoodIndustryL-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 ºC, its use as a biosensor in the detection and monitoring of L-asparagine levels is of high relevance. ASNase-based biosensors are a promising and innovative technology, mostly based on colorimetric detection since the mechanism of action of ASNase is the catalysis of the L-asparagine hydrolysis, which releases L-aspartic acid and ammonium ions, promoting a medium pH value change followed by color variation. ASNase biosensing systems prove their potential for L-asparagine monitoring in ALL patients, along with L-asparagine concentration analysis in foods, due to their simplicity and fast response.MDPI2022-01-12T15:23:09Z2021-08-20T00:00:00Z2021-08-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/32884eng2673-839210.3390/encyclopedia1030065Nunes, João C. F.Cristóvão, Raquel O.Santos-Ebinuma, Valéria C.Faria, Joaquim L.Silva, Cláudia G.Neves, Márcia C.Freire, Mara G.Tavares, Ana P. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-17T04:11:57ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
L-asparaginase-based biosensors |
title |
L-asparaginase-based biosensors |
spellingShingle |
L-asparaginase-based biosensors Nunes, João C. F. L-asparaginase Biosensor L-asparagine Monitoring Ammonia Pharmaceutical Food Industry |
title_short |
L-asparaginase-based biosensors |
title_full |
L-asparaginase-based biosensors |
title_fullStr |
L-asparaginase-based biosensors |
title_full_unstemmed |
L-asparaginase-based biosensors |
title_sort |
L-asparaginase-based biosensors |
author |
Nunes, João C. F. |
author_facet |
Nunes, João C. F. Cristóvão, Raquel O. Santos-Ebinuma, Valéria C. Faria, Joaquim L. Silva, Cláudia G. Neves, Márcia C. Freire, Mara G. Tavares, Ana P. M. |
author_role |
author |
author2 |
Cristóvão, Raquel O. Santos-Ebinuma, Valéria C. Faria, Joaquim L. Silva, Cláudia G. Neves, Márcia C. Freire, Mara G. Tavares, Ana P. M. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Nunes, João C. F. Cristóvão, Raquel O. Santos-Ebinuma, Valéria C. Faria, Joaquim L. Silva, Cláudia G. Neves, Márcia C. Freire, Mara G. Tavares, Ana P. M. |
dc.subject.por.fl_str_mv |
L-asparaginase Biosensor L-asparagine Monitoring Ammonia Pharmaceutical Food Industry |
topic |
L-asparaginase Biosensor L-asparagine Monitoring Ammonia Pharmaceutical Food Industry |
description |
L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 ºC, its use as a biosensor in the detection and monitoring of L-asparagine levels is of high relevance. ASNase-based biosensors are a promising and innovative technology, mostly based on colorimetric detection since the mechanism of action of ASNase is the catalysis of the L-asparagine hydrolysis, which releases L-aspartic acid and ammonium ions, promoting a medium pH value change followed by color variation. ASNase biosensing systems prove their potential for L-asparagine monitoring in ALL patients, along with L-asparagine concentration analysis in foods, due to their simplicity and fast response. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-20T00:00:00Z 2021-08-20 2022-01-12T15:23:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/32884 |
url |
http://hdl.handle.net/10773/32884 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2673-8392 10.3390/encyclopedia1030065 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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